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68 protocols using sodium potassium tartrate

1

Quantification of Amino Acid Derivatives

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Alpha-alanyL-histidine (Ala-His, G-1245) was purchased from Bachem Americas, Inc., (Torrance, CA). 4-Fluoro-7-sulfamoylbenzofurazan (ABD-F) was purchased from Wako Chemicals USA, Inc., (Richmond, VA, USA). L-carnosine (>99%), L-alanine (>98%), L-histidine (>99%), L-glutathione reduced (GSH), glucose, fructose, sucrose, o-phthalaldehyde (OPA, solution complete), 3,5-dinitrosalicylic acid (DNS), diethylenetriaminepentaacetic acid (DTPA), sodium hydroxide, sodium potassium tartrate, sodium potassium tartrate, sodium phosphate dibasic (Na2HPO4), sodium chloride (NaCl), potassium chloride (KCl), potassium phosphate monobasic (KH2PO4), and sodium azide were obtained from Sigma-Aldrich Co., (St. Louis, MO, USA). All solutions were prepared using ultrapure distilled deionized water (18.2 MΩ cm).
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2

Phytochemical Analysis and Cytotoxicity

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All organic solvents, HCL and H2SO4 used for the extraction were obtained from RCI Labscan (Samut Sakhon, Thailand). Sodium Hydroxide and FeCl3 were purchased from Q RëC™ (New Zealand). KOH was purchased from KemAus™ (Cherrybrook, Australia). Dragendorff’s reagent and sodium-potassium tartrate were purchased from Merck (Darmstadt, Germany). DMSO was purchased from Lab-Scan. Dulbecco’s modified Eagle’s medium (DMEM), RPMI Media 1640, 0.25% trypsin-EDTA (1×), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from GIBCO® (Invitrogen, Grand Island, NY, USA). FITC-conjugated Annexin V and propidium iodide (PI) were purchased from BioLegend (San Diego, CA, USA). Neutral red (NR) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). TLC silica gel 60 F254 and silica gel 60 (grain fraction 0.2–0.5 mm) were purchased from Merck (Darmstadt, Germany).
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3

Inulinase Production from Aspergillus niger

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Inulinase from Aspergillus niger (EC 3.2.1.7 and EC 3.2.1.80) was obtained from Sigma-Aldrich. Glutaraldehyde (25% v/v in water) while sodium-potassium tartrate and 3,5-dinitrosalicylic acid (DNS) were purchased from Merck. Inulin (from Chicory roots) was received from Fluka Company (Fluka, Switzerland). Wheat gluten hydrolysates were produced via enzymatic hydrolysis of wheat gluten by Endopeptidases (Alcalase 2.4 L and Neutrase 0.8 L, provided from Novozymes Company) by Food Science and Technology Lab of Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST, Tehran, Iran). All other reagents and solvents used were of analytical grade and obtained from Merck and Sigma-Aldrich.
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4

Extraction and Characterization of Dragon Fruit Bioactive Compounds

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All chemicals and reagent were in analytical grade. Bradford reagent, bovine serum albumin (BSA), and 3,5-dinitrosalicylic acid (DNS) were obtained from Sigma Chemical Co., (St. Louis, MO, USA). Dibasic sodium phosphate (Na2HPO4·2H20), monobasic sodium phosphate (NaH2PO4·H20), sodium acetate, acetic acid, sodium citrate, citric acid, soluble starch, maltose, and sodium potassium tartrate (NaKC4H4O6·4H2O) were obtained from Merck (Darmstadt, Germany). Red dragon fruits (Hylocereus polyrhizus) were purchased from Passer Brong (Selangor, Malaysia). Ripened dragon fruits were selected based on the size uniformity at the same stage of ripening and free of visual defects. The fruits were stored in a cold room at 4°C until used for extraction procedure.
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5

Pectin Extraction from Guava Fruit

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All chemicals and reagents were of analytical grade. Bradford reagent, 3,5-dinitrosalicylic acid (DNS), bovine serum albumin (BSA), and polygalacturonic acid were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Triton X-100, Tween-80, sodium dodecyl sulphate (SDS), acetic acid, sodium citrate, citric acid, D-galacturonic acid, and sodium potassium tartrate (NaKC4H4O6·4H2O) were obtained from Merck (Darmstadt, Germany). Guava (Psidium guajava) fruits were purchased from local market (Selangor, Malaysia). Ripened guava fruits free of visual defects were selected based on the size uniformity at the same stage of ripening. The fruits were stored in a cold room at 4°C until use for the extraction procedure.
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6

Analysis of Essential Oil Compounds

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Aromadendrene, Borneol, α-Bisabolol, Camphene, Carvacrol, Caryophyllene oxide, 1,8-Cineole, α-Humulene, Limonene, Linalool, (E)-β-Ocimene, α-Phellandrene, α-Pinene, β-Pinene, Sabinene, α-Terpinene, γ- Terpinene, Terpinen-4-ol, α-Terpineol, α-Thujene, Thymol, soluble starch, DNSA (dinitrosalicylic acid), were purchased from Sigma Aldrich (Milan, Italy). Sodium tartrate, sodium potassium tartrate, sodium acetate were bought from Merck (Darmstadt, Germany).
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7

Extraction and Fractionation of Plant Compounds

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The solvents used for extraction and fractionation of plant material were of high-performance liquid chromatography (HPLC) grade. XO, xanthine, allopurinol, acarbose, and starch were purchased from Sigma-Aldrich, alpha-amylase from MP Biomedicals, sodium potassium tartrate from Merck and 3,5-dinitrosalisylic acid (DNS) from Fisher Scientific. All other reagents were of analytical grades.
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8

HPLC and Enzymatic Bioassays of Phenolics

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HPLC standards, apigenin, caffeic acid, chlorogenic acid, trans-cinnamic acid, ferulic acid, gallic acid, kaempferol, luteolin, naringenin, p-coumaric acid, vanillic acid, and 4-hydroxybenzoic acid were purchased from Sigma Aldrich (Santiago, Chile). Aluminum chloride hexahydrate, anhydrous sodium carbonate, ethanol absolute, formic acid, ferric chloride hexahydrate, Folin–Ciocalteu reagent, hydrochloric acid, HPLC solvents, sodium hydroxide, sodium nitrate, and sodium-potassium tartrate were obtained from Merck (Santiago, Chile). Alpha-amylase from porcine pancreas, 3,5-dinitrosalicylic acid, 4-nitrophenyl-α-D-glucopyranoside, alpha-glucosidase from Saccharomyces cerevisiae, ABTS (2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonate)), acarbose, glacial acetic acid, potassium persulfate, starch, TPTZ (1,3,5-triphenyltetrazolium chloride), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were supplied by Sigma-Aldrich (Santiago, Chile).
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9

Analytical Evaluation of Sweeteners

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All chemical reagents used in this study were of analytical grade. Ethanol 96 %, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), K2S2O8, Folin–Ciocalteu reagent, ascorbic acid, 3,5-dinitrosalicylic acid (DNS), sodium potassium tartrate, NaOH, Na2CO3, KCl, CH3COONa, KMnO4, 36.5 % HCl, glacial acetic acid, H2SO4 (0,1N) were purchased from Merck (Darmstadt, Germany). Ultrapure water was used in all the experiments. Sucrose was purchased from a local market in Cluj-Napoca, while all the other sweeteners used in this study were purchased from a health food store.
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10

Colorimetric Assay for Biochemical Analysis

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The AR grade chemicals and supplier details are as follows: Oxybenzene (Nice Chemicals, Mumbai, India), Anhydrous sodium carbonate (Rankem, Mumbai, India), Potassium hydrogen phosphate (s.d.Fine Chem Ltd, Bengaluru, India), Sodium Bicarbonate (Rankem, Mumbai, India), Copper sulphate, sodium potassium tartrate (Merck, Pune, India), 4-aminoantipyrine, Boric acid, Potassium ferricyanide, Potassium dihydrogen phosphate (Hi media laboratories, Bengaluru, India), di-sodium hydroxide (Merck, Pune, India), Sodium Bicarbonate, and Folin reagent (Merck, Pune, India).
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