Laquatwin b 712

Manufactured by Horiba

Sourced in Japan

The LAQUAtwin B-712 is a compact and waterproof pH meter designed for on-site water quality measurements. It features an easy-to-read digital display and automatic temperature compensation. The device is powered by a CR2032 lithium battery and comes with a carrying case for portability.

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Lab products found in correlation

Gc fid, by Agilent Technologies (1 mentions) Gc 14a, by Shimadzu (1 mentions) Lc 20a, by Shimadzu (1 mentions) Toc 5000, by Shimadzu (1 mentions) Gc 14b, by Shimadzu (1 mentions) Gc 14b gas chromatograph, by Shimadzu (1 mentions) Arvo mx, by PerkinElmer (1 mentions)

6 protocols using laquatwin b 712

Fecal Biomarker Profiling Protocol

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Fecal pH was measured with an electrode-fitted pH meter LAQUAtwinB-712 (HORIBA, Kyoto, Japan) after suspending fecal samples in Milli-Q water and sterilizing at 85 °C for 15 min for sterilization of fecal samples.
Fecal SCFAs (acetate, propionate, n-butyrate, iso-butyrate, n-valerate, iso-valerate, n-caproic acid) in ethyl acetate extract were determined by gas chromatography system GC-FID (7890B, Agilent Technologies, Santa Clara, CA, USA) and a DB-WAXetr column (30 m, 0.25 mm id, 0.25 μm film thickness, 1.2 mL/min) as described previously [42 (link)].
Commercially available ELISA kits were used to determine fecal secretory immunoglobulin A (sIgA: Human IgA ELISA Core Kit, LABISKOMA, Seoul, Republic of Korea) and calprotectin (IDK^® Calprotectin MRP 8/14 ELISA kit, Immundiagnostik AG, Bensheim, Germany).

Hiraku A., Nakata S., Murata M., Xu C., Mutoh N., Arai S., Odamaki T., Iwabuchi N., Tanaka M., Tsuno T, & Nakamura M. (2023). Early Probiotic Supplementation of Healthy Term Infants with Bifidobacterium longum subsp. infantis M-63 Is Safe and Leads to the Development of Bifidobacterium-Predominant Gut Microbiota: A Double-Blind, Placebo-Controlled Trial. Nutrients, 15(6), 1402.

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Biogas Composition and Organic Acid Analysis

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Methane, H₂, and CO₂ in the biogas were measured using a gas chromatograph (GC-14A; Shimadzu, Tokyo, Japan) equipped with a thermal conductivity detector (TCD). The total chemical oxygen demands (COD) of the medium and effluent were determined using a dichromate method according to the Japanese Industrial Standard (JIS) K-1012. The amount of acetate, propionate, and some other organic acids in fermentation liquid were measured using a high-performance liquid chromatography organic acid analysis system (LC-20A, Shimadzu) according to the manufacturer’s instructions. The pH of the fermentation liquid was monitored using a pH meter (LAQUA twin B-712, Horiba, Tokyo, Japan). The protein content of PF and BF was determined using the B-PER II bacterial protein extraction reagent (Pierce, Rockford, IL, USA) and Micro bicinchoninic acid (BCA) protein assay kit (Pierce) according to the manufacturer’s instructions.

Kouzuma A., Tsutsumi M., Ishii S., Ueno Y., Abe T, & Watanabe K. (2017). Non-autotrophic methanogens dominate in anaerobic digesters. Scientific Reports, 7, 1510.

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Sow Urine pH Monitoring for Metabolic Acidosis

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To determine whether metabolic acidosis was being achieved in response to the different DCAD diets prior to farrowing, sow urine pH was measured. Urine was collected at 0700 hours daily from individual pregnant sows in the farrowing house, ~30 min before the morning feed. From 2 d post entry to the farrowing house, all sows were encouraged to stand, and ~20 mL of urine was collected mid-stream from any sows that subsequently urinated. Once a sow had two daily urine samples collected prior to farrowing, no further attempts were made to collect a daily sample. Urine samples were immediately tested for pH using a handheld pH meter (LAQUAtwin B-712, HORIBA Ltd., Kyoto, Japan). Statistical analysis was conducted on samples obtained from 171 sows on the day before farrowing. From 10 d postfarrowing, daily urine samples were collected from as many sows as possible using the same method as described above.

Weaver A.C., Braun T.C., Braun J.A., Golder H.M., Block E, & Lean I.J. (2024). Effects of negative dietary cation–anion difference and calcidiol supplementation in transition diets fed to sows on piglet survival, piglet weight, and sow metabolism. Journal of Animal Science, 102, skae027.

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Analyzing Organic and Nutritional Content

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Total organic carbon (TOC) was determined using a TOC analyzer (TOC-5000, Shimadzu). Total solids (TS); volatile solids (VS); total Kjeldahl nitrogen (TKN); and pH were measured using standard methods [20 ].
The salinity was calculated from the standard curve of conductivity and the concentration of NaCl [21 (link)]. pH was measured by using a pH meter (LAQUAtwin B-712; Horiba, Kyoto, Japan). All tests were conducted in triplicate, and average values are presented in Table 1.

Abouelenien F., Miura T., Nakashimada Y., Elleboudy N.S., Al-Harbi M.S., Ali E.F, & Shukry M. (2021). Optimization of Biomethane Production via Fermentation of Chicken Manure Using Marine Sediment: A Modeling Approach Using Response Surface Methodology. International Journal of Environmental Research and Public Health, 18(22), 11988.

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Rumen Fluid Analysis: pH, VFA, and Ammonia

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The rumen fluid samples were subjected to pH measurement using a pH electrode (LAQUAtwin B‐712; Horiba, Kyoto, Japan) and VFA measurement using a gas chromatograph (GC‐14B; Shimadzu, Kyoto, Japan), as described previously (Oh et al., 2017 (link)). NH₃–N levels in the rumen samples were measured using the phenol–hypochlorite method (Weatherburn, 1967 (link)).

Miura H., Hashimoto T., Kawanishi Y., Kawauchi H., Inoue R., Shoji N., Saito K., Sekiya M., Saito Y., Yasuda J., Yonezawa C., Endo T., Kasuya H., Suzuki Y., Kobayashi Y, & Koike S. (2021). Identification of the core rumen bacterial taxa and their population dynamics during the fattening period in Japanese Black cattle. Animal Science Journal = Nihon Chikusan Gakkaiho, 92(1), e13601.

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Fecal Metabolite Analysis Protocol

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Fecal samples (0.1 g each) were suspended in 500 μL of 0.9% NaCl solution and vortexed, the mixture was centrifuged (4°C, 16,000 × g, 5 min), and the supernatant was used for analysis of pH, VFA, lactate, and ammonia nitrogen (NH 3 -N). Fecal pH was measured using a portable pH meter (LAQUAtwin B-712, Horiba). Volatile fatty acids were measured as described previously (Oh et al., 2017) (link). In brief, the fecal suspension was mixed with 25% metaphosphoric acid at a 5:1 ratio, incubated overnight at 4°C, and centrifuged at 10,000 × g at 4°C for 5 min. Then the supernatant, mixed with crotonic acid as internal standard, was injected into a GC-14B gas chromatograph (Shimadzu) equipped with an UL-BON HR-20M fused silica capillary column (0.53-mm internal diameter × 30-m length, 3.0-μm film; Shinwa) and a flame-ionization detector. The levels of d-and l-lactic acid were measured using a commercial assay kit (Megazyme International Ireland) according to the manufacturer's instructions. Ammonia nitrogen levels were measured via the phenol-hypochlorite reaction method (Weatherburn, 1967) (link) using a microplate reader at 660 nm (Arvo MX, Perkin Elmer).

Kodithuwakku K.A.H.T., Owada H., Miura H., Maruyama D., Hirano K., Suzuki Y., Kobayashi Y, & Koike S. (2021). Effects of oral administration of timothy hay and psyllium on the growth performance and fecal microbiota of preweaning calves. Journal of dairy science, 104(12).

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