Rabbit anti fibrillarin

Manufactured by Abcam

Sourced in United Kingdom

Rabbit anti-Fibrillarin is a primary antibody that specifically recognizes the nucleolar protein fibrillarin. Fibrillarin is a highly conserved 34-kDa nucleolar protein that plays a role in the processing of pre-rRNA and the formation of small nucleolar ribonucleoproteins (snoRNPs).

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Lab products found in correlation

Axioplan microscope, by Zeiss (2 mentions) Mouse anti gfp, by Roche (1 mentions) Sp2 confocal microscope, by Leica camera (1 mentions) Rabbit anti ha, by Fortis Life Sciences (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Mouse anti v5, by Thermo Fisher Scientific (1 mentions) Mouse anti sycp3, by Santa Cruz Biotechnology (1 mentions) Fitc conjugated goat anti mouse igg, by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Texas red conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Goat anti lamin b, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 647, by Abcam (1 mentions) Vectashield vibrance antifade mounting medium with dapi, by Vector Laboratories (1 mentions) Guinea pig anti p62 sqstm1, by Progen Biotechnik (1 mentions) Hrp conjugated anti rabbit, by Merck Group (1 mentions) Anti mouse, by Merck Group (1 mentions) Rabbit anti mda5, by Cell Signaling Technology (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti rig 1, by Cell Signaling Technology (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)

5 protocols using rabbit anti fibrillarin

Hoechst Squash and Immunofluorescence Staining

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For live phase/Hoechst squash, dissected testes were cut open on a slide in 70 μl of PBS containing 2μg/ml Hoechst 33342, gently squashed by lowering a coverslip and wicking out some solution, and examined immediately on a Zeiss Axioplan microscope. Images were taken with a Photometrics COOLSNAP EZ camera and processed with Adobe Photoshop software.
Immunofluorescence was performed as previously described [15 (link)], using the following antibodies: mouse anti-GFP (Roche), rabbit anti-HA (Bethyl Laboratories), mouse anti-V5 (Invitrogen), mouse anti-MYC (Millipore), anti-Aly [22 (link)], anti-Topi [20 (link)], mouse anti-Fibrillarin (Cytoskeleton), rabbit anti-Fibrillarin (Abcam) and anti-Med26 [37 (link)]. Alex-fluor-conjugated goat secondary antibodies were used. Images were captured with either a Leica SP2 confocal microscope or a Leica SP5 confocol microscope and processed with Adobe Photoshop software.

Lu C, & Fuller M.T. (2015). Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage. PLoS Genetics, 11(12), e1005701.

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Immunofluorescence Staining of SYCP3 and Fibrillarin

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The slides were incubated for 45 minutes at 37 °C in a moist chamber with the primary antibodies: mouse anti-SYCP3 1:100 (Santa Cruz Biotechnology, ab74569) and rabbit anti-Fibrillarin 1:100 (Abcam, Cambridge, UK, ab5821). Then, the slides were incubated for 30 min at room temperature with the secondary antibodies: FITC-conjugated goat anti-mouse IgG (1:50) (Sigma), or Texas red-conjugated goat anti rabbit IgG (1:200) (Jackson ImmunoResearch). Slides were counterstained with 1 μg/mL DAPI (4′, 6-diamidino-2-phenylindole). Finally, slides were rinsed in PBS and mounted in Vectashield (Vector Laboratories).

López-Moncada F., Tapia D., Zuñiga N., Ayarza E., López-Fenner J., Redi C.A, & Berríos S. (2019). Nucleolar Expression and Chromosomal Associations in Robertsonian Spermatocytes of Mus musculus domesticus. Genes, 10(2), 120.

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Immunofluorescence Staining of Cultured Cells

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Cultured cells (neurons and MEFs) were washed with PBS and fixed for 15 min in 4% paraformaldehyde in PBS. After fixation, cells were washed with PBS and incubated for 1 h in blocking solution containing 10% FCS and 0.2% Triton X-100 in PBS. Cells were then incubated overnight at 4 °C in blocking solution containing primary antibody (1:50): goat anti-lamin B (catalog no. sc-6216; Santa Cruz Biotechnology); rabbit anti-LC3; guinea pig anti-p62/SQSTM1 (catalog no. GP62-C; Progen); rabbit anti-Syne-1 or rabbit anti-fibrillarin (catalog no. ab5821; Abcam). Coverslips were then washed with PBS, followed by secondary antibody staining for 1 h at room temperature at 1:500 dilution: anti-goat Alexa Fluor 555 (catalog no. ab150134; Abcam); anti-rabbit Alexa Fluor 488 (catalog no. ab150073; Abcam); or goat anti-guinea pig Alexa Fluor 647 (catalog no. ab150187; Abcam). VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (catalog no. H-1800; Vector Laboratories) was used to mount coverslips on slides and stain nuclei. Confocal images of fluorescently labeled proteins were captured using a 40× objective lens on an LSM 710 NL multi-photon confocal microscope (ZEISS). Puncta number, size and pixel intensity measurements were performed with ImageJ after background subtraction and threshold setting.

Papandreou M.E., Konstantinidis G, & Tavernarakis N. (2022). Nucleophagy delays aging and preserves germline immortality. Nature Aging, 3(1), 34-46.

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Western Blotting of Innate Immune Sensors

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Cells (1–1.2 × 10⁶) were lysed in 50 mM Tris-HCl pH 8, 150 mM NaCl, 1% Triton X-100, and 1 mM EDTA with the addition of protease inhibitors (cOmplete, Roche). Protein samples were separated at 100 V by 10% SDS-PAGE electrophoresis and blotted on a nitrocellulose membrane. Membranes were incubated with specific antibodies: mouse anti-α-tubulin (1:8000, Sigma-Aldrich), rabbit anti-MDA5 (1:800, Cell Signaling), rabbit anti-RIGI (1:800, Cell Signaling), rabbit anti-fibrillarin (1:10,000, Abcam). HRP-conjugated anti-rabbit (1:80,000) and anti-mouse (1:5000) antibodies were purchased from Sigma-Aldrich. Proteins were revealed with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). All immunoblots blots were performed in triplicate and the quantification of the protein bands from all three experiments is shown in each figure.

Wiatrek D.M., Candela M.E., Sedmík J., Oppelt J., Keegan L.P, & O'Connell M.A. (2019). Activation of innate immunity by mitochondrial dsRNA in mouse cells lacking p53 protein. RNA, 25(6), 713-726.

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Immunohistochemistry of Drosophila Imaginal Discs

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Eye-antennal imaginal discs and salivary glands were prepared for immunohistochemistry using standard protocols. As the growth conditions strongly affect salivary gland size, all the experiments were controlled by synchronization of L3 wandering larvae after a single-day egg collection. To further control this issue, a controlled the egg laying for 5 h was set up, and the salivary glands were analysed 96 h after egg laying (96–101 h AEL; electronic supplementary material, figure S4g–j′).
Primary antibodies used were: mouse anti-Armadillo N27A1 at 1 : 100 (Developmental Studies Hybridoma Bank, DSHB), mouse anti-Dlg at 1 : 1000 (4F3, DSHB), rabbit anti-Viriato (Vito) at 1 : 250 (ABGent), rat anti-DCad at 1 : 100, mouse anti-AH6 at 1 : 10 (DSHB), rabbit anti-Fibrillarin at 1 : 250 (Abcam, #ab5821), mouse anti-Fibrillarin at 1 : 500 (Abcam, #ab4566), mouse anti-RpS6 at 1 : 100 (Cell Signaling, #2317), mouse anti-RpL11 at 1 : 100 (Abcam, #ab79352), mouse anti-RpL10A at 1 : 400 (Abcam, #ab55544), rabbit anti-RpL22 at 1 : 100 (kind gift from Dr Vassie Ware). To stain for cellular limits phalloidin conjugated with rhodamine was used at a dilution of 1 : 1000. Appropriate Alexa-Fluor conjugated secondary antibodies were from Molecular Probes. Images were obtained with the Leica SP2 confocal system or Leica SP5 confocal system and processed with Adobe Photoshop.

Martins T., Eusebio N., Correia A., Marinho J., Casares F, & Pereira P.S. (2017). TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands. Open Biology, 7(1), 160258.

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