Mouse il 1β

Manufactured by BD

Sourced in United States, Australia

Mouse IL-1β is a recombinant protein that represents the mature form of the mouse interleukin-1 beta (IL-1β) cytokine. IL-1β is a key mediator of inflammatory responses and plays a central role in the regulation of immune and inflammatory processes.

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Lab products found in correlation

Mouse il 6, by BD (3 mentions) Mouse tnf α, by BD (3 mentions) Lsrfortessa flow cytometer, by BD (2 mentions) Cytometric bead array, by BD (2 mentions) Facsdiva v9, by BD (2 mentions) Mouse rat soluble protein master buffer kit, by BD (2 mentions) Mouse il 1α, by BD (2 mentions) Mouse il 12 il 23p40, by BD (2 mentions) Mouse il 10, by BD (2 mentions) Mouse ifn γ, by BD (2 mentions) Mouse il 12, by BD (1 mentions) Mouse cxcl10, by R&D Systems (1 mentions) Human il 6, by BD (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Mouse ifn β, by R&D Systems (1 mentions) Il 1β, by R&D Systems (1 mentions) Elisa reader, by Molecular Devices (1 mentions) Ifn β, by R&D Systems (1 mentions) Interleukin 6 (il 6), by BD (1 mentions) Cxcl1 kc, by R&D Systems (1 mentions)

Close spelling variants of this product

IL-1β (109 citations) Mouse IL-1β ELISA Set (13 citations) Mouse IL-1β ELISA kit (10 citations) OptEIA Mouse IL-1β Set (6 citations) BD OptEIA mouse IL-1β ELISA set (5 citations) CBA) Mouse IL-1β Flex Set (5 citations) Mouse IL-1β Flex Set (5 citations) Recombinant mouse IL-1β (3 citations) Mouse IL-1β ELISA (2 citations)

6 protocols using mouse il 1β

Cytokine Secretion Profiling by ELISA

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Mouse sera, tissue homogenates, or cell supernatants were used to examine level of cytokine secretion by ELISA. Commercial kits as followings were used according to manufacturer's protocols: mouse IFN‐β (PBL Interferon Source), mouse IL‐6 (BD Biosciences), mouse IL‐12 (BD Biosciences), mouse TNF‐α (BD Biosciences), mouse CCL5 (R&D Systems), mouse CXCL10 (R&D Systems), mouse IL‐1β (BD Biosciences), human IFN‐β (PBL interferon source), and human IL‐6 (BD Biosciences).

Chathuranga K., Kim T., Lee H., Park J., Kim J., Chathuranga W.A., Ekanayaka P., Choi Y.J., Lee C., Kim C., Jung J.U, & Lee J. (2020). Negative regulation of NEMO signaling by the ubiquitin E3 ligase MARCH2. The EMBO Journal, 39(21), e105139.

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Quantifying Inflammatory Cytokines

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Concentrations of MIP-2, IFN-β, IL-1β, and TNF were measured using ELISA kits for MIP-2 (R&D Systems, Minneapolis, MN, USA), mouse IFN-β (R&D Systems), mouse IL-1β (BD Biosciences, Franklin Lakes, NJ, USA), and mouse TNF (BD Biosciences), according to the manufacturers' instructions. Cytokine levels were estimated by interpolation from a standard curve generated using an ELISA reader (Molecular Devices) at 450 nm.

Lee H.R., Shin S.H., Kim J.H., Sohn K.Y., Yoon S.Y, & Kim J.W. (2019). 1-Palmitoyl-2-Linoleoyl-3-Acetyl-rac-Glycerol (PLAG) Rapidly Resolves LPS-Induced Acute Lung Injury Through the Effective Control of Neutrophil Recruitment. Frontiers in Immunology, 10, 2177.

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Cytokine Quantification in Cell Supernatants

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Supernatants from cell culture, plasma, or tissue cultures were assayed for mouse IL-1β (BD Biosciences, no. 559603), mouse IL-18 (eBioscience, no. BMS618/3) and IL-6 (BD Biosciences, no. 555240), according to the manufacturer’s instructions.

Shen L., Yang Y., Ou T., Key C.C., Tong S.H., Sequeira R.C., Nelson J.M., Nie Y., Wang Z., Boudyguina E., Shewale S.V, & Zhu X. (2017). Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy. Journal of Lipid Research, 58(9), 1808-1821.

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Quantification of Inflammatory Cytokines

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Mouse IL-1β (BD Biosciences, North Ryde, NSW, Australia), IL-18 (R&D Systems, In Vitro Technologies Pty Ltd., VIC, Australia), Cxcl1/KC (R&D Systems), Cxcl2/MIP2 (R&D Systems), human IL-18 (R&D Systems) and CXCL8 (BD Biosciences) were quantified by ELISA, as per the manufacturers’ instructions. Production of bioactive IL-18 by AGS cells was also measured using the HEK-Blue™ IL-18 reporter cell line system (InvivoGen, San Diego, CA, USA).

Tran L.S., Ying L., D’Costa K., Wray-McCann G., Kerr G., Le L., Allison C.C., Ferrand J., Chaudhry H., Emery J., De Paoli A., Colon N., Creed S., Kaparakis-Liaskos M., Como J., Dowling J.K., Johanesen P.A., Kufer T.A., Pedersen J.S., Mansell A., Philpott D.J., Elgass K.D., Abud H.E., Nachbur U., Croker B.A., Masters S.L, & Ferrero R.L. (2023). NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice. Nature Communications, 14, 3804.

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Cytokine Profiling in Mice Serum

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Serum supernatant was isolated from the inferior vena cava of euthanized mice and cytokines measured using the Cytometric Bead Array (BD Biosciences) with the amount of capture beads, detection reagents, and sample volumes scaled down tenfold from manufacturer’s protocol. Data was collected on an LSRFortessa flow cytometer (BD Biosciences) with FACSDiva v9.0 (BD Biosciences) and analyzed with FlowJo v10 (BD Biosciences). Statistical outliers were removed in GraphPad Prism v9.3 using ROUT method (Q=1%). Cytokine used were mouse TNFα (BD 558299), mouse IL-6 (BD 558301), mouse IL-1α (BD 560157), mouse IL-1β (BD 560232), mouse IL-10 (BD 558300), mouse IL-12/IL-23p40 (BD 560151), and mouse IFN-γ (BD 558296) with Mouse/Rat Soluble Protein Master Buffer Kit (BD 558266).

Danan C.H., Naughton K.E., Hayer K.E., Vellappan S., McMillan E.A., Zhou Y., Matsuda R., Nettleford S.K., Katada K., Parham L.R., Ma X., Chowdhury A., Wilkins B.J., Shah P., Weitzman M.D, & Hamilton K.E. (2023). Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survival. bioRxiv.

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Cytokine Profiling from Mouse Serum

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Serum supernatant was isolated from the inferior vena cava of euthanized mice, and cytokines measured using the Cytometric Bead Array (BD Biosciences), with the amount of capture beads, detection reagents, and sample volumes scaled down 10-fold from manufacturer’s protocol. Data were collected on an LSRFortessa flow cytometer (BD Biosciences) with FACSDiva v9.0 (BD Biosciences) and analyzed with FlowJo v10 (BD Biosciences). Statistical outliers were removed in GraphPad Prism v9.3 using ROUT method (Q = 1%). Cytokines used were mouse TNF-α (BD Biosciences 558299), mouse IL-6 (BD Biosciences 558301), mouse IL-1α (BD Biosciences 560157), mouse IL-1β (BD Biosciences 560232), mouse IL-10 (BD Biosciences 558300), mouse IL-12/IL-23p40 (BD Biosciences 560151), and mouse IFN-γ (BD Biosciences 558296) with Mouse/Rat Soluble Protein Master Buffer Kit (BD Biosciences 558266).

Danan C.H., Naughton K.E., Hayer K.E., Vellappan S., McMillan E.A., Zhou Y., Matsuda R., Nettleford S.K., Katada K., Parham L.R., Ma X., Chowdhury A., Wilkins B.J., Shah P., Weitzman M.D, & Hamilton K.E. (2023). Intestinal transit-amplifying cells require METTL3 for growth factor signaling and cell survival. JCI Insight, 8(23), e171657.

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