Kjeltec 2300 autoanalyser

Manufactured by Foss

Sourced in Sweden

The Kjeltec 2300 Autoanalyser is a laboratory instrument designed for the automated analysis of nitrogen content in various samples. It performs the Kjeldahl method of nitrogen determination, which is a widely used technique in chemical analysis. The Kjeltec 2300 Autoanalyser automates the digestion, distillation, and titration steps of the Kjeldahl method, providing efficient and accurate results.

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Lab products found in correlation

Spad 502 meter, by Konica Minolta (1 mentions) Infratec 1241 grain analyzer, by Foss (1 mentions) Hi 9033, by Hanna Instruments (1 mentions) Hi 9025, by Hanna Instruments (1 mentions) Dionex model dx500, by Thermo Fisher Scientific (1 mentions)

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Kjeltec 2300 (22 citations)

4 protocols using kjeltec 2300 autoanalyser

Soybean Chlorophyll and Nitrogen Analysis

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The chlorophyll in the soybean leaves was determined using samples consisting of five leaf disk obtained using a circular punch (cork borer) on five different leaves. The chlorophyll content was determined using a SPAD-502 meter (Konica-Minolta, Japan).
The roots and shoots were oven dried at 75°C for 2 days and ground. The total nitrogen content in the roots and shoots was determined using the Kjeldahl method with a nitrogen analyser (Kjeltec 2300 Autoanalyser, Foss Tecator AB, Hoganas, Sweden).

Egamberdieva D., Jabborova D., Wirth S.J., Alam P., Alyemeni M.N, & Ahmad P. (2018). Interactive Effects of Nutrients and Bradyrhizobium japonicum on the Growth and Root Architecture of Soybean (Glycine max L.). Frontiers in Microbiology, 9, 1000.

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Grain Quality and Yield Traits Analysis

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Rice grains of each sample were harvested at ~40 DAF and air-dried, then stored at room temperature for 3 months for measuring grain quality and yield traits. For each plant, 15–20 g of brown rice were prepared for the measurement of grain quality, including AC, GC, starch content, and GPC. The GPC was carefully determined by near-infrared spectroscopy using Infratec 1241 Grain Analyzer (Foss Tecator, Sweden) equipped with STM model^{42 (link)}. Briefly, approximately 15–20 g dry seeds per sample were dehulled into brown rice using a TR 200 dehuller (Kett, Tokyo, Japan) and loaded into the sample cup. Three scans per sample were used in data analysis. Data of seed protein contents were presented on a 0% moisture basis. These calibrations involved more than 500 rice samples that varied in GPC. Crude GPC and storage protein fractions in rice flour were also determined by the Kjeldahl method using a Kjeltec 2300 Autoanalyser (Foss AB, Sweden)^{28 (link)}. A nitrogen conversion factor of 6.25 was used to calculate the GPC.

Yang Y., Guo M., Sun S., Zou Y., Yin S., Liu Y., Tang S., Gu M., Yang Z, & Yan C. (2019). Natural variation of OsGluA2 is involved in grain protein content regulation in rice. Nature Communications, 10, 1949.

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Grains Characterization Protocol

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Barley grains were milled to pass through a 1 mm Retsch Muhle mill (Retsch EPP 15×20, Germany), and dried using an air-forced oven at 60°C for 48 h. Nitrogen value was characterized using the Kjeldahl procedure (Kjeltec 2300 Autoanalyser, Foss Tecator AB, Hoganas, Sweden) and CP was estimated as N×6.25. Starch was determined by an anthrone/sulphuric acid method using glucose as standard and estimated as 0.9×glucose content [19 ]. The NDF (measured without alpha amylase and sodium sulphite) and acid detergent fibre (ADF; measured without alpha amylase and sodium sulphite). Samples were also analysed for ether extract and ash concentrations [20 ].

Naseroleslami R., Mesgaran M.D., Tahmasbi A., Vakili S.A, & Ebrahimi S.H. (2017). Influence of barley grain treated with alkaline compounds or organic extracts on ex vivo site and extent of digestion of starch. Asian-Australasian Journal of Animal Sciences, 31(2), 230-236.

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Quantifying Inorganic Ions and Nutrients in Plants

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Inorganic ions (both from NS and plant material) were determined by ion chromatography (Dionex model DX500; Dionex Corporation, Sunnyvale, CA, USA) with a conductivity detector, using the pre-column IonPack AG14 and the column of separation IonPack AS14 for the anions, and the pre-column IonPack CG12A and the column of separation IonPack CS12A for the cations (Di Gioia et al., 2013 ). Ultrapure water at 18 MΩ/cm (Milli-Q Academic Millipore) was used in all the analysis.
Total Kjeldahl nitrogen in the plant was determined from 0.1 g of dried and ground leaf tissue by the Kjeldahl method (Kjeltec 2300 Auto Analyser; Foss-Tecator, Hillerød, Denmark) adding salicylic acid to recover the NO₃-N (15 mL 0.18 M salicylic acid in 96% H₂SO₄, selenium compounds and Zn as catalysts). Plant phosphorus content was determined by spectrophotometry.
The pH and EC of NS were measured using the portable pH-meter HI 9025 and the conductivity-meter HI-9033 (Hanna Instruments, Padova, Italy). The pH was not subjected to analysis because its correction was done only to maintain its value in the 5.5–6.5 range, in the same manner for all the treatments.
Total soluble solids were measured using a portable reflectometer (Brixstix BX 100 H; Techniquip Corporation, Livermore, CA, USA); the DM was determined after drying until constant weight in a forced-draft oven at 65°C.

Signore A., Serio F, & Santamaria P. (2016). A Targeted Management of the Nutrient Solution in a Soilless Tomato Crop According to Plant Needs. Frontiers in Plant Science, 7, 391.

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