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2 protocols using alexa conjugated anti mouse and anti rabbit igg

1

Integrin and Apoptosis Marker Detection

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Antibodies against integrins (αv, α5, α6, β1, β3, β4, and β5), the apoptosis-related protein cleaved caspase-8, and the autophagy marker LC3 were all purchased from Cell Signaling Technology (CST, Tokyo, Japan). Alexa-conjugated anti-mouse and anti-rabbit IgG were purchased from Life Technologies (Eugene, OR, USA). Recombinant integrin αvβ3 and B7-H4 were purchased from R&D Systems (Minneapolis, MN, USA). B7-H4 siRNA and non-targeting control siRNA were purchased from Dharmacon (Lafayette, CO, USA).
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2

Autophagy and Apoptosis Pathway Proteins

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The antibodies against autophagy-related proteins (Beclin1, ATG3, ATG 5, ATG 7, ATG 9A, ATG 12, ATG 13, LC3, and HSPB8), apoptosis-related proteins (caspase-9, cleaved caspase-9, caspase-7, cleaved caspase-7, caspase-3, and cleaved caspase-3) and RAB7 were purchased from Cell Signaling Technology (CST, Japan). The anti-actin antibody was purchased from Santa Cruz. The Alexa-conjugated anti-mouse and anti-rabbit IgG were purchased from Life Technologies. Small interfering RNA (siRNA) for ATG9A, HSPB8, LC3, RAB7 and non-targeting control siRNA were purchased from Thermo Scientific (Lafayette, CO). siRNAs for Beclin1 and ATG5 were purchased from CST. Gefitinib was purchased from Cayman chemical (MI).
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