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Ad cmv b gal

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Ad-CMV-b-Gal is an adenoviral vector that carries the beta-galactosidase (b-Gal) gene under the control of the Cytomegalovirus (CMV) promoter. It is commonly used as a reporter gene for monitoring viral transduction efficiency in cell-based assays.

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Lab products found in correlation

Ad cmv null, by Vector Biolabs (2 mentions) Ad cmv icre, by Vector Biolabs (2 mentions) Egm 2 medium, by Lonza (1 mentions)

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Ad-gal (9 citations)

4 protocols using ad cmv b gal

1

Lentiviral Transduction of Murine Lung Cells

Cited 2 times
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LLC, E0771, and MMTV-PyMT parental cells were maintained as previously described4 ,24 (link),46 (link). GFP and luciferase were cloned into the pCDH-puro plasmid. The lentiviral vector pLX311-luciferase was a gift from William Hahn (Addgene plasmid #117735). Parental cells were transduced with pCDH-GFP-luc or pLX311-luc. GFP+ cells were enriched using cell sorting. Murine pulmonary microvascular endothelial cells (MPMECs) were isolated from 8 to 16-week-old Rptorfl/fl or Tsc2fl/fl mice and maintained in EGM-2 medium (Lonza), as previously described71 (link)–75 (link). For adenoviral Cre expression, MPMECs were seeded onto tissue culture plates coated with 0.1% gelatin at 70-80% confluency. Cells were infected with 107 PFU ml−1 of Ad-CMV-iCre (Vector Biolabs, #1045) for 16-48 hours, as indicated. Ad-CMV-b-Gal (Vector Biolabs #1080) or Ad-CMV-Null (Vector Biolabs #1300) were used as control vectors as indicated. Cells were harvested 48-72 hours after transduction.
Edwards D.N., Wang S., Song W., Kim L.C., Ngwa V.M., Hwang Y., Ess K.C., Boothby M.R, & Chen J. (2024). Regulation of fatty acid delivery to metastases by tumor endothelium. bioRxiv.
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2

Adenoviral Transduction of Mouse Cells

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Empty adenovirus (Ad-CMV-Null; #1300) and adenoviruses expressing β-Galactosidase (Ad-CMV-b-Gal; #1080) or Cre recombinase (Ad-CMV-iCre; #1045) were from Vector Biolabs. Adenoviral expression vectors for hMfn2 HR1 and the minipeptides and their Gly or Pro mutants were generated and amplified using standard methods. Adenoviral vectors were added to MEFs at 50% confluence at an MOI of 100. Adeno-PA-GFP (mitoGFP) and adeno-mitoDsRed2 (both from SignaGen Laboratories) were added to cultured mouse neurons at an MOI of 10 or 50.
Franco A., Kitsis R.N., Fleischer J.A., Gavathiotis E., Kornfeld O.S., Gong G., Biris N., Benz A., Qvit N., Donnelly S.K., Chen Y., Mennerick S., Hodgson L., Mochly-Rosen D, & Dorn GW I.I. (2016). Correcting mitochondrial fusion by manipulating mitofusin conformations. Nature, 540(7631), 74-79.
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3

Generating MFN2 Mutants and FRET Probes

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MFN2 mutants and FRET probes were generated using PCR mutagenesis, sequence verified, and sent to Vector BioLabs for custom adenovirus production. Ad-CMV-β-Gal was purchased from Vector BioLabs (Cat# 1080).
Franco A., Li J., Kelly D.P., Hershberger R.E., Marian A.J., Lewis R.M., Song M., Dang X., Schmidt A.D., Mathyer M.E., Edwards J.R., Strong C.D, & Dorn G.W. (2023). A human mitofusin 2 mutation can cause mitophagic cardiomyopathy. eLife, 12, e84235.
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4

Generating MFN2 Mutants and FRET Probes

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MFN2 mutants and FRET probes were generated using PCR mutagenesis, sequence verified, and sent to Vector BioLabs for custom adenovirus production. Ad-CMV-β-Gal was purchased from Vector BioLabs (Cat# 1080).
Franco A., Li J., Kelly D.P., Hershberger R.E., Marian A.J., Lewis R.M., Song M., Dang X., Schmidt A.D., Mathyer M.E., Edwards J.R., Strong C.D, & Dorn G.W. (2023). A human mitofusin 2 mutation can cause mitophagic cardiomyopathy. eLife, 12, e84235.
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