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2 protocols using dm3614p

1

Multiparametric Immunofluorescence Staining

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Primary antibodies: rat anti-CD31 (DM3614P, Dianova, Hamburg, Germany), goat anti-CD32b (AF1460, R&D Systems, Minneapolis, MN, USA), goat anti-Lama4 (AF3837, R&D Systems, Minneapolis, MN, USA), rabbit anti-Desmin (ab32362, Abcam, Cambridge, UK), rabbit anti-CD3 (100202, Biolegend, San Diego, CA, USA), rat anti-CD4 (100402, Biolegend, San Diego, CA, USA), rat anti-CD8a (100802, Biolegend, San Diego, CA, USA), rabbit anti-F4/80 (30325S, Cell Signaling, Danvers, MA, USA), rat anti-CD11b (101202, Biolegend, San Diego, CA, USA), rabbit anti-CD11c (97585S, Cell Signaling, Danvers, MA, USA), rat anti-MHCII (14-5321-85, eBioscience, Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-FoxP3 (12653, Cell Signaling, Danvers, MA, USA), rabbit anti-CD45 (ab10558, abcam, Cambridge, UK), rat anti-Ly6C (ab15627, abcam, Cambridge, UK), rat anti-Gr1 (ab 25377, abcam, Cambridge, UK), goat anti-Reelin (AF3820, R&D Systems, Minneapolis, MN, USA), goat anti-Periostin (AF2955, R&D Systems, Minneapolis, MN, USA), rabbit anti-TGFBI (ab170874, abcam, Cambridge, UK). Secondary antibodies: donkey Alexa-Fluor 488, Alexa-Fluor 647, and Cy3-conjugated secondary antibodies were purchased from Dianova (Hamburg, Germany).
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2

Detailed Immunofluorescence Staining Protocol

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Primary antibodies: Rabbit anti-cleaved Caspase 3 (9661S, Cell Signaling Technology, USA), rabbit anti-Ki-67 (ab16667, Abcam, UK), rat anti-CD31 (DM3614P, Dianova, Germany), goat anti-CD32b (AF1460, R&D Systems, USA), rabbit anti-Stabilin-2 peptide 15 antibody [33 (link)], goat anti-Lyve1 (AF2125, R&D Systems, USA), rat anti-Endomucin (14–5851-82, Thermo Fisher Scientific, USA), rabbit anti-TRP-2 (ab74073, Abcam, UK), goat anti-Lama4 (AF3837, R&D Systems, USA), rabbit anti-Desmin (ab15200, Abcam, UK), rabbit anti-Fibronectin (ab23750, Abcam, UK), rabbit anti-Collagen I (R1038, Acris Antibodies, Germany), rabbit anti-Collagen III (R1040, Acris Antibodies, Germany), rabbit anti-Collagen IV (NB120-6586, Novus Biologicals, Germany). Secondary antibodies: Alexa-Fluor 488, Alexa-Fluor 647 and Cy3-conjugated secondary antibodies were purchased from Dianova (Germany).
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