Mithramycin

Manufactured by Bio-Techne

Mithramycin is a laboratory reagent used for the detection and quantification of guanine-cytosine (GC)-rich DNA sequences. It is a fluorescent dye that binds specifically to GC-rich regions of DNA, emitting a fluorescent signal when exposed to ultraviolet light. Mithramycin can be used in various applications, such as nucleic acid quantification, gene expression analysis, and DNA-binding studies.

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Lab products found in correlation

Pha 767491, by Selleck Chemicals (1 mentions) Small molecule inhibitors, by Selleck Chemicals (1 mentions) Pha 767491, by Adooq (1 mentions) D α tocopherol polyethylene 1000 succinate, by Merck Group (1 mentions) Gadolinium, by Merck Group (1 mentions) Sp600125, by Bio-Techne (1 mentions) Sr11302, by Bio-Techne (1 mentions) Nsc23766, by Bio-Techne (1 mentions) Anti pax9, by Abcam (1 mentions) L name, by Merck Group (1 mentions) Anti laminin, by Abcam (1 mentions) Anti tnc, by Abcam (1 mentions) Sb203580, by Bio-Techne (1 mentions) Anti lox, by Abcam (1 mentions) Cytochalasin d, by Bio-Techne (1 mentions) U0126, by Bio-Techne (1 mentions) Sb431542, by Bio-Techne (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

2 protocols using mithramycin

Preparation and Storage of Mithramycin and PHA-767491

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Mithramycin (Tocris Bioscience) was dissolved as a 1 mg/mL stock solution in PBS, and frozen in aliquots. PHA-767491 and other small molecule inhibitors (Selleck Chemicals) were dissolved in DMSO as stock solutions. Small molecule stocks were stored at −80 °C for storage or −20 °C for experimental use, minimizing freeze-thaw cycles. For in vivo experiments, a 30 mg/mL stock solution of PHA-767491 (Adooq Bioscience) was made by dissolving the powder in a solution of 1 mg/10mL D-α-Tocopherol polyethylene 1000 succinate (MilliporeSigma) for oral gavage.

Flores G., Everett J.H., Boguslawski E.A., Oswald B.M., Madaj Z.B., Beddows I., Dikalov S., Adams M., Klumpp-Thomas C.A., Kitchen-Goosen S.M., Martin S.E., Caplen N.J., Helman L.J, & Grohar P.J. (2020). CDK9 Blockade Exploits Context-Dependent Transcriptional Changes to Improve Activity and Limit Toxicity of Mithramycin for Ewing Sarcoma. Molecular cancer therapeutics, 19(5), 1183-1196.

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Cell Signaling Modulators in ECM Interactions

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Gadolinium, anti N-cadherin neutralizing antibody, L-name, ML7, PMA and BAPN were from Sigma; anti integrin blocking antibody, Anti-collagen I, III, IV and VI, anti-TNC, anti-laminin, anti-biglycan, anti-decorin, anti-lumican, anti-LOX and anti-Pax9 antibodies were from Abcam; C3 and CN02 were from Cytoskeleton Inc; Y27632 and LY294002 were from Calbiochem; pertussis toxin, NSC23766, cytochalasin D, H89, C1, U0126, SB203580, SP600125, PP1, SB431542, SR11302 and mithramycin were from Tocris; and cytotoxic necrotizing factor 1 (CNF 1) was prepared as described previously (Mammoto et al., 2004 (link)).

Mammoto T., Mammoto A., Jiang A., Jiang E., Hashmi B, & Ingber D.E. (2015). Mesenchymal condensation-dependent accumulation of collagen VI stabilizes organ-specific cell fates during embryonic tooth formation. Developmental dynamics : an official publication of the American Association of Anatomists, 244(6), 713-723.

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