Hp innowax fused silica capillary column

Seed fatty acid composition was analyzed according to [26] with the following modification: the gas chromatography of the methyl esters was performed with a HP-INNOWax fused silica capillary column (0.25 mm by 0.5 m and 7.5 µm) (Agilent Technologies) at 250°C using hydrogen as the carrier gas. A minimum of 10 seeds from each of the parental lines and F₁ hybrids as well as 160 F₂ seeds of each of the three crosses were half-seed analyzed according to [27] . Ninety-six seeds from each of the BC₁ populations were analyzed using the single seed method.

GC-MS analysis was performed using a HP 7890B gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled with a 5977A mass selective detector (MSD) (Agilent Technologies) and a multi-purpose sampler MPS 2 (Gerstel, Mülheim an der Ruhr, Germany). HP-innowax fused silica capillary column (30 m length × 0.25 mm internal diameter × 0.25 μm film thickness; Agilent Technologies) was used with helium, a carrier gas, at a constant flow rate of 1 mL/min. Injection volume was 1.0 μL with split mode (20:1). The oven temperature program started at 40 °C initially and was held for 3 min, then was raised to 200 °C at 5 °C/min and held at 200 °C for 3 min. Post run was held at 180 °C for 2 min. Inlet, detector transfer line and mass source temperatures were 250, 280, 230 °C, respectively. MS was operated in the electron impact (EI) ion source mode at 70 eV with a scan range of 25~350 a.m.u. Ethanol (>99.9%) was purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA) for standard preparation. 4-Methyl-1-pentanol, an internal standard (ISTD) compound, was also obtained from Sigma-Aldrich. dl-alanine and dl-proline were purchased from Sigma-Aldrich and Oasis MAX cartridge 6 cc with stationary phase was purchased from Waters (Waters, Milford, MA, USA).

Seed fatty acid composition was analyzed according to Thies (1971)⁴¹ with the following modification: the gas chromatography of the methyl esters was performed with a HP-INNOWax fused silica capillary column (0.25 mm by 0.5 m and 7.5 µm) (Agilent Technologies) at 250 °C using hydrogen as the carrier gas. A minimum of 10 seeds from each of the parental lines and F₁ hybrids, and 150 F₂ seeds of each of the crosses Y1798 × Y1801, Y1798 × Y514 and Y1801 × Y514 were half-seed analyzed according to Downey and Harvey (1963)^{42 (link)}.