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Ez run rec protein ladder

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The EZ-Run Rec Protein Ladder is a molecular weight standard used for estimating the size of proteins in polyacrylamide gel electrophoresis (PAGE) experiments. It contains a mixture of recombinant proteins with known molecular weights, allowing for the determination of the approximate molecular weights of unknown protein samples.

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Lab products found in correlation

Nupage novex, by Thermo Fisher Scientific (7 mentions) Coomassie protein assay reagent, by Thermo Fisher Scientific (5 mentions) Simplyblue safestain, by Thermo Fisher Scientific (5 mentions) Mops running buffer, by Thermo Fisher Scientific (4 mentions) Vector nti advance, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Bioreagents EZ-run prestained rec protein ladder EZ-Run™ Prestained Rec Protein Ladder EZ-Run protein ladder EZ-Run Protein ladder

7 protocols using ez run rec protein ladder

1

Protein Visualization and Quantification

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The proteins in each fraction were visualized by sodium dodecyl sulfate–polyacrylamide gel electrophoresis using 4% to 12% polyacrylamide premade gradient gels (Novex NuPAGE; Invitrogen, Grand Island, NY) with 3-(N-morpholino)propanesulfonic acid as the running buffer (Invitrogen) followed by staining with Coomassie. EZ-Run Rec Protein Ladder (Fisher Scientific) was used as a protein standard. Coomassie Protein Assay Reagent (Thermo Scientific, Waltham, MA) was used to determine protein concentrations with bovine serum albumin as a standard.
Balboa S., Hu Y., Dean F.B, & Bullard J.M. (2019). Lysyl-tRNA Synthetase from Pseudomonas aeruginosa: Characterization and Identification of Inhibitory Compounds. SLAS discovery : advancing life sciences R & D, 25(1), 57-69.
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2

Protein Quantification and Alignment

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SDS-PAGE was performed using 4-12% polyacrylamide pre-cast gels (Novex NuPAGE; Invitrogen) with MOPS running buffer (Invitrogen). EZ-Run™ Rec Protein Ladder was from Fisher. Gels were stained with SimplyBlue Safe Stain (Invitrogen). Protein concentrations were determined using protocols from Bradford [22 (link)] using bovine serum albumin as the standard. Protein alignments were prepared using Vector NTI Advance ® (Invitrogen).
Hu Y., Palmer S.O., Munoz H, & Bullard J.M. (2015). High Throughput Screen Identifies Natural Product Inhibitor of Phenylalanyl-tRNA Synthetase from Pseudomonas aeruginosa and Streptococcus pneumoniae. Current drug discovery technologies, 11(4), 279-292.
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3

SDS-PAGE Analysis of Protein Samples

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SDS-PAGE was performed using 4–12% polyacrylamide pre-cast gels (Novex NuPAGE; Invitrogen) with MOPS running buffer (Invitrogen). EZ-Run™ Rec Protein Ladder was from Fisher Scientific. Gels were stained with Simply Blue Safe Stain (Invitrogen). Protein concentrations were determined using Coomassie Protein Assay Reagent (Thermo Scientific) with bovine serum albumin as the standard.
Zamacona R., Chavero P.N., Medellin E., Hu Y., Hughes C.A., Quach N., Keniry M, & Bullard J.M. (2018). Identification and Characterization of Chemical Compounds that Inhibit Leucyl-tRNA Synthetase from Pseudomonas aeruginosa. Current drug discovery technologies.
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4

SDS-PAGE Protein Separation and Quantification

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Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed using 4–12% polyacrylamide precast gels (Novex NuPAGE; Invitrogen, Grand Island, NY) with 3-(N-morpholino)propanesulfonic acid (MOPS) running buffer (Invitrogen). EZ-Run Rec Protein Ladder was from Fisher Scientific. Gels were stained with Simply Blue Safe Stain (Invitrogen). Protein concentrations were determined using Coomassie Protein Assay Reagent (Thermo Scientific, Waltham, MA) with bovine serum albumin as the standard.
Hu Y., Guerrero E., Keniry M., Manrrique J, & Bullard J.M. (2015). Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa. Journal of biomolecular screening, 20(9), 1160-1170.
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5

SDS-PAGE Protein Visualization Protocol

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Proteins were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4–12% polyacrylamide precast gradient gels (Novex NuPAGE; Invitrogen, Grand Island, NY) with 3-(N-morpholino) propanesulfonic acid (MOPS) as the running buffer (Invitrogen). EZ-Run Rec Protein Ladder (Fisher Scientific) was used as a protein standard. Coomassie Protein Assay Reagent (Thermo Scientific, Waltham, MA) was used to determine protein concentrations with bovine serum albumin as a standard [8 (link)].
Robles S., Hu Y., Resto T., Dean F, & Bullard J.M. (2017). Identification and Characterization of a Chemical Compound that Inhibits Methionyl-tRNA Synthetase from Pseudomonas aeruginosa. Current drug discovery technologies, 14(3), 156-168.
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6

SDS-PAGE Protein Analysis

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Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed using 4%–12% polyacrylamide precast gels (Novex NuPAGE, Invitrogen, Waltham, MA) with MOPS running buffer (Invitrogen). EZ-Run Rec Protein Ladder was from Fisher Scientific. Gels were stained with Simply Blue Safe Stain (Invitrogen). Protein concentrations were determined using Coomassie Protein Assay Reagent (Thermo Scientific, Waltham, MA) with bovine serum albumin as the standard.
Corona A., Palmer S.O., Zamacona R., Mendez B., Dean F.B, & Bullard J.M. (2017). Discovery and Characterization of Chemical Compounds That Inhibit the Function of Aspartyl-tRNA Synthetase from Pseudomonas aeruginosa. SLAS discovery : advancing life sciences R & D, 23(3), 294-301.
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7

SDS-PAGE Protein Separation and Analysis

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Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed using 4%–12% polyacrylamide precast gels (Novex NuPAGE; Invitrogen, Waltham, MA) with MOPS running buffer (Invitrogen). EZ-Run Rec Protein Ladder was from Fisher Scientific. Gels were stained with Simply Blue Safe Stain (Invitrogen). Protein concentrations were determined by the method of Bradford14 (link) using bovine serum albumin (BSA) as the standard.
Hu Y., Palmer S.O., Robles S.T., Resto T., Dean F.B, & Bullard J.M. (2017). Identification of Chemical Compounds That Inhibit the Function of Histidyl-tRNA Synthetase from Pseudomonas aeruginosa. SLAS discovery : advancing life sciences R & D, 23(1), 65-75.
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