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8 protocols using tetrachloroauric iii acid trihydrate haucl4 3h2o

1

Graphene-based Colorimetric Biosensor

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Tetrachloroauric (III) acid trihydrate (HAuCl4 3H2O, Sigma-Aldrich, St. Louis, MO, USA), trisodium citrate (Na3C6H5O7, Sigma-Aldrich, St. Louis, MO, USA), and GO (Graphene Supermarket, Calverton, NY, USA) were used as purchased. Bst DNA polymerase, 10X of ThermoPol buffer, 100 mM magnesium sulfate (MgSO4), and 10 mM dNTP were obtained from New England BioLabs (Ipswich, MA, USA). SYBR green I fluorescent nucleic acid was purchased from LONZA (Rockland, ME, USA). Ultrapure water was used throughout this study.
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2

Electrochemical Detection of BPA

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Tetrachloroauric (III) acid trihydrate (HAuCl4·3H2O), BPA, HCl, NaOH, Na2HPO4, NaH2PO4, and all interference compounds were purchased from Sigma-Aldrich. Carboxylated multi-walled carbon nanotubes were acquired from Orion High Technologies S.L. Screen-printed carbon (DRP-110) (φ~0.13 cm2) and glassy carbon electrodes (φ~0.07 cm2) were purchased from DropSens and CH Instruments Inc., respectively. SPCE consisted of a three-electrode system, in which working (4 mm diameter) and counter electrodes are made of carbon and the pseudo-reference electrode is Ag/AgCl. For experiments with GCEs, a platinum wire was used as the counter electrode, meanwhile, an Ag/AgCl (3M KCl) electrode was used as the reference electrode.
Commercial water samples were acquired from local markets and tap water from local suppliers. Carboxylated CNTs (10 mg) were dispersed in 10 ml of water and subjected to sonication for one hour. Before using, CNT solution was sonicated for 1 min. Then, gold nanoparticle precursor solution was obtained dissolving HAuCl4·3H2O in double-distilled water at a concentration of 3 mM. Both solutions were conserved in the fridge at 4 °C before use.
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3

Gold Nanoparticles from Turkish Plants

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Analytical grade Tetrachloroauric (III) acid trihydrate (HAuCl4·3H2O) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Plants were purchased from the local market in Türkiye and used after the biological identification by a plant taxonomist at the Department of Botany, Erciyes University, Turkey.
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4

Synthesis and Catalytic Applications

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Catalytic activity on reduction reaction was evaluated from the transformation of 4-nitrophenol (p-NP, 99%) supplied by Sigma-Aldrich. Selective oxidation catalysis was evaluated from the oxidation of dimethyl(phenyl)silane (C8H12Si, 98%) supplied by Sigma-Aldrich. Magnetic cobalt ferrite was synthesized using ACS reagent quality iron(ii) chloride anhydrous and cobalt chloride hexa-hydrated purchased from Sigma-Aldrich and Merck, respectively. Gold nanoparticles were synthesized using tetrachloroauric(iii) acid trihydrate (HAuCl4·3H2O, ≥99%) supplied by Sigma-Aldrich. Other chemicals used in nano-synthesis processes such as trisodium citrate di-hydrate, tannic acid, potassium carbonate, methylamine (40% w/w), (3-aminopropyl)triethoxysilane (APTES), and sodium dodecyl sulfate (SDS) were of analytical grade supplied by Sigma-Aldrich and Merck. Reductant agent sodium borohydride was supplied by Merck. All solutions were prepared with ultrapure water obtained from a Millipore Milli-Q system with resistivity > 18.2 MΩ cm at 25 °C.
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5

Synthesis and Characterization of Gold Nanorods

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Freshly bidistilled water was used as a solvent in all the procedures. All reactants required were purchased and used as received, without further purification: cetyltrimethylammonium bromide (C19H42BrN, CTAB, ≥97% Millipore Corporation), tetrachloroauric(iii) acid trihydrate (HAuCl4·3H2O, ≥99.9%, Sigma-Aldrich), sodium borohydride (NaBH4, 99.99%, Sigma-Aldrich), silver nitrate (AgNO3, 99.9%, Carlo Erba), l-ascorbic acid (C6H8O6, 99%, Sigma-Aldrich) and hydroquinone (C6H602, ≥99%, Sigma-Aldrich). A mini spin Eppendorf centrifuge was used for the purification of AuNR samples. Uv-visible spectra were acquired with a Shimadzu 2401 PC spectrophotometer (200–800 nm), while NIR spectra were measured with a Nicolet IS50 FT-IR Thermo scientific spectrophotometer (400–1100 nm) using quartz cells with a 1 cm optical path.
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6

Synthesis and Characterization of Hybrid Microgels

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Styrene (STY), N-isopropylmethacrylamide (NIPM) (98%), N,N-methylenebisacrylamide (MBIS) (99%), ammonium per sulfate (APs) (99%), sodium dodecyl sulfate (SDS) (98%), sodium borohydride (NaBH4) (98%), 2-nitrophenol (2NP), 4-nitrophenol (4NP), 3-nitrophenol (3NP), 4-chloronitrobenzene (4NCB), 2-nitroaniline (2NA), 3-nitroaniline (3NA), 4-nitroaniline (4NA), 2-amino-4-nitroanisole (2A4NA), 4-nitrophenylhydrazine (4NPH), 2,4-dinitrophenylhydrazine (2,4DNPH), and tetrachloroauric(iii) acid trihydrate (HAuCl4·3H2O) were obtained from Sigma Aldrich (Germany). No further purification of the chemicals was done except STY. STY was purified via vacuum filtration by passing it through alumina (Al2O3) to remove the inhibitor. Deionized water was used in all the experimental work. The membrane for dialysis (cut off = 12 000–14 000) was obtained from Scharlu and was used for the removal of unreacted reactants from the microgel and hybrid microgel dispersions.
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7

Synthesis of Gold Nanoparticles

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Tetrachloroauric (III) acid trihydrate (HAuCl4 ⋅ 3 H2O) was obtained from Sigma Aldrich (99.9 %). 1‐Ethyl‐3‐methylimidazolium dicyanamide ([Emim][DCA], >98 %) was purchased from Iolitec Ionic Liquids Technologies GmbH. All chemicals were used as received without further purification.
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8

Synthesis of Metal Nanoparticles and SERS Substrates

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All chemicals were used without any further treatment: G5-NH2 PAMAM dendrimers (Dendritech, Midland, MI, USA); Tetrachloroauric(III) acid trihydrate (HAuCl4•3H2O, 99.9%, Sigma-Aldrich); Silver nitrate (AgNO3, Sigma-Aldrich, >99.0%); sodium dodecyl sulfate salt (SDS, NaC12H25SO4, Sigma-Aldrich, >98.0%); α,α'-azobis-(isobutyronitrile) (AIBN, C8H12N4, Fluka >98.0%); hexadecane (C16H34, Sigma-Aldrich, >99.0%), glycerol (C3H8O3, Sigma-Aldrich, >99.0%); sodium bicarbonate (NaHCO3, Sigma-Aldrich, >99.5%) Thiram (C6H12N2S4, Sigma-Aldrich, ≥98.0%); Styrene (C8H8, Aldrich 99%) was purified over a column of neutral Al2O3 and stored at 4 °C. Colloids of gold, silver and their alloys were prepared using ultrapure water (18.2 MΩ•cm, 25 °C, MilliQ, Millipore); A4 office paper sheets with a grammage of 80 g m -2 (Navigator, Portugal) were used for the preparation of the SERS substrates.
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