2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid

Ethanol, hydrochloric acid (37%), and acetone of HPLC grade were purchased from Scharlab Chemie (Barcelona, Spain). Gallic acid, Folin-Ciocalteu reagent, epicatechin, vanillin, polyethylene glycol (8000 Da), polyethylene glycol (4000 Da), twin20 (1228 Da), ethylene glycol (62 Da), dextran (50,000 Da), iron(III) chloride, sodium carbonate, hydrogen peroxide, 4-dimethylaminocinnamaldehyde (DMAC), 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox), potassium persulfate, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) and diammonium salt (ABTS)were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Sodium dihydrogen phosphate dihydrate and dipotassium hydrogen phosphate were obtained from Merck (Hesse, Darmstadt, Germany).
MEthanol (99.99%), formic acid, acetonitrile, and butanol of HPLC grade were supplied by Fisher Scientific (Midlands, Leicestershire, UK). Ultrapure water (18.2 MΩ/cm) was obtained from a Millipore system (Millipore, Billerica, MA, USA). Promod 439 L^TM enzyme was a kind donation of Biocatalysts Ltd. (Wales, Cardiff, UK).

2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), sulfuric acid, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide (K₃[Fe(CN)₆]), sodium carbonate, caffeic acid, p-coumaric acid, quercetin, gallic acid, cinnamic acid, naringenin, pinocembrin, galangin, rutin, pinobanksin, and chlorogenic acid were purchased from Sigma-Aldrich, Germany. Trichloroacetic acid, trisodium phosphate (Na₃PO₄), potassium dihydrogen phosphate (KH₂PO₄), and dipotassium hydrogen phosphate anhydrous (K₂HPO₄) were purchased from VWR, Leuven, Belgium, and chrysin was purchased from AbCam, UK. Ferulic acid was purchased from Acros Organics, USA. Iron (III) chloride was purchased from Buchs, Switzerland. Ammonium heptamolybdate ((NH₄)₆Mo₇O₂₄) was purchased from Pronalab, Lisbon, Portugal. Folin-Ciocalteu's phenol and AlCl3 were purchased from Panreac Quımica, Montcada i Reixac, Barcelona, Spain. Ascorbic acid, cell culture medium (DMEM and RPMI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and triphenyltetrazolium chloride (TTC) were purchased from Sigma-Aldrich, USA. TNF-α, IL-6, and IL-10 kits were purchased from R&D Systems, Inc., USA. Mueller Hinton broth and Mueller Hinton agar were purchased from Biokar Diagnostics (Beauvais, France).

Saline peptone water, Brain Heart Infusion (BHI), Tryptic Soy Broth (TSB) supplemented with Yeast, Plate Count Agar (PCA), Tryptone Sulphite Neomycin (TSN), Dextrose Sabouraud (DS), and Mannitol Egg Yolk Polymyxin (MYP) mediums were obtained from Merck KGaA, (Darmstadt, Germany). Acetonitrile; amphotericin B solution (250 μg/mL); 2,2′-Azinobis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS); Fetal Bovine Serum (FBS); glacial acetic acid; hydrochloric acid; 6-hydroxy 2,5,7,8-tetramethyl-2-carboxylic acid (Trolox); L-glutamine solution (200 mM); MEM (Minimum Essential Medium); methanol; penicillin (10,000 U/mL); pure phenolic compounds (caffeic acid, catechin, epicatechin, epicatechin gallate, ethyl gallate, gallic acid, gentisic acid, p-coumaric acid, procyanidin B1, procyanidin B2, protocatechuic acid, syringic acid, t-resveratrol, t-piceid, and vallinic acid); streptomycin solution (P/S; 100 mg/mL); and 2,4,6-Tris (2-pyridyl)-S-triazine (TPTZ) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Folin–Ciocalteu (FC) reagent, 70% (v/v), iron (III)-chloride acid (FeCl3), iron (II)-sulphate (FeSO₄), sodium acetate (NaC₂H₃O₂), and sodium carbonate (Na₂CO₃) were purchased from Panreac Quimica S.L.U. (Barcelona, Spain). Malvidin-3-glucoside was obtained from Estrasynthese (Genay, France).