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Gelatin coated 96 well plates

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The Gelatin-coated 96-well plates are a type of laboratory equipment designed for cell culture and research applications. These plates feature a gelatin coating, which provides a suitable substrate for cell adhesion and growth. The core function of these plates is to facilitate the culturing and study of cells in a standardized and controlled environment.

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Lab products found in correlation

X gal, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Gelatin-coated black-walled 96-well plates Gelatin-coated plates 96-well plates Gelatin

2 protocols using gelatin coated 96 well plates

1

Assessing Retinoic Acid Production in Monocytes

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The Sil-15 cell line (24 (link)), kindly provided by Dr. Michael Wagner, SUNY Downstate Medical Center, Brooklyn, NY, was used to assess RA production by monocytes recovered from epithelial cell co-culture experiments. Sil-15 cells were grown on gelatin-coated 96-well plates (BD Labware, Bedford, MA) in DMEM supplemented with 20% FBS and 1% G418 (Mediatech, Inc., Manassas, VA). Cellular populations to be tested were added at 100,000/well to confluent monolayers of Sil-15 cells. After overnight incubation, supernatants were removed, and Sil-15 cells were lysed by three freeze-thaw cycles in PBS. β-Galactosidase activity in Sil-15 lysates was then determined using X-Gal (1 mg/mL; Thermo Scientific, Waltham, MA) in developer solution made of 5 mM K3[Fe(CN)6], 5 mM K4[Fe(CN)6] and 2 mM MgCl2 in PBS, and color development was measured at 630 nm. RA production was calculated as RA equivalents based on a standard curve generated with known amounts of RA (detection range 50 pM – 100 nM).
Bimczok D., Kao J.Y., Zhang M., Cochrun S., Mannon P., Peter S., Wilcox C.M., Mönkemüller K.E., Harris P.R., Grams J.M., Stahl R.D., Smith P.D, & Smythies L.E. (2014). Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells. Mucosal immunology, 8(3), 533-544.
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2

Assessing Retinoic Acid Production in Monocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Sil-15 cell line (24 (link)), kindly provided by Dr. Michael Wagner, SUNY Downstate Medical Center, Brooklyn, NY, was used to assess RA production by monocytes recovered from epithelial cell co-culture experiments. Sil-15 cells were grown on gelatin-coated 96-well plates (BD Labware, Bedford, MA) in DMEM supplemented with 20% FBS and 1% G418 (Mediatech, Inc., Manassas, VA). Cellular populations to be tested were added at 100,000/well to confluent monolayers of Sil-15 cells. After overnight incubation, supernatants were removed, and Sil-15 cells were lysed by three freeze-thaw cycles in PBS. β-Galactosidase activity in Sil-15 lysates was then determined using X-Gal (1 mg/mL; Thermo Scientific, Waltham, MA) in developer solution made of 5 mM K3[Fe(CN)6], 5 mM K4[Fe(CN)6] and 2 mM MgCl2 in PBS, and color development was measured at 630 nm. RA production was calculated as RA equivalents based on a standard curve generated with known amounts of RA (detection range 50 pM – 100 nM).
Bimczok D., Kao J.Y., Zhang M., Cochrun S., Mannon P., Peter S., Wilcox C.M., Mönkemüller K.E., Harris P.R., Grams J.M., Stahl R.D., Smith P.D, & Smythies L.E. (2014). Human Gastric Epithelial Cells Contribute to Gastric Immune Regulation by Providing Retinoic Acid to Dendritic Cells. Mucosal immunology, 8(3), 533-544.
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