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Lrrk2

Manufactured by Sino Biological
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LRRK2 is a laboratory protein produced by Sino Biological. It is a kinase enzyme that plays a role in various cellular processes. The core function of LRRK2 is to phosphorylate specific target proteins, though its precise biological role is an area of ongoing research.

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Lab products found in correlation

Transcend non radioactive translation detection system, by Promega (2 mentions) Tnt quick coupled transcription translation kit, by Promega (2 mentions) Recombinant flag gpnmb, by OriGene (2 mentions) Gst egfr, by Active Motif (2 mentions) Recombinant active caspase 1, by R&D Systems (2 mentions) Protein purification kit, by Takara Bio (2 mentions) Bl21 codonplus de3 ripl, by Agilent Technologies (2 mentions) Hif 1α, by Novus Biologicals (2 mentions)

2 protocols using lrrk2

1

Recombinant Protein Expression and Purification

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Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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2

Recombinant Protein Expression and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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