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Mtt assay

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The MTT assay is a colorimetric method used to measure the metabolic activity of cells. It is based on the conversion of a tetrazolium salt (MTT) into a colored formazan product by the mitochondrial enzymes of living cells. The resulting color change can be measured using a spectrophotometer, providing an indication of cell viability and proliferation.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (10 mentions) Microplate reader, by Bio-Rad (5 mentions) Microplate reader, by Agilent Technologies (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Ldh detection kit, by Beyotime (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Multiskan fc, by Thermo Fisher Scientific (2 mentions) Ldh activity kit, by Beyotime (1 mentions) Multiskan spectrum microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions) Mtt solution, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Cisplatin, by Merck Group (1 mentions) Cisplatin, by Beyotime (1 mentions) Infinite m200, by Tecan (1 mentions) Microplate reader, by BMG Labtech (1 mentions) Collagenase, by Merck Group (1 mentions) Ldh assay, by Beyotime (1 mentions) Anti tgf beta, by Abcam (1 mentions) Dulbecco s modified eagle medium, by Merck Group (1 mentions) Mitosox red mitochondrial superoxide indicator, by MedChemExpress (1 mentions)

85 protocols using mtt assay

1

Cell Viability and Cytotoxicity Assays

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Cell viability was analyzed using MTT assays (Beyotime) at 0, 24, 48 and 72 h after transfection. 20 μL of MTT solution was added to each well and cells were incubated for 4 h at 37°C. Supernatants were removed and formazan crystals were dissolved in 150 μL dimethylsulfoxide (DMSO). Absorbance was measured using a Multiskan Spectrum Microplate Spectrophotometer (Thermo Scientific™, USA) at a wavelength of 492 nm.
Cytotoxicity (LDH activity) levels were measured using LDH activity kits (Beyotime). Absorbance was measured using a Multiskan Spectrum Microplate Spectrophotometer (Thermo Scientific™, USA) at a wavelength of 450 nm.
Zheng Z., Qiu K, & Huang W. (2021). Long Non-Coding RNA (lncRNA) RAMS11 Promotes Metastatis and Cell Growth of Prostate Cancer by CBX4 Complex Binding to Top2α. Cancer Management and Research, 13, 913-923.
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2

Cytotoxicity Assay of Cisplatin and LAC in HeLa Cells

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Cell viability was determined by MTT assay. HeLa cells, during the exponential growth phase, were seeded into 96-well culture plates in 100 μl IMDM at a density of 1×104 cells/well. After 24-h incubation, the indicated dose of cisplatin (5 μg/ml) and/or LAC (10 μM) was added for 12-h incubation in four parallel wells. MTT assays (Beyotime Institute of Biotechnology, Haimen, China) were performed as follows: 20 μl MTT solution (5 mg/ml in PBS) was added to the cells for 4 h, after which, 150 μl dimethyl sulfoxide (Beijing Chemical Industry Co., Ltd., Beijing, China) was added to each well. The cells were agitated for 10 min, prior to absorbance measurements at 570 nm using a Microplate Reader (Bio-Rad Laboratories, Hercules, CA, USA). The growth inhibition rate was calculated as % inhibition = 1 − absorbance of experimental group/absorbance of control group × 100. The mean value of the four replica wells was calculated for each treatment group.
XU Y., LI D., ZENG L., WANG C., ZHANG L., WANG Y., YU Y., LIU S, & LI Z. (2014). Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress-associated apoptosis in HeLa cells. Molecular Medicine Reports, 11(1), 189-195.
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3

MTT Assay for Cell Viability

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Transfected Hela and C33A cells were planted at a density of 4,000 cells per well in 96-well plates and assayed using a MTT assays (Beyotime, China) at indicated time (0, 24, 48, and 72 h) following the manufacturer’s description. A microplate reader (Bio-rad) was used to measure the viability of cells through absorbance at 450 nm.
Li M., Wang J., Ma H., Gao L., Zhao K, & Huang T. (2021). Extracellular Vesicles Long Non-Coding RNA AGAP2-AS1 Contributes to Cervical Cancer Cell Proliferation Through Regulating the miR-3064-5p/SIRT1 Axis. Frontiers in Oncology, 11, 684477.
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4

MTT Assay for Cell Viability

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Cell viability was determined by MTT assay. SKOV3, Bel-7402 and HeLa cells in the exponential growth phase were seeded into 96-well culture plates in 100 µl RPMI-1640 at a density of 8×103 cells/well. After 24-h incubation, the indicated dose of BMH-21 was added to the 24-h incubation in four parallel wells. MTT assays (Beyotime Institute of Biotechnology, Haimen, China) were performed as follows: 20 µl MTT solution (5 mg/ml) in phosphate-buffered saline (PBS; Beijing Zhongshan Golden Bridge Biological Technology Co., Ltd., Beijing, China) was added to cells for 4 h, after which 150 µl dimethyl sulfoxide (Beijing Chemical Industry Co., Ltd., Beijing, China) was added to each well. Cells were agitated for 10 min prior to absorbance measurements at 570 nm using a Microplate Reader (Bio-Rad Laboratories, Hercules, CA, USA). The growth inhibition rate was calculated as % inhibition = 1 - absorbance of experimental group / absorbance of control group × 100. The mean value of the four replicate wells was calculated for each treatment group.
Fu X., Xu L., Qi L., Tian H., Yi D., Yu Y., Liu S., Li S., Xu Y, & Wang C. (2017). BMH-21 inhibits viability and induces apoptosis by p53-dependent nucleolar stress responses in SKOV3 ovarian cancer cells. Oncology Reports, 38(2), 859-865.
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5

Cell Viability and Colony Formation

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Cell viability was measured using MTT assays (Beyotime, Shanghai, China). Briefly, Cells were placed in a 96-well plate at a density of 2,000 cell/well to allow cell adherence. After incubating with 15 μl MTT solution (5 mg/ml) (Sigma-Aldrich, MO, USA) in each well for 4 h, the absorbance was measured under the wavelength of 570 nm using a microplate reader. Cells were plated in a six-well plate at a density of 300 cells per well and culture in the colony forming medium (Sigma-Aldrich, USA). After culture, cells were fixed using 4% paraformaldehyde for 15 min and washed with PBS. The colonies were stained with 0.1% crystal violet staining solution for 30 min and finally calculated using ImageJ software.
Wang X., Zhu X, & Zhao Y. (2021). Targeting miR-185-3p Inhibits Head and Neck Squamous Cell Carcinoma by Modulating RAB25. Frontiers in Oncology, 11, 721416.
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6

MiR-141 Regulates Cell Proliferation

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Cells were plated into a 96-well plate at a density of 1,000 cells per well. After 24 h, the cells were transfected with the miR-141 mimic, miR-141 inhibitor or control. Cell proliferation was analyzed after 0, 12, 24 and 48 h using MTT assays (Beyotime Biotechnology, Tianjin, China) according to the manufacturer’s protocol, measuring absorbance at 490 nm. All assays were performed in triplicate wells, and experiments were independently performed at least three times.
Zhao Z., Gao D., Ma T, & Zhang L. (2019). MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma. Aging (Albany NY), 11(3), 921-932.
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7

Evaluating Cisplatin Resistance in Ovarian Cancer

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Cell culture. The cisplatin-sensitive human ovarian cancer cell line SKOV3 and the cisplatin-resistant clone SKOV3/ DDP were obtained from the Chinese Academy of Medical Sciences and the Peking Union Medical College, respectively. Cells were cultured at 37˚C with 5% CO 2 in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FbS; Invitrogen, Carlsbad, CA, USA). SKOV3/DDP cells were maintained in the same medium containing 1 µg/ml cisplatin (Sigma, St. Louis, MO, USA) to maintain the multidrug-resistant phenotype.
Cell viability assays. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Exponentially growing cells were seeded into 96-well culture plates in 100 µl medium at a density of 1x10 4 cells/well. After 16-24 h, varying concentrations of cisplatin were added to quadruplicate wells and cells were incubated for 24 or 48 h. For the MTT assays (beyotime, Shanghai, China), 20 µl/well MTT [5 mg/ml in phosphate-buffered saline (PbS)] was added to the cells and incubated for 4 h. Dimethylsulfoxide (150 µl/well; beijing Chemical Industry, beijing, China) was then added, the plates were shaken at room temperature for 10 min, and the absorbance was measured at a 570-nm wavelength using a microplate reader ( bioTek Instruments, Winooski, VT, USA).
Xu Y., Wang C., Su J., Xie Q., Ma L., Zeng L., Yu Y., Liu S., Li S., Li Z, & Sun L. (2015). Tolerance to endoplasmic reticulum stress mediates cisplatin resistance in human ovarian cancer cells by maintaining endoplasmic reticulum and mitochondrial homeostasis. Oncology reports, 34(6).
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8

Sulforaphane Cytotoxicity Evaluation

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The cells (5 × 103 cells per well) were seeded in 96-well plate for 24 h, then treated with different concentrations of SFN for 0, 24, 48, 72 and 96 h. Thereafter, cell proliferation was measured using the MTT assay according to the kit instructions (Beyotime Biotechnology, Shanghai, China).
Wang Y., Wu H., Dong N., Su X., Duan M., Wei Y., Wei J., Liu G., Peng Q, & Zhao Y. (2021). Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells. Scientific Reports, 11, 2504.
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9

Cell Viability Assay of Anagliptin

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MTT assay (Beyotime Institute of Biotechnology) was utilized to detect cell viability. Briefly, HPMVECs were seeded into 96-well plates (5x104 cells/well) and incubated at 37˚C to 90% confluence. Subsequently, cells were exposed to various concentrations of anagliptin (1, 10, 50 or 100 µM) or LPS ± anagliptin for 24 h. Then, 50 µl MTT solution was added to each well and maintained for 3 h at 37˚C. Cells were exposed to 150 µl DMSO and shaken on an orbital shaker for 15 min, then absorbance of each well was measured at 590 nm.
Zhang J, & Liu L. (2021). Anagliptin alleviates lipopolysaccharide-induced inflammation, apoptosis and endothelial dysfunction of lung microvascular endothelial cells. Experimental and Therapeutic Medicine, 22(6), 1472.
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10

Measuring PC12 Cell Viability by MTT Assay

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The cell viability of PC12 cells under different conditions was assessed by MTT assay (Beyotime Institute of Biotechnology; cat. no. C0009), according to the manufacturer's protocol. In brief, cells that transfected with or without indicated plasmids were seeded in 96-well plates for 24 h and then incubated with 20 µl MTT solution for 4 h. After discarding MTT medium dissolving the remaining formazan crystals with DMSO, the cell viability was tested at 570 nm by a microplate reader (Thermo Fisher Scientific, Inc).
Li Y., Sun J., Gu L, & Gao X. (2020). Protective effect of CTRP6 on cerebral ischemia/reperfusion injury by attenuating inflammation, oxidative stress and apoptosis in PC12 cells. Molecular Medicine Reports, 22(1), 344-352.
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