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Nebnext chip seq library prep

Manufactured by New England Biolabs
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Sourced in Germany, United States

The NEBNext Chip-Seq Library Prep kit is a tool for preparing DNA libraries for chromatin immunoprecipitation sequencing (ChIP-seq) applications. The kit provides the necessary reagents and protocols to convert immunoprecipitated DNA fragments into sequencing-ready libraries.

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Lab products found in correlation

Smarter ultra low rna kit, by Illumina (2 mentions) Hiseq 2000, by Illumina (2 mentions)

2 protocols using nebnext chip seq library prep

1

RNA-Seq Library Generation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Full-length double-stranded cDNA was generated from 5 ng of total RNA and amplified using the SMARTer Ultra Low RNA Kit (Illumina, San Diego, CA, USA). Library preparation was performed from 10 ng of fragmented cDNA using the NEBNext Chip-Seq Library Prep protocol (New England BioLabs, Frankfurt am Main, Germany). Libraries were sequenced on an Illumina Hiseq2000 with 2 × 50-bp paired-end reads (n = 152).
Schmitt M., Hückelhoven A.G., Hundemer M., Schmitt A., Lipp S., Emde M., Salwender H., Hänel M., Weisel K., Bertsch U., Dürig J., Ho A.D., Blau I.W., Goldschmidt H., Seckinger A, & Hose D. (2016). Frequency of expression and generation of T-cell responses against antigens on multiple myeloma cells in patients included in the GMMG-MM5 trial. Oncotarget, 8(49), 84847-84862.
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2

Transcriptome Profiling by RNA-Sequencing

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA-sequencing was performed as published (12 (link), 16 (link), 17 (link)). In brief, full-length double-stranded cDNA was generated from 5 ng of total RNA and amplified using the SMARTer Ultra Low RNA Kit (Illumina, San Diego, CA, USA). Library preparation was performed from 10 ng of fragmented cDNA using the NEBNext Chip-Seq Library Prep protocol (New England BioLabs, Ipswich, MA, USA). Libraries were sequenced on an Illumina Hiseq2000 with 2 × 50-bp paired-end reads.
Seckinger A., Hillengass J., Emde M., Beck S., Kimmich C., Dittrich T., Hundemer M., Jauch A., Hegenbart U., Raab M.S., Ho A.D., Schönland S, & Hose D. (2018). CD38 as Immunotherapeutic Target in Light Chain Amyloidosis and Multiple Myeloma—Association With Molecular Entities, Risk, Survival, and Mechanisms of Upfront Resistance. Frontiers in Immunology, 9, 1676.
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