Atto 647n pe

Manufactured by ATTO-TEC

Sourced in Germany

ATTO-647N-PE is a fluorescent dye with an excitation maximum at 647 nm and an emission maximum at 667 nm. It is used as a labeling agent for various biomolecules such as proteins, nucleic acids, and other biological samples for applications in fluorescence-based techniques.

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Lab products found in correlation

Sodium cholate, by Merck Group (1 mentions) Soy pc, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocoline popc, by Avanti Polar Lipids (1 mentions) Atto488 dppe, by ATTO-TEC (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions) Mpb pe, by Avanti Polar Lipids (1 mentions)

2 protocols using atto 647n pe

Fluorescently Labeled Lipid Protocols

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Lissamine-rhodamin B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (RhB-PE) was purchased from Invitrogen (Copenhagen, Denmark). ATTO-647N-PE and ATTO-488-DPPE (ATTO-488 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine) was purchased from ATTO-TEC GmbH (Siegen, Germany). 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline (POPC) and Soy PC (catalog #840054) were from Avanti Polar Lipids (Alabaster, AL). Sodium cholate and all other chemicals used were obtained from Sigma-Aldrich (Copenhagen, Denmark). The buffer used was 10 mM phosphate buffered saline (PBS), 137 mM NaCl, and 2.7 mM KCl, pH 7.4.

Dreier J., Sørensen J.A, & Brewer J.R. (2016). Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier. PLoS ONE, 11(1), e0146514.

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Lipid Reagents for Membrane Studies

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1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), DOGS–NTA–Ni²⁺, Rhod–PE, and MPB–PE were purchased from Avanti Polar Lipids. ATTO647N-PE was purchased from ATTO-TEC.

Milanini J., Magdeleine M., Fuggetta N., Ikhlef S., Brau F., Abelanet S., Alpy F., Tomasetto C, & Drin G. (2022). In situ artificial contact sites (ISACS) between synthetic and endogenous organelle membranes allow for quantification of protein-tethering activities. The Journal of Biological Chemistry, 298(5), 101780.

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