C2h5ns

Stannous chloride (SnCl₄·5H₂O, AR, purity 99.0%, Sigma-Aldrich, St. Louis, MO, USA) and thioacetamide (C₂H₅NS, AR, purity ≥ 99%, Sigma-Aldrich, St. Louis, MO, USA) were used as Sn and S precursors, respectively. Trisodium citrate (Na₃C₆H₅O₇, AR, purity 99.9%, Sigma-Aldrich, St. Louis, MO, USA) was used as chelating agent. These compounds were utilized as supplied without additional purification and stored in a humidity-controlled desiccator.

All chemicals used were of analytical grade and were prepared with ultra-high quality deionized water. Bovine Serum Albumin, BSA, (Sigma-Aldrich, www.sigmaaldrich.com, St. Louis. MI, USA). Aqueous solutions of the following reagents, prepared by direct weighing of the species indicated and dissolution in water until the desired concentration was reached: Cd(ClO₄)₂·6H₂O and Thioacetamide, C₂H₅NS, 99% (Sigma Aldrich, Darmstadt, Germany), NaOH, KCl, KH₂PO₄ and Quinine sulfate (2:1) (salt) dihydrate (Scharlab, Barcelona, Spain): 10⁻⁵ mol/L solution in 0.5 H₂SO₄, NaCl (Panreac, Barcelona, Spain), Na₂HPO₄·12H₂O (Prolabo, Barcelona, Spain, Mouse Monoclonal Antibody Anti-GST (vial 250 µg) Immunostep, SL (Salamanca, Spain). Human IVTT reagents from ThermoFischerScientific. (Waltham, MA, USA).

The analytical reagents employed in this study were tin (II) chloride (SnCl₂·2H₂O, Sigma-Aldrich), thioacetamide (C₂H₅NS, Sigma-Aldrich, St. Louis, MO, USA), ethylenediaminetetraacetic acid (EDTA, C₁₀H₁₆N₂O_8, Sigma-Aldrich, St. Louis, USA), and acetone (C₃H₆O, Sigma-Aldrich, St. Louis, USA), used as received without further purification and stored in a humidity-controlled desiccator cabinet. For the preparation of π-SnS nanoparticles, SnCl₂·2H₂O and C₂H₅NS were used as Sn and S sources, respectively, and C₁₀H₁₆N₂O₈ was used as a chelating agent.