Acetone, n-hexane, ethyl acetate, concentrated sulfuric acid, sodium hydroxide, phosphoric acid, acetonitrile, ascorbic acid, methanol, formic acid, sodium acetate, acetic acid, hydrochloric acid, iron(III) chloride hexahydrate solution, ammonium acetate, were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). phenolic compound standards [rutin (Rut), quercetin (Que), quercitrin (Quer), isoquercitrin (Iso), chlorogenic acid (ChA), syringic acid (SyA), ferulic acid (FeA), caffeic acid (CaA), resveratrol (Res), epicatechin (Epi), astragalin (Ast), scopoletin (Sco), galangal (Gal), catechuic acid (CatA), vanillic acid (VaA), benzoic acid (BeA), gallic acid (GaA), and protocatechuic acid (PrA)] were purchased from the National Institutes for Food and Drug Control (Beijing, China). 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2ʹ-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Methanol
Manufactured by Guangzhou Chemical
Sourced in China
Methanol is a colorless, volatile, and flammable liquid commonly used as a laboratory solvent and reagent. It has the chemical formula CH3OH. Methanol is a simple alcohol with a wide range of applications in various industries, including as a fuel additive, a feedstock for the production of other chemicals, and a solvent for paints, resins, and other products.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Guangzhou Chemical (20 mentions)
Acetone, by Guangzhou Chemical (19 mentions)
Hydrochloric acid (hcl), by Guangzhou Chemical (10 mentions)
Ethyl acetate, by Guangzhou Chemical (10 mentions)
Phosphoric acid, by Guangzhou Chemical (10 mentions)
Acetonitrile, by Guangzhou Chemical (10 mentions)
Formic acid, by Guangzhou Chemical (10 mentions)
Acetic acid, by Guangzhou Chemical (9 mentions)
Concentrated sulfuric acid, by Guangzhou Chemical (9 mentions)
Sodium hydroxide (naoh), by Guangzhou Chemical (9 mentions)
Ammonium acetate, by Guangzhou Chemical (9 mentions)
Ascorbic acid, by Guangzhou Chemical (9 mentions)
Zinc nitrate hexahydrate, by Guangzhou Chemical (9 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (3 mentions)
N hexane, by Guangzhou Chemical (3 mentions)
Transwell chambers 8 μm, by Corning (3 mentions)
Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (3 mentions)
2 methylimidazole 2 mim, by Maclin Biochemical Technology (3 mentions)
Urea, by Maclin Biochemical Technology (3 mentions)
Co no3 2 6h2o, by Maclin Biochemical Technology (3 mentions)
12 protocols using methanol
1
Phytochemical Profiling and Antioxidant Evaluation
2
Synthesis of Metal-Doped Silica Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cd(NO3)2, Pb(NO3)2, Mg(NO3)2, Zn(NO3)2, MnSO4, Ni(NO3)2 and Cu(NO3)2 were supplied by Aladdin Reagent Company (Shanghai, China). Ferric and ferrous chlorides (FeCl3·6H2O and FeCl2·4H2O, respectively) were obtained from Tianjin Chemical Reagent Co. (China). Ethylenediamine tetra-acetic acid (EDTA, AR), ammonium ferric sulfate (NH4FeSO4·H2O, AR), ammonia (NH3·H2O, AR), sodium hydroxide (NaOH, AR), methanol and ethanol were purchased from Guangzhou Chemical Reagent Factory (GCRF). Tetraethyl orthosilicate (TEOS) was obtained from Shanghai Chemical Reagent Factory. The waste beer yeast was from Zhaoqing Pabst Blue Ribbon Co.
3
Colony Formation and Cell Migration Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SW1990 cells were plated in 6-well plates (500 cells/well), followed by incubation for 2-3 weeks. Cells were fixed with 4% formaldehyde and stained with crystal violet. A number of colonies with more than 10 cells were counted under the microscope with colony formation rate = (colony number/seeded cell number) × 100% [37 (link)].
Transwell chambers (8 μm, Corning, NY) were applied for cell migration evaluation [38 (link)]. SW1990 cells resuspended with 200 μl serum-free medium were plated in the upper chamber, with 10% FBS medium added in lower chamber. After 24 h, cells were fixed in methanol (Guangzhou Chemical Reagent Factory, Guangzhou, China) for 30 min. The crystal violet- (0.1%) stained cells were counted under an inverted microscope (Caikon Optical Instrument Co. Ltd., Shanghai, China).
Transwell chambers (8 μm, Corning, NY) were applied for cell migration evaluation [38 (link)]. SW1990 cells resuspended with 200 μl serum-free medium were plated in the upper chamber, with 10% FBS medium added in lower chamber. After 24 h, cells were fixed in methanol (Guangzhou Chemical Reagent Factory, Guangzhou, China) for 30 min. The crystal violet- (0.1%) stained cells were counted under an inverted microscope (Caikon Optical Instrument Co. Ltd., Shanghai, China).
4
Antimicrobial Nanomaterial Synthesis and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc nitrate hexahydrate (ZnNO3·6H2O, 99%), ethanol (CH3CH3O, 99.7%) and methanol (CH3OH, 99.5%) were purchased from Guang Zhou Chemical Reagent Factory. 2-Methylimidazole (2-MIM, 98%) was purchased from Macklin. Curcumin (CCM, 98%) and indocyanine green (ICG, 75%) were purchased from Aladdin. Bacterial culture media was purchased from Huan Kai Microbial. Gram-positive bacteria (S. aureus, ATCC 23235) and Gram-negative bacteria (E. coli, ATCC 43894) were obtained from China Center of Industrial Culture. LIVE/DEAD Bac Light Bacterial Viability Kit was purchased from Thermo Fisher Scientific.
5
Biobased Furans Synthesis from Carbohydrates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Co (NO3)2•6H2O, 2-methylimidazole, 5-hydroxymethylfurfural (97.0%), 5-methylfurfural, 2-butanol, urea, and melamine were procured from Shanghai Macklin Biochemical Co. Cu (NO3)2•3H2O, methanol, tetrahydrofuran (THF), and isopropanol (IPA) were procured from Guangzhou Chemical Reagent Factory. Glucose was procured from Tianjin Zhiyuan Chemical Reagent Co. Ltd.
6
Gelatin-Silk Hydrogel Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gelatin (type A, 300 g bloom) was purchased from Sigma Aldrich. Degummed silk was purchased from Simatech Incorporation, China. LiBr, N-(3-Dimethylaminopropyl)-N'ethylcarbodiimide hydrochloride (EDC), N-Hydroxysulfosuccinimide sodium salt (NHS), tyramine hydrochloride, morpholinoethanesulfnic acid (MES), and HRP (300 units/mg) were purchased from Aladdin Chemical Reagent Co., Ltd., China. Methanol and aqueous H2O2 solution (30% (w/w)) were purchased from Guangzhou Chemical Reagent Factory, China.
7
Catalyst Preparation for Aromatic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Guaiacol, phenol, anisole, and 1,3,5-trimethoxybenzene, and γ-Al2O3 were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Acetone and methanol were obtained from the Guangzhou Chemical Reagent Factory. These chemicals were directly used without any pretreatment. SiO2–Al2O3 was purchased from Shanxi Juhua New Material Technology Co., Ltd, which was named x-SA (x is the SiO2 content). All catalysts were pressed and sieved to 40–60 mesh before use and calcined at 550 °C for 3 hours.
8
Optimizing Cell Culture and Viability Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phosphate-buffered saline (PBS), Trypsin/EDTA solution, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) double antibody were purchased from Gibco. Accutase® solution and NaAsO 2 were purchased from Sigma-Aldrich. Endothelial cell medium (ECM) was purchased from ScienCell Research Laboratories. Primary antibodies (anti-vimentin and anti-E-cadherin) were purchased from Cell Signaling Technologies. Additional Primary antibodies (anti-CD34, anti-N-cadherin) were purchased from Abcam. A Cell Counting Kit-8 (CCK-8) was purchased from DOJINDO. Zn(NO 3 ) 2 •6H 2 O, 2-methylimidazole, polyethylene glycol (2000), indocyanine green (ICG) and fluorescein isothiocyanate (FITC) were purchased from Aladdin. Methanol was purchased from Guangzhou Chemical Reagent Factory. An Annexin V-FITC/PI apoptosis detection kit was purchased from BD Biosciences. A calcein-AM/PI double staining kit was purchased from Solarbio, Beijing.
9
Comprehensive Chemical Reagents Inventory
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium chloride, anhydrous ethanol, ethanol, methanol, anhydrous sodium sulfite, sodium thiosulfate, and glacial acetic acid were purchased from Guangzhou Chemical Reagent (China). Diethyl Phosphate buffer solution (PBS, pH 7.4), pyr-ocarbonate (DEPC) and polyoxyethy-lene sorbitan monolaurate (Tween-20) were purchased from Sangon Biotech (Shanghai, China). Other chemicals and reagents were all from Sigma (St. Louis, MO, USA).
10
Optimized Mycoplasma gallisepticum Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mycoplasma gallisepticum strain S6 was obtained from the Chinese Veterinary Microorganism Culture Collection Center (Beijing, China). Culture media consisted of M. gallisepticum artificial medium base (Qingdao Hope Biological Technology) containing nicotinamide adenine dinucleotide and cysteine and swine serum (Guangzhou Ruite Biological) as previously described (3 (link)). Tilmicosin phosphate was a kind gift of Eastern Along Pharmaceuticals (Guangdong, China). Acetonitrile, formic acid, methanol (high-performance liquid chromatography grade), and remaining analytical grade reagents were purchased from Guangzhou Chemical Reagent.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!