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2 protocols using phospho s6rb

1

Signaling Pathway Profiling in Cancer Cells

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TNF-a was purchased from R&D Systems (catalog #210-TA/CF). ON123300 was obtained from Onconova Therapeutics, Inc. PD0332991, U0126 and QNZ were purchased from Selleckchem. Antibodies directed against PARP (catalog #9542), CASPASES 3 (catalog #9665), 7 (catalog #9492) and 9 (catalog #9502), phospho-RbSer780 (catalog #9307), Rb (catalog #9309), phospho-mTORSer2481 (catalog #2974), mTOR (catalog #4517, phospho-4EBP1 (catalog #9459), 4EBP1 (catalog #9644), phospho-S6RB (catalog #2211), S6RB (catalog #2317), phospho-FOXO1 (catalog #9461), phospho-ERK (catalog #4370), ERK (catalog #9107), phospho-MEK (catalog #9154), MEK (catalog #4694), phospho-NF-κB p65Ser536 (catalog #3033), and phospho-RbSer807/811 (catalog #9308) were obtained from Cell Signaling Technologies. GAPDH (catalog #SC-47724), AKT (catalog #SC-1618), p130 (catalog #SC-317), CDK4 (catalog #SC-260), CDK2 (catalog #SC-163), CYCLIN D1 (catalog #SC-8396), NF-κB p65 (catalog #sc-8008) and p21-specific (catalog #SC-756) antisera were purchased from Santa Cruz Biotechnology. The phospho-AKTSer473 antibody was purchased from R&D Systems (catalog #AF-887). Phospho-p130 antiserum was obtained from Abcam (catalog #AB68136).
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2

Signaling Pathway Profiling in Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF-a was purchased from R&D Systems (catalog #210-TA/CF). ON123300 was obtained from Onconova Therapeutics, Inc. PD0332991, U0126 and QNZ were purchased from Selleckchem. Antibodies directed against PARP (catalog #9542), CASPASES 3 (catalog #9665), 7 (catalog #9492) and 9 (catalog #9502), phospho-RbSer780 (catalog #9307), Rb (catalog #9309), phospho-mTORSer2481 (catalog #2974), mTOR (catalog #4517, phospho-4EBP1 (catalog #9459), 4EBP1 (catalog #9644), phospho-S6RB (catalog #2211), S6RB (catalog #2317), phospho-FOXO1 (catalog #9461), phospho-ERK (catalog #4370), ERK (catalog #9107), phospho-MEK (catalog #9154), MEK (catalog #4694), phospho-NF-κB p65Ser536 (catalog #3033), and phospho-RbSer807/811 (catalog #9308) were obtained from Cell Signaling Technologies. GAPDH (catalog #SC-47724), AKT (catalog #SC-1618), p130 (catalog #SC-317), CDK4 (catalog #SC-260), CDK2 (catalog #SC-163), CYCLIN D1 (catalog #SC-8396), NF-κB p65 (catalog #sc-8008) and p21-specific (catalog #SC-756) antisera were purchased from Santa Cruz Biotechnology. The phospho-AKTSer473 antibody was purchased from R&D Systems (catalog #AF-887). Phospho-p130 antiserum was obtained from Abcam (catalog #AB68136).
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