A549 cell viability was measured using Alamar blue reagent (Hi Media Laboratories, Mumbai, India). Briefly, A549 cells (3 × 104 cells/well) were seeded in 96-well plates and treated for 24 h with various concentrations of Coronil (1–1000 µg/mL). Two hours before termination, 10 µL of Alamar blue (0.15 mg/mL) was added to each well. Cytotoxicity was measured by reading fluorescence at Ex. 540/Em. 590 using the Envision microplate reader (Perkin Elmer, Waltham, MA, USA).
Alamar blue reagent
Manufactured by HiMedia
Sourced in India
Alamar Blue reagent is a commonly used cell viability indicator. It measures the reducing environment of the living cells, providing a quantitative measure of cellular metabolic activity.
Automatically generated - may contain errors
Lab products found in correlation
Envision microplate reader, by PerkinElmer (3 mentions)
Middlebrook 7h9, by HiMedia (1 mentions)
Polyvinyl alcohol (pva), by HiMedia (1 mentions)
Resazurin sodium salt, by HiMedia (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Powerup sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Mitotracker red fm, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Collagenase type 2, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Verso cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Cyquant ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions)
Cellrox green reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Histopaque, by Merck Group (1 mentions)
Pioglitazone, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Lipidspot lipid droplet stain, by Biotium (1 mentions)
Trypsin phosphate versene glucose solution, by HiMedia (1 mentions)
Methylcellulose, by Loba Chemie (1 mentions)
7 protocols using alamar blue reagent
1
Evaluating Coronil's Impact on A549 Cell Viability
2
Antitubercular Activity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MIC values were determined using the MABA; rifampicin and isoniazid were employed as references. The mycobacteria strain has been cultured at 37 °C for two weeks in Middlebrook 7H9 (Himedia, Mumbai, India) supplemented with 0.05% (v/v), 2% glycerol and 10% OADC (oleic acid–albumin–dextrose–catalase of Liofilchem s.r.l, Roseto degli Abruzzi, Italy). The 96-well plates received 100 μL of Middlebrook 7H9 broth and serial dilution of compounds was made directly on the plate with drug concentrations of 0.244–250 μg/mL and 5000 to 4.882 μg/mL for extracts. Plates were covered and sealed with parafilm and incubated at 37 °C for 14 days. Then, 40 μL of freshly prepared 1:1 mixture of alamar blue reagent and 7% Tween 80 (Himedia, Mumbai, India) was added to the plate and incubated for 24 h. A blue colour in the well was interpreted as no bacterial growth and pink colour was scored as growth. The MIC was defined as the lowest drug concentration, which prevented colour change from blue to pink. The results of antitubercular activity are depicted in Tables 2 and 3 . Minimum inhibitory concentration (MIC) was determined by the broth micro-dilution method according to the guidelines of the Clinical and Laboratory Standards Institute (2011 ).
3
Evaluating Cytotoxicity of Denguenil Vati
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2 and A431 cell viabilities were measured using Alamar blue reagent (Hi Media, India). Briefly, both HepG2 and A431 cells (2 × 104 cells/well) were seeded in 96-well plates and treated for 24 h with various concentrations of Denguenil Vati (1–1000 µg/mL). Two hours before termination, 10 µL of Alamar blue (0.15 mg/mL) was added to each well. Cytotoxicity was measured by reading fluorescence at Ex. 540/Em. 590, using an Envision microplate reader (Perkin Elmer, Waltham, MA, USA).
4
Colorimetric Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PVA, CMC and resazurin sodium salt (alamarBlue reagent) were obtained from HiMedia Laboratories Pvt Ltd. All chemicals and reagents used were of analytical grade.
5
Livogrit Characterization and Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Livogrit (Batch #ALGT200001) was obtained from Divya Pharmacy (Haridwar, Uttarakhand, India). Dulbecco′s Modified Eagle′s Medium (DMEM), DMEM without L-methionine and L-cystine, pioglitazone, Histopaque and Corning 96 well black clear bottom polystyrene microplates were procured from Sigma–Aldrich (St. Louis, MO, USA). Penicillin-Streptomycin and collagenase type II were obtained from Gibco (Waltham, MA, USA). Trypsin Phosphate Versene Glucose (TPVG) solution, Fetal Bovine Serum (FBS), N-Cetyl-N,N,N-trimethyl ammonium bromide (CTAB), Nile red, and Alamar Blue reagent were purchased from HiMedia (Mumbai, India). Methylcellulose was obtained from Loba Chemie (Mumbai, India). AST and glycerol detection kits were obtained from Randox Laboratories Ltd. (Crumlin, UK). RNeasy mini kit was purchased from Qiagen (Hilden, Germany). The PowerUp SYBR Green Master Mix was procured from Applied Biosystems (Foster City, CA, USA). LipidSpot lipid droplet stain was obtained from Biotium (Fremont, CA, USA). CyQUANT LDH cytotoxicity assay kit, Verso cDNA synthesis kit, protease inhibitor cocktail, CellROX Green reagent, and MitoTracker Red FM were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
6
Cytotoxicity Assay of Repsox
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293T, HT1080, and A549 cells were seeded in 96-well plates (10,000/well) and co-transfected with plasmids encoding Cas9 (50 ng) and guide RNA (50 ng) using Lipofectamine 3000. Cells were treated with Repsox at different concentrations (0.1, 0.5, 1, 5, 10, 20, and 50 μM) for 48 h. After 48 h of compound addition, Alamar Blue reagent (HiMedia) was added to each well according to the manufacturer’s protocol, and the plate was incubated at 37°C for 4 h. The absorbance was measured at 570 nm using a SpectraMaxi3X plate reader.
7
Cytotoxicity Evaluation of Divya-Swasari-Vati
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell viability assay was performed under standard conditions using Alamar Blue reagent (HiMedia Laboratories Pvt. Ltd., Mumbai, Maharashtra, India). In brief, 3x104 cells were seeded in a 96 well plate, in triplicate, and incubated in the presence of different concentrations of Divya-Swasari-Vati (0.1, 0.3, 1, 3, 10, 30 and 100 µg/mL) for a period of 24 hours. Two hours before termination, 10 µL of Alamar Blue (0.15 mg/mL) was added to each well and the cell cytotoxicity was measured at an emission of 590 nm with 540 nm excitation, using an Envision microplate reader (PerkinElmer, Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!