Use the Lamina Propria Dissociation Kit (Miltenyi biotech, North Rhine Westphalia, Germany) to dissociate the lamina propria cells of the dams intestine and prepare them into a single cell suspension for flow cytometry. The surface markers of intestinal lamina propria lymphocytes were stained as follows. For extracellular markers, single cells were stained at 2 × 106 cells per well in a 96-well V bottomed plate. T and B cells were stained with anti-CD3 BV421, anti-CD23 FITC, anti-CD21 BB700, anti-B220-PE, anti-CD19 APC, anti-CD45 APC-CY7 (BD, NJ, United States). DC and macrophage cells were stained with anti-F4/80 BV421, anti-MHCII PERCP-CY5.5, anti-CD11c APC (BD, NJ, United States). Cells were acquired on an LSRII (Becton Dickinson) and analyzed by FlowJo (Tree Star, Ashland).
Anti mhcii percp cy5
Manufactured by BD
Sourced in United States
Anti-MHCII PERCP-CY5.5 is a fluorophore-conjugated antibody that binds to major histocompatibility complex class II (MHCII) proteins. It is designed for use in flow cytometry applications to detect and analyze MHCII-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Lamina propria dissociation kit, by Miltenyi Biotec (1 mentions)
Anti cd19 apc, by BD (1 mentions)
Anti cd45 apc cy7, by BD (1 mentions)
Anti cd3 bv421, by BD (1 mentions)
Anti cd23 fitc, by BD (1 mentions)
Anti b220 pe, by BD (1 mentions)
Anti f4 80 bv421, by BD (1 mentions)
Anti cd11c apc, by BD (1 mentions)
Anti cd11b apc, by BD (1 mentions)
Facscanto 2 analyzer, by BD (1 mentions)
Anti cd80 fitc, by BD (1 mentions)
Anti cd11c pe, by BioLegend (1 mentions)
Canto 2 flow cytometer, by BD (1 mentions)
Anti cd11c apc, by Cytek Biosciences (1 mentions)
Anti cd80 pe cy7, by Thermo Fisher Scientific (1 mentions)
Anti cd86 pe, by BD (1 mentions)
3 protocols using anti mhcii percp cy5
1
Dissociation and Immunophenotyping of Intestinal Lamina Propria
2
MHCII Cells Analysis in Stromal Visceral Fat
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The stromal visceral fat fraction (SVF) and spleen were used for the analysis of major histocompatibility complex class II (MHCII) cells. For this purpose, the obtained fraction was stained with anti-MHCII-PercpCy5.5 (562363 BD), anti-CD11C-PE (117308 BioLegend), anti-CD80-FITC (553768 BD), and anti-CD11b-APC (553312 BD); washed; and resuspended in PBS-5% FBS. The samples were analyzed using a FACSCanto II analyzer (BD Bioscience). Data were analyzed using FlowJo software for flow cytometry analysis.
3
Flow Cytometric Characterization of BMDCs
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The BMDCs were stained with LIVE/DEAD Fixable Dead Cell Staining dye (Life Technologies, Carlsbad, CA, USA) to discriminate between the live and dead cells. The BMDCs were stained with anti-CD40 fluorescein isothiocyanate (FITC), anti-CD86 PE, anti-MHC II PerCP-Cy5.5 (BD Biosciences, San Jose, CA, USA), anti-CD80 PE-Cy7 (eBioscience, Santa Clara, CA, USA), anti-CD11c APC (Tonbo Biosciences, San Diego, CA, USA), and anti-CD11b APC-H7 (BioLegend, San Diego, CA, USA). The samples were run on a BD Canto II flow cytometer (BD Biosciences), and the data were analyzed using FlowJo software (Treestar, Ashland, OR, USA).
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