GES-1 is a SV40 transformed human fetal gastric epithelial cell line, which were established in 1994 and proved to maintain a normal cytoskeleton, positive in PAS reaction and were non-tumourigenic in nude mice.30 (link) GES-1, SGC7901, MKN28, MKN45 and AGS cells (originally purchased from ATCC) were maintained in RPMI-1640 medium; BGC823 and HEK293T cells (originally purchased from ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Thermo Scientific HyClone, Beijing, China).
Dulbecco s modified eagle s medium
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Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium formulated to support the growth and maintenance of various cell types. It provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell proliferation and survival. DMEM is a widely used medium in cell biology research and applications.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3113 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (984 mentions)
Penicillin, by Thermo Fisher Scientific (726 mentions)
Streptomycin, by Thermo Fisher Scientific (711 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (419 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (400 mentions)
L glutamine, by Thermo Fisher Scientific (234 mentions)
Rpmi 1640, by Thermo Fisher Scientific (205 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (167 mentions)
Fetal bovine serum (fbs), by Merck Group (166 mentions)
Trypsin edta, by Thermo Fisher Scientific (136 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (123 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (116 mentions)
Trypsin, by Thermo Fisher Scientific (114 mentions)
Penicillin, by Merck Group (106 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (104 mentions)
Glutamax, by Thermo Fisher Scientific (101 mentions)
Hek293t, by American Type Culture Collection (101 mentions)
Streptomycin, by Merck Group (99 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (88 mentions)
6 078 protocols using dulbecco s modified eagle s medium
1
Cell Lines for Gastric Cancer Research
2
Culturing Human Colon Cancer Cells
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Human colon cancer cell line was used in the present study. SNU-C2A cells were purchased from Korean Cell Line Bank (Seoul, Korea) and cultured in Dulbecco’s modified Eagle’s medium (Thermo Scientific HyClone, Logan, UT, USA) supplemented with 10% (v/v) fetal bovine serum and penicillin (100 U/mL)-streptomycin (100 μg/mL) obtained from GIBCO/BRL Life Technologies (Grand Island, NY, USA). The cells were maintained in a humidified incubator at 37°C in an atmosphere containing 5% CO2 and 95% air.
3
Clinical Gastric Cancer Tissue Collection and Cell Culture Protocol
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We collected GC tissues from GC patients who had undergone operation at Nanjing Drum Tower Hospital, China. The clinicopathologic characteristics of these patients are described in Supplemental Table 1
4
Gastric Cancer Cell Lines and Tissue Samples
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The human GC cell lines SGC7901, BGC823, MKN45, and AGS, and the immortalized gastric epithelial cell line GES were purchased from the Cell Resource Center of the Chinese Academy of Sciences, Shanghai, China. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (Thermo Scientific HyClone, Beijing, China) supplemented with 10% fetal bovine serum (HyClone), 100 U/mL penicillin, and 100 U/mL streptomycin (HyClone) in a 37 °C humidified incubator with a mixture of 95% air and 5% CO2.
A total of 20 fresh primary GC samples and matched adjacent non-cancerous tissues were obtained from patients undergoing surgery at Xijing Hospital, Xi’an, China. All samples were confirmed by the Department of Pathology at Xijing Hospital and stored in a liquid nitrogen canister. All patients provided informed consent for excess specimens to be used for research purposes and all protocols employed in the present study were approved by the Medical Ethics Committee of Xijing Hospital.
A total of 20 fresh primary GC samples and matched adjacent non-cancerous tissues were obtained from patients undergoing surgery at Xijing Hospital, Xi’an, China. All samples were confirmed by the Department of Pathology at Xijing Hospital and stored in a liquid nitrogen canister. All patients provided informed consent for excess specimens to be used for research purposes and all protocols employed in the present study were approved by the Medical Ethics Committee of Xijing Hospital.
5
Culturing HCC and Kidney Cell Lines
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Human HCC cell lines (MHCC-LM3, MHCC-97H, MHCC-97L, SMMC-7721, and HepG2), the normal human hepatic cell line LO2, and human embryonic kidney cell lines (HEK293T)were grown in Dulbecco’s modified Eagle’s medium (Thermo Scientific Hyclone, Rockford, IL, USA) that was supplemented with 10% fetal bovine serum (Life Technologies, Inc. Rockville, MD, USA) and 1% penicillin/streptomycin (Life Technologies) in a humidified atmosphere of 5% CO2 at 37 °C.
6
Culturing RAW 264.7 Macrophage-Osteoclast
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The mouse macrophage cell line RAW 264.7, a well-established osteoclastogenic cell system that differentiate into TRAP-positive functional osteoclasts when co-cultured with RANKL [35] (link), was purchased from American Type Culture Collection (ATCC, USA). RAW 264.7 cells were maintained in Dulbecco's modified Eagle's medium (Thermo Scientific HyClone, USA) supplemented with 10% fetal bovine serum (Thermo Scientific HyClone, USA) and antibiotics (100 U/ml penicillin G and 100 µg/ml streptomycin) at 37°C with 5% CO2.
7
Culturing Lung Cancer and Endothelial Cells
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The human lung adenocarcinoma cells A549 and Large-cell lung cancer cell line H460 (ATCC; Manassas, VA, USA) were purchased from Shanghai cell bank of Chinese academy of sciences. These cell lines were cultured in Dulbecco’s modified Eagle’s medium (Thermo Scientific Hyclone, South Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Life Technologies, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Hyclone). Human umbilical vein endothelial cells (HUVECs) and its completed medium were obtained from AllCells biological technology (Shanghai) co., LTD. And all these cell lines were maintained in a 37 °C incubator with 5% CO2.
8
Isolation of Mesenteric Cells
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The mesenteric tissue was cut into 1 × 1 mm tissue fragments with sterile surgical scissors and digested in Dulbecco’s modified Eagle’s medium (Invitrogen) containing 1.5 mg·mL−1 type II collagenase (C6885; Sigma‐Aldrich, St Louis, MO, USA) and 60 U·mL−1 type I DNase (D5025; Sigma‐Aldrich). After 2 h of incubation on a table concentrator (60 r.p.m.) at 37 °C, digestion was stopped with ice‐cold Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Invitrogen) and filtered through a 70‐μm filter. The cell mixture was then centrifuged at 300 g for 10 min at 4 °C, and the supernatant was removed. Finally, the cell pellet was resuspended in 5 mL of complete medium and seeded.
9
Porcine Kidney Cell-Based Antiviral Protocol
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LLC-PK1 cells (porcine kidney cells) were purchased from the ATCC (ATCC CL-101) and cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA) supplemented with 10% fetal bovine serum at 37 °C in a humidified 5% CO2 incubator. HEK-293T cells, obtained from the China Center for Type Culture Collection (CCTCC), were cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA) supplemented with 10% fetal bovine serum under the same conditions described above. PDCoV strain CHN-HN-2014 (GenBank accession number KT336560) was isolated from a piglet with acute diarrhea in China in 2014 (Dong et al., 2016 (link)). Sendai virus (SEV) was obtained from the Centre of Virus Resource and Information, Wuhan Institute of Virology, Chinese Academy of Sciences. Mouse monoclonal antibodies (MAbs) against hemagglutinin (HA) and β-actin were purchased from Medical and Biological Laboratories (Japan). Rabbit polyclonal antibodies directed against IRF3, phosphorylated IRF3 (p-IRF3), p65, and phosphorylated p65 (p-p65) were purchased from ABclonal (China) and Cell Signaling Technology.
10
Isolation and Maintenance of Primary Mouse and Human Mesenchymal Stem Cells
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Primary mBMSCs were from the femoral bone marrow of wild-type C57BL/6J mice at 3 months of age as previously described.41 (link) The isolated cells were maintained in Dulbecco’s modified Eagle’s medium, containing 15% heat-inactivated fetal bovine serum, 100 U·mL−1 of penicillin and 100 µg·mL−1 of streptomycin sulfate for 3 days (all from Invitrogen). The nonadherent hematopoietic cells were then removed by vigorous washing with phosphate-buffered saline (PBS) three times. The growing colonies were collected by trypsinization after 2 weeks for further passaging and differentiation. The in vivo and in vitro animal procedures were approved by the Committee for the Care and Use of Laboratory Animals of the State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University.
hMSCs were purchased from the ATCC and were maintained in Dulbecco’s modified Eagle’s medium supplemented with 15% FBS plus 100 U·mL−1 penicillin and 100 µg·mL−1 streptomycin sulfate (all from Invitrogen).
hMSCs were purchased from the ATCC and were maintained in Dulbecco’s modified Eagle’s medium supplemented with 15% FBS plus 100 U·mL−1 penicillin and 100 µg·mL−1 streptomycin sulfate (all from Invitrogen).
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