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2 protocols using purasorb pdlg 5004a

1

Encapsulation of GLP-1 and DPP4 Inhibitor

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GLP-1 acetate (7–37, MW 3355.7 Da) was purchased from United Peptides (USA). iDPP4 (NVP DPP 728 dihydrochloride, MW 375.77 Da) was purchased from Tocris Bioscience (UK) and CPP R9 was purchased from GenicBio (China). PLGA 50 : 50 was obtained from Corbion Purac, Purasorb® PDLG 5004A, The Netherlands. Polyvinyl alcohol (PVA), medium molecular weight CS, 2-(N-morpholino)-ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), N-hydroxysuccinimide (NHS), STZ, nicotinamide and isoflurane were purchased from Sigma-Aldrich (USA). HPMC-AS was obtained from Shin-Etsu (Japan). Pluronic® F127 was purchased from BASF (Germany). Lancet Unistik 2 Normal Fixed Depth Lancet Needle 2.4 mm 21 Gauge was purchased from Owen Mumford, Ltd (France) and FreeStyle Precision Blood Glucose Test Strips from Abbott Diabetes Care (Portugal).
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2

Antibiotic-Loaded Polymeric Nanoparticles

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Type A gelatin from porcine skin (G1890), methacrylic anhydride (94%), 2-Hydroxy-4-(2-hydroxyethoxy)- 2-methylpropiophenone (Irgacure 2959, 98%), O-Phthaldialdehyde (OPA), butylamine, Span 80, rifampicin (Rif), vancomycin (Van), and sodium borate were ordered from Sigma-Aldrich (St. Louis, MO, United States. Poly (D,L-lactide-co-glycolide) (PLGA; acid endcap) low (LMW; AP081) and medium (MMW; AP041) molecular weight was purchased from Akina, Inc. (West Lafayette, IN, United States) and high molecular weight (HMW) Purasorb PDLG 5004A was purchased from Corbion (Amsterdam, the Netherlands). Phosphate-buffered saline (PBS) tablets were purchased from Carl Roth Gmbh (Karlsruhe, Germany). Polyvinyl alcohol (PVA) 87%–89% hydrolyzed 13–23 kDa was purchased from Acros Organics (Geel, Belgium). Sorbitan Monooleate (Span 80) was purchased from Glentham Life Science (Corsham, United Kingdom). Dichloromethane (DCM) was purchased from Stanlab (Lublin, Poland).
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