Anti paxillin and anti c jun

The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti ERK1/2, anti-JNK1/2, anti-phospho-JNK1/2 and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR (Thermo Fisher Scientific, Rockford, IL, USA); anti-EGFR, anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase-linked F(ab’)2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG₁ (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA), and Alexa Fluor 488 conjugated anti-mouse (from donkey) (Thermo Fisher Scientific, Rockford, IL, USA).

The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti ERK1/2, anti-JNK1/2, anti-phospho-JNK1/2 and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR (Thermo Fisher Scientific, Rockford, IL, USA); anti-EGFR, anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase-linked F(ab’)2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG₁ (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA), and Alexa Fluor 488 conjugated anti-mouse (from donkey) (Thermo Fisher Scientific, Rockford, IL, USA).

The following commercial primary antibodies were used: anti-phospho-ERK1/2, anti-ERK, anti-JNK1/2, anti-phospho-JNK1/2, and anti-phospho-c-Jun (all from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-EGFR, anti-EGFR, anti-FAK (all from Thermo Fisher Scientific, Rockford, IL, USA); anti-phospho-FAK (Abcam, Cambridge, MA, USA); anti-paxillin and anti-c-Jun (Santa Cruz Biotechnology, Heidelberg, Germany); anti-phospho-paxillin Ser 178 (Antibodies-online GmbH, Aachen, Germany); and anti-β-actin (Sigma-Aldrich, St Louis, MO, USA). Secondary antibodies were anti-rabbit IgG Horseradish peroxidase linked F(ab')2 I fragment (from donkey) (GE Healthcare, GE, Little Chalfont, United Kingdom), anti-mouse IgG₁ (BD Pharmingen, Beckton Dickinson, Franklin Lakes, NJ, USA); Alexa Fluor 488 conjugated anti-rabbit (from donkey), Alexa Fluor 488 conjugated anti-mouse (from donkey) and Alexa Fluor 594 conjugated anti-mouse (from donkey) (all from Thermo Fisher Scientific, Rockford, IL, USA).