Allegra x 22r

Manufactured by Beckman Coulter

Sourced in United States, Germany

The Allegra X-22R is a high-performance refrigerated benchtop centrifuge designed for a wide range of general laboratory applications. It features a maximum speed of 22,000 rpm and a maximum relative centrifugal force (RCF) of 53,800 x g. The Allegra X-22R is capable of accommodating a variety of rotor options to meet diverse sample processing needs.

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Lab products found in correlation

Rnase a, by Merck Group (4 mentions) Pi staining solution, by Merck Group (4 mentions) Rotor sx4250, by Beckman Coulter (2 mentions) Microfuge 22r, by Beckman Coulter (2 mentions) Facsaria, by BD (2 mentions) Du730, by Beckman Coulter (1 mentions) S 4800, by Hitachi (1 mentions) D8 advance, by Bruker (1 mentions) Qiaamp minelute virus spin kit, by Qiagen (1 mentions) 0.45 μm pvdf membrane, by Merck Group (1 mentions) Inb 400, by Memmert (1 mentions) 2414 refractive index detector, by Waters Corporation (1 mentions) 717 autosampler, by Waters Corporation (1 mentions) Acquity uplc beh c18 column, by Waters Corporation (1 mentions) Beh c18 vanguard guard column, by Waters Corporation (1 mentions) Waters 515 hplc pump, by Waters Corporation (1 mentions) Bio beads s x3, by Bio-Rad (1 mentions) Abi 4000 q trap, by Thermo Fisher Scientific (1 mentions) Porcine insulin elisa kit, by Mercodia (1 mentions) Versamax elisa microplate reader, by Molecular Devices (1 mentions)

Spelling variants of this product

Allegra X-22R centrifuge (28 citations) Allegra 22R (4 citations) Allegra™ X-22R Benchtop Centrifuges (2 citations) Allegra X-22R with SX4250 rotor (1 citations)

51 protocols using allegra x 22r

Assessing Emulsifying Properties of LPI

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Emulsifying properties were assessed using the protocol described by Zhao et al. [20 (link)], with modifications. In total, 15 mL of LPI solution (1%) at pH 7 was placed in a 50 mL volume falcon tube, and homogenized for 15 s (UltraTurrax, 12,000 rpm). Then, 15 mL of soybean oil were added slowly, and the mixture was homogenized again for 1 min. The emulsion was centrifuged at 1300× g for 5 min (Beckman Coulter, model Allegra X-22R, rotor SX4250) and at room temperature. The emulsifying capacity was calculated according to the next equation.

E m u l s i f y i n g c a p a c i t y (%) = \frac{V o l u m e o f t h e e m u l s i f i e d l a y e r a f t e r c e n t r i f u g a t i o n}{V o l u m e o f e m u l s i o n b e f o r e c e n t r i f u g a t i o n} \times 100

For the determination of emulsifying stability, the emulsion obtained after homogenization was heated for 30 min at 80 °C. Then, they were cooled at room temperature and centrifuged (1300× g for 5 min; Beckman Coulter, model Allegra X-22R, rotor SX4250), and the emulsifying stability was calculated according to the next equation.

E m u l s i f y i n g s t a b i l i t y (%) = \frac{V o l u m e o f t h e e m u l s i f i e d l a y e r a f t e r h e a t i n g}{V o l u m e o f e m u l s i o n b e f o r e c e n t r i f u g a t i o n} \times 100

Domínguez R., Bermúdez R., Pateiro M., Lucas-González R, & Lorenzo J.M. (2023). Optimization and Characterization of Lupin Protein Isolate Obtained Using Alkaline Solubilization-Isoelectric Precipitation. Foods, 12(20), 3875.

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Synthesis and Characterization of Zinc-Based Photocatalyst

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In this study, zinc acetate dihydrate was purchased from Wuxi ZhanWang Chemical Reagent Co., LTD. 2-methylimidazole was provided by Shanghai Bide Pharmaceutical Technology Co., LTD. All other reagents used were purchased from Sinopharm Group Chemical Reagent Co., LTD.
A Newport 94023A (USA) device was used as the sunlight stimulator. The UV–vis spectroscopy and centrifugal operation were carried out on DU730 and AllegraTM X-22R devices (BeckMan Coulter). XRD, XPS and SEM were performed on D8 Advance (Bruker, Germany), Thermo Escalab 250 XI (PHI-5000 Versaprobe, UK) and S-4800 (Hitachi, Japan) devices, respectively. An in-house FORTRAN code was developed to implement the field-only surface integral method used to calculate the electric field on the particle surface.

Hu B., Sun Q., Zuo C., Pei Y., Yang S., Zheng H, & Liu F. (2019). A highly efficient porous rod-like Ce-doped ZnO photocatalyst for the degradation of dye contaminants in water. Beilstein Journal of Nanotechnology, 10, 1157-1165.

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Virus-like Particle Enrichment and Sequencing from Fecal Samples

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Virus-like particles of inactivated fecal samples were enriched and purified according to the modified workflow we developed in a previous study.^{25 (link)} In brief, each fecal sample (approximately 500 mg) was resuspended in 15 ml PBS and centrifuged at 4,500 rpm for 10 min at the 4°C to remove large food residues (Beckman Coulter Allegra^TM X-22 R). The supernatant was transferred to fresh tubes and centrifuged at 4,500 rpm for 10 min at the 4°C again. The supernatant was then filtered through a 0.45 μm PVDF membrane (Millipore) twice and centrifuged at 180,000 × g at 4°C for 3 h (Beckman Coulter XP-100). The pellets were resuspended in 400 µl PBS and treated with RNase A and DNase I at 37°C for 30 min. Viral nucleic acids, including DNA and RNA, were extracted by using a QIAamp MinElute Virus Spin Kit (Qiagen). The viral nucleic acids underwent reverse transcription, random amplification and agarose gel electrophoresis to obtain cDNA to prepare the sequencing library.
PromethION library preparation was performed according to the manufacturer’s instructions for barcoding cDNA/DNA and native DNA (SQK-LSK109, EXP-NBD104 and EXP-NBD114). When multiplexing, all the samples were pooled together. Oxford Nanopore Technologies (ONT) MinKNOW software (v.19.10.1) was used to collect raw sequencing data, and Guppy (v.3.2.4) was used for real-time base calling of the raw data.

Cao J., Wang C., Zhang Y., Lei G., Xu K., Zhao N., Lu J., Meng F., Yu L., Yan J., Bai C., Zhang S., Zhang N., Gong Y., Bi Y., Shi Y., Chen Z., Dai L., Wang J, & Yang P. (2021). Integrated gut virome and bacteriome dynamics in COVID-19 patients. Gut Microbes, 13(1), 1887722.

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Evaluating Graft Material Ionic Release

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A sample of 0.5 g from each graft material was added to 10 mL of PBS and maintained in a drying oven (INB 400, Memmert, Germany) at 37°C. The samples were collected on days 3, 7, and 14. On day 3, PBS containing 0.5 g graft materials was centrifuged (AllegraTM, X-22R, Beckman Coulter, CA, USA) at 1,000 rpm for 5 min and 10 mL of supernatant was obtained. The unchanged lot of 10 mL PBS was added to the same 0.5 g of graft materials, then stored for another four days. After 7 days, PBS containing 0.5 g graft materials was centrifuged at 1,000 rpm for 5 min and 10 mL of supernatant was obtained. The same procedure was repeated, and the sample was stored for another 7 days.
At each time interval, the same lot PBS was used as the blanks for measuring amounts of calcium (Ca) and phosphorus (P) ions presence and compared with the amounts of Ca and P ions from PBS containing 0.5 g graft materials. The representative amounts of Ca and P ions released in the supernatant were once measured at days 3, 7, and 14 by inductively coupled plasma-mass spectroscopy (ICP-MS) (7500ce models, Agilent) and ion chromatography (IC) (ICS-3000 models, DIONEX), respectively.

Khanijou M., Zhang R., Boonsiriseth K., Srisatjaluk R.L., Suphangul S., Pairuchvej V., Wongsirichat N, & Seriwatanachai D. (2021). Physicochemical and osteogenic properties of chairside processed tooth derived bone substitute and bone graft materials. Dental materials journal, 40(1).

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Bound Phenolics Extraction from Triticale

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Bound phenolics in the raw and cooked triticale samples were extracted according to Zieliński et al. [20 (link)] with a slight alteration. The mixture of the precipitate from the extraction of free phenolics and 20 mL of 4 M NaOH was sonicated for 90 min at 40 °C, and then the pH of the solution was adjusted to 2.0 using concentrated HCl, followed by centrifugation (Allegra X-22R, Beckman Coulter, Brea, CA, USA) at 3000× g for 20 min at 4 °C. Subsequently, the released phenolics were extracted three times with 30 mL of ethyl acetate from the supernatant. The combined ethyl acetate extracts were dried using a rotary evaporator (RE-52AA, Yarong, Shanghai, China), and then the dry residue was dissolved in 1 mL of methanol/water (1:9, v/v) containing the IS (paracetamol, 10 μg/mL) and passed through a 0.45 µm membrane (Jinteng, Tianjing, China).

Li H., Mao Y., Ma D., Li H., Liu R, & Siriamornpun S. (2024). Impact of Cooking Methods on Phenolic Acid Composition, Antioxidant Activity, and Starch Digestibility of Chinese Triticale Porridges: A Comparative Study between Atmospheric Pressure and High Pressure Boiling. Foods, 13(2), 230.

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Triticale Phenolic Compounds Extraction

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Free phenolics in the raw and cooked triticale samples were extracted according to Tian et al. [19 (link)] with a little modification. In total, 1 g of triticale powder was mixed with 20 mL of 70% methanol and sonicated for 15 min at 40 °C with an ultrasonic extractor (XH-2008D, Xianghu Technologies, Beijing, China), followed by centrifugation (Allegra X-22R, Beckman Coulter, Brea, CA, USA) at 3000× g for 10 min at 4 °C. The residue was extracted again following the same method, and the combined supernatants were evaporated to less than 2 mL under nitrogen at 30 °C. Afterwards, the concentrate was mixed well with 2 mL of 1% acetic acid and injected into an SPE cartridge (Oasis HLB, 6 cc, 200 mg; Waters, Milford, MA, USA). To remove sugars and other polar constituents, the cartridge was washed with 3 mL of water. Subsequently, 2 mL of methanol/1% acetic acid (9:1, v/v) was used to elute the absorbed compounds. Finally, after the eluates were evaporated to dryness under nitrogen, the residues were redissolved in 200 μL of methanol/water (1:9, v/v) containing the IS (paracetamol, 10 μg/mL) and then passed through a 0.45 µm membrane (Jinteng, Tianjing, China).

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Analysis of Phthalate Residues using LC-MS/MS

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Samples were prepared by using a centrifuge (Allegra X-22R, Beckman Coulter Inc., Fullerton, CA, USA), a nitrogen evaporator (N-Evap-111, Organomation Associates Inc., Berlin, MA, USA), a vortex mixer (type 37600 mixer, Barnstead Thermolyne LLC, Dubuque, Iowa, USA), and a nitrogen generator (Model 05B, System Instruments Co., Tokyo, Japan). LC-MS/MS consisted of a mass spectrometer (ABI 4000 QTRAP, Applied Biosystems, Foster City, CA, USA) in electrospray ionization (ESI) mode and an Acquity Ultra Performance LC system (Agilent Technologies 1200, Agilent Technologies, Palo Alto, CA, USA). To measure the residual amounts of phthalates in the samples, chromatographic separation was conducted in an analytical Acquity UPLC BEH C18 column (2.1 mm × 100 mm × 1.7 µm, Waters Corp., Milford, MA, USA) equipped with a BEH C18 vanguard guard column (2.1 mm × 5 mm × 1.7 µm,, Waters Corp., Milford, MA, USA). Gel permeation chromatography (GPC) (Waters 515 HPLC pump, 717 autosampler, 2414 refractive index detector, Waters Corp., Milford, MA, USA) was treated further to eliminate potential chromatographic interference of lipids for extraction and cleanup with a clean-up column (400 mm × 25 mm, 200–400 mesh, Bio-Beads S-X3, Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Tsai M.Y., Ho C.H., Chang H.Y., Yang W.C., Lin C.F., Lin C.T., Xue Y.J., Lai J.M., Wang J.H, & Chang G.R. (2019). Analysis of Pollution of Phthalates in Pork and Chicken in Taiwan Using Liquid Chromatography–Tandem Mass Spectrometry and Assessment of Health Risk. Molecules, 24(21), 3817.

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Plasma Insulin and Glucagon Quantification

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Whole blood was collected in 4 ml K2 EDTA tubes, gently rocked and immediately centrifuged at 4°C for 15 min at 1,600 RPM (Beckman Coulter, Allegra X-22R, Indianapolis, IN). Plasma was collected, immediately snap frozen in liquid N₂ and stored at −80° C. A two-site ELISA was performed using a porcine insulin ELISA kit (Mercodia, Winston Salem, NC # 10-1200-01). This porcine insulin ELISA has virtually no cross-reactivity with porcine pro-insulin or C-peptide. A colorimetric glucagon ELISA was performed using a glucagon ELISA kit (Phoenix Pharmaceuticals, Burlingame, CA # EK-028-02). All ELISA procedures were performed according to manufacturer recommendations. Plates were read on VersaMax ELISA Microplate Reader (Molecular Devices, v5, Sunnyvale, CA).

Uc A., Olivier A.K., Griffin M.A., Meyerholz D.K., Yao J., Abu-El-Haija M., Buchanan K.M., Vanegas Calderón O.G., Abu-El-Haija M., Pezzulo A.A., Reznikov L.R., Hoegger M.J., Rector M.V., Ostedgaard L.S., Taft P.J., Gansemer N.D., Ludwig P.S., Hornick E.E., Stoltz D.A., Ode K.L., Welsh M.J., Engelhardt J.F, & Norris A.W. (2015). GLYCEMIC REGULATION AND INSULIN SECRETION ARE ABNORMAL IN CYSTIC FIBROSIS PIGS DESPITE SPARING OF ISLET CELL MASS. Clinical science (London, England : 1979), 128(2), 131-142.

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Rat Blood Collection for Vaccine Study

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A 25-gauge butterfly needle (Surshield Safety Winged Infusion Set, Terumo, Somerset, NJ) was used to collect 1 to 1.5 ml of blood via a lateral tail vein. Rats were bled on days 0 and 14 before first and second doses of vaccination, respectively. Blood (5 to 10 ml) was collected at the terminal bleed on day 29 via the vena cava. Blood was centrifuged at 10,000 rpm for 10 min (Allegra X-22R, Beckman Coulter, Brea, CA) to separate the serum. Serum was collected, aliquoted, and stored at −80°C. Freeze-thaw cycles were kept minimal to ensure the integrity of the serum.

Lallow E.O., Jhumur N.C., Ahmed I., Kudchodkar S.B., Roberts C.C., Jeong M., Melnik J.M., Park S.H., Muthumani K., Shan J.W., Zahn J.D., Shreiber D.I., Singer J.P., Park Y.K., Maslow J.N, & Lin H. (2021). Novel suction-based in vivo cutaneous DNA transfection platform. Science Advances, 7(45), eabj0611.

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Cell Cycle Analysis by Flow Cytometry

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Each group of cells was seeded at 3×10⁵ cells/well in six-well plates and cultured until 85% confluence was reached. The cells were washed three times with PBS, prior to collection by centrifugation (Allegra X-22R, Beckman Coulter, Miami, FL, USA) at 1,000 × g for 5 min. The cell pellets were subsequently resuspended in 1 ml PBS, fixed in 70% ice-cold ethanol and kept in a freezer for >48 h. Prior to flow cytometric analysis, the fixed cells were centrifuged at 1,000 × g for 5 min, washed twice with PBS, and resuspended in PI staining solution (Sigma-Aldrich), containing 50 µl/ml PI and 250 µg/ml RNase A (Sigma-Aldrich). The cell suspension, which was protected from the light, was incubated for 30 min at 4°C and analysed by fluorescence-activated cell sorting (FACS) using a BD FACSAria cell sorter (BD Biosciences, Frankin Lakes, NJ, USA). A total of 20,000 events were acquired for analysis using CellQuest software version 2.0 (BD Biosciences).

HOU L., LIU T, & WANG J. (2015). Isoflurane suppresses the self-renewal of normal mouse neural stem cells in a p53-dependent manner by activating the Lkb1-p53-p21 signalling pathway. Molecular Medicine Reports, 12(5), 7412-7418.

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