β actin antibody ab8227

Manufactured by Abcam

Sourced in United States

The β-actin antibody (ab8227) is a primary antibody that recognizes the beta-actin protein, a widely expressed cytoskeletal protein. It can be used in various applications to detect and quantify beta-actin expression in biological samples.

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Lab products found in correlation

Pdzk1 antibody pab15564, by Abnova (2 mentions) Nhe3 antibody nhe31 a, by Alpha Diagnostic (2 mentions) Cell culture reagents, by GE Healthcare (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Las 3000 imaging system, by Fujifilm (1 mentions) Ab2105, by Abcam (1 mentions) Ab31828, by Abcam (1 mentions) Ab6672, by Abcam (1 mentions) Ab17942, by Abcam (1 mentions) Enhanced chemiluminescence system, by Cytiva (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Goat anti rabbit igg h l hrp, by Abcam (1 mentions) Ab86299, by Abcam (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)

Close spelling variants of this product

Anti-β-actin antibody (ab8227) β-actin (ab8227) β-actin antibody β-actin Ab8227

5 protocols using β actin antibody ab8227

Protein Extraction and Western Blot Analysis

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Total protein was extracted using RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) supplemented with protease and phosphatase inhibitors (ThermoFisher). Disruption and homogenization of tumor samples was achieved by the use of a TissueLyser II (Qiagen). Protein concentration was determined by Pierce™ BCA Protein Assay Kit (ThermoFisher).
For Western blot analysis, 25 µg of protein per sample was separated by SDS-PAGE and transferred onto a nitrocellulose membrane by semidry blot. For detection of proteins, following antibodies were used: CREB1 antibody 48H2 (CST), Argonaute-2 antibody C34C6 (CST), Maltose Binding Protein antibody ab9084 (Abcam), and β-actin antibody ab8227 (Abcam). As secondary antibody, a horseradish peroxidase conjugated goat α-mouse/rabbit antibody (CST) was used. Chemiluminescent blots were imaged by LAS-3000 Imaging System (Fuji). For densitometric analysis of western blot signal intensity ImageJ software^{51 (link)} was used.

Friedrich M., Heimer N., Stoehr C., Steven A., Wach S., Taubert H., Hartmann A, & Seliger B. (2020). CREB1 is affected by the microRNAs miR-22-3p, miR-26a-5p, miR-27a-3p, and miR-221-3p and correlates with adverse clinicopathological features in renal cell carcinoma. Scientific Reports, 10, 6499.

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Antibody Reagents for Protein Expression

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Unless stated otherwise, all chemical reagents were purchased from either Applichem (Darmstadt, Germany) or Sigma-Aldrich (Munich, Germany), and all cell culture reagents were purchased from either PAA laboratories (Cölbe, Germany) or Biochrom AG (Berlin, Germany). Rabbit polyclonal PDZK1 antibody (PAB15564) was from Abnova (Jhongli City, Taoyuan County, Taiwan), rabbit polyclonal NHE3 antibody (NHE31-A) was from Alpha diagnostic (San Antonio, USA), and rabbit polyclonal β-actin antibody (ab8227) was from Abcam (Cambridge, UK).

Yeruva S., Chodisetti G., Luo M., Chen M., Cinar A., Ludolph L., Lünnemann M., Goldstein J., Singh A.K., Riederer B., Bachmann O., Bleich A., Gereke M., Bruder D., Hagen S., He P., Yun C, & Seidler U. (2014). Evidence for a causal link between adaptor protein PDZK1 downregulation and Na+/H+ exchanger NHE3 dysfunction in human and murine colitis. Pflugers Archiv, 467(8), 1795-1807.

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TLR4 Signaling Pathway Regulation

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TLR4 can regulate TLR inflammatory response via phospho-p65 NF-kappa B p65.35 (link) Mitogen-activated protein kinase (MAPK) as regulatory signal mediators plays a pivotal role in organism immunity.36 (link) To understand the effects of miR-29b on TLR4 signaling, phosphorylated MAPK and NF-kappa B as second messengers in TLR4 signaling37 (link) were measured by Western blot. The antihuman antibodies of TLR4 (ab17942), Phospho-p65 anti-NF-kappa B (ab86299), phospho-p38 mitogen-activated protein kinase (MAPK, ab31828), IL-1 beta (ab2105), IL-6 (ab6672), β-actin antibody (ab8227), and secondary antibody Goat Anti-Rabbit IgG H&L (HRP, ab6721) were purchased from Abcam (Beijing, China). Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk in the buffer (10 mM Tris [pH 7.5] and 50 mM NaCl) and incubated with primary antibodies overnight at 4°C. The membrane was further treated with secondary antibodies (1:3,000). Immunoreactive bands were visualized by enhanced chemiluminescence system (Amersham, Arlington Heights, IL, USA).

Yang Y., Ma Z., Yang G., Wan J., Li G., Du L, & Lu P. (2017). Alginate oligosaccharide indirectly affects toll-like receptor signaling via the inhibition of microRNA-29b in aneurysm patients after endovascular aortic repair. Drug Design, Development and Therapy, 11, 2565-2579.

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Matrine and Baicalin Apoptosis Regulation

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Matrine (SM8130) and baicalin (SB8020) were purchased from Beijing Solarbio Science and Technology Co. Ltd. (Beijing, China); DMEM/F-12 and fetal bovine serum (FBS) were purchased from Gibco; SYBR Premix Ex Taq II (RR820A) and PrimeScript RT Reagent Kit with gDNA Eraser (RR047A) were purchased from Takara Bio Inc. (Shiga, Japan). The LukS-PV antibody (Cat. No. ab190473), cleaved caspase-3 antibody (ab49822), cleaved caspase-8 antibody (ab25901), β-actin antibody (ab8227), and goat anti-rabbit IgG (HRP; ab205718) were purchased from Abcam (Cambridge, UK). Cleaved caspase-9 antibody (Cat. No. 10380-1-AP) was purchased from Proteintech (Rosemont, IL). An annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis kit (Cat. No. KA3805) was purchased from Abnova (Taipei, Taiwan). The live/dead reagent (Cat. No. KGAF001), whole-cell lysis assay kit (KGP2100), and a bicinchoninic acid (BCA) protein assay kit (KGPBCA) were purchased from Keygen Biotech (Nanjing, China). Hoechst 33342 (Cat. No. C0030) was purchased from Solaibao (Beijing, China).

Jia F., Ma W., Zhang X., Wang D, & Zhou X. (2020). Matrine and baicalin inhibit apoptosis induced by Panton-Valentine leukocidin of Staphylococcus aureus in bovine mammary epithelial cells. Journal of dairy science, 103(3).

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Antibody Reagents for Protein Expression

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