The cryo-EM sample preparation followed our previous publication (Benecke et al., 2022 (link)). A total of 4 μL aliquot of each sample was adsorbed onto holey carbon-coated grid (Ted Pella Inc., Redding, United States) blotted with Whatman 1 filter paper and vitrified into liquid ethane at −178°C using a Leica GP plunger (Leica Microsystems GmbH, Wetzlar, Germany). Frozen grids were transferred onto a Talos electron microscope (FEI/Thermo Fisher Scientific Inc., Waltham, United States) using a Gatan 626 cryo-holder (Gatan, Inc., Pleasanton, United States). Electron micrographs were recorded at an accelerating voltage of 200 kV, using a low-dose system (20 e−/Å2) and keeping the sample at low temperature. Micrographs were recorded on a CETA camera (Thermo Fisher Scientific Inc., Waltham, United States).
Holey carbon coated grid
Manufactured by Ted Pella
Sourced in United States
The Holey carbon-coated grid is a laboratory equipment product designed for use in various microscopy techniques. It features a carbon-coated grid with pre-defined holes or perforations that allow for the placement and analysis of samples.
Automatically generated - may contain errors
3 protocols using holey carbon coated grid
1
Cryo-EM Sample Preparation Protocol
2
Cryo-EM Imaging of Virus Particles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey, Tedpella, USA), blotted with Whatman 1 filter paper and vitrified into liquid ethane at −180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI, USA) using a Gatan 626 cryo-holder (GATAN, USA). Electron micrographs were recorded at an accelerating voltage of 200 kV using a low-dose system (40 e-/Å2) and keeping the sample at −175 °C. Defocus values were −2 to 3 µm. Micrographs were recorded on 4K x 4K Ceta CMOS camera.
3
Cryo-EM Sample Vitrification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 4 µl aliquot of sample was adsorbed onto holey carbon-coated grid (Ted Pella, Inc.) blotted with Whatman 1 filter paper and vitrified into liquid ethane at -178°C using a Leica GP plunger (Leica Microsystems GmbH). Frozen grids were transferred onto a Talos electron microscope (FEI) using a Gatan 626 cryo-holder. Electron micrographs were recorded at an accelerating voltage of 200 kV, using a low-dose system (20 e-/Å2) and keeping the sample at low temperature. Micrographs were recorded on a CETA camera (Thermo Fisher Scientific, Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!