Cis diltiazem hydrochloride

Native human AAG (lot no. SLBJ6840V), carvedilol, (+)-cis-diltiazem hydrochloride, dipyridamole, vinblastine sulfate, (±)-propranolol hydrochloride, imipramine hydrochloride, lidocaine, and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). All standards had a specified purity of ≥97% and have been used without further purification. Sodium phosphate dihydrate and sodium hydroxide were purchased from Kemika (Zagreb, Croatia). Tween^® 20 was purchased from Fischer Scientific (Pittsburgh, PA, USA). Guanidine hydrochloride was purchased from AppliChem GmbH (Darmstadt, Germany). Ultrapure water was obtained using SG Ultra Clear UV Plus (SG, Burladingen, Germany). All other chemicals used in the study were of analytical grade or higher purity.

Theophylline, dextromethorphan hydrobromide, diclofenac sodium salt, chlorzoxazone, (+)-cis-diltiazem hydrochloride, NADPH, and heparin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methoxyresorufin, p-nitrophenol, resorufin, testosterone, and 4-nitrocatechol were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dextrophan, 6-hydroxychlorzoxazone, 4-hydroxydiclofenac, and 6-β-hydroxytestosterone were purchased from Ultrafine Chemicals (Manchester, UK). All other reagents and chemicals were of analytical grade and were obtained commercially.

The reagents that were used in experiments are listed below: Paraformaldehyde (P6148), Poly(ethyleneimine) solution (P3143), penicillin–streptomycin (P4333), (+)-cis-Diltiazem hydrochloride (D2521), (±)-Verapamil hydrochloride (V4629), Isradipine (I6658), Nifedipine (N7634) (Sigma-Aldrich, Saint Louis, MO, USA), Neurobasal-A medium (10888022), B-27 supplement (17504044), Trypsin 2.5% (15090046), Goat serum New Zeland origin (16210072) (Life Technologies, Grand Island, NY, USA), Fura-2 AM (F1221), Hoechst 33,342 Trihydrochloride Trihydrate (H1399) (Molecular Probes, Eugene, OR, USA), ML 218 hydrochloride (4507) (Tocris Bioscience, Bristol, UK), NBQX disodium salt (N-186), D-AP5 (D-145) (Alomone Labs, Jerusalem, Israel), Bicuculline (11727) (Cayman Chemical, Ann Arbor, MI, USA), goat anti-mouse Alexa Fluor 647 antibody (ab150115) (Abcam, Cambridge, UK), monoclonal mouse anti-GFAP antibodies (GF1 clone, L18/03) (Bialexa, Moscow, Russian Federation); Triton X-100 (Am-O694) (Amresco LLC, Solon, OH, USA); EGTA (A-0878), EDTA (A5097), (AppliChem, Darmstadt, Germany); HEPES (Cat. No 3350) (Dia-M, Moscow, Russian Federation).