Human c reactive protein crp quantikine elisa kit

Before operation and 1 week after operation, 5 mL of fasting peripheral venous blood was collected from all aSAH patients, and centrifuged at 3,000 rpm for 10 min. Then the supernatant was collected, and S100B, monocyte chemotactic protein-1 (MCP-1) and C-reactive protein (CRP) were detected using Human S100B Duo Set ELISA (DY1820-05, R&D Systems, USA), Human CCL2/ MCP-1 Quantikine ELISA Kit (DCP00, R&D Systems, USA) and Human C-Reactive Protein/CRP Quantikine ELISA Kit (DCRP00, R&D Systems, USA) strictly according to the instructions.

The level of soluble E-selectin (sE-selectin) was measured using Human E-selectin enzyme-linked immunosorbent assay (ELISA) kit (Multi Sciences, China) following the manufacturer’s instructions. The level of the plasma C-reactive protein (CRP) from human subjects was analyzed using a Human C-Reactive Protein/CRP Quantikine ELISA Kit (R&D, United States) following the manufacturer’s instructions. The absorbance was read at 450 nm and 570 nm using the Epoch Microplate Spectrophotometer (BioTek, United States).

Plasma levels of the iron transporter ferritin and the iron regulator hepcidin as well as those of C-reactive protein (CRP) and interleukin (IL) 6 were measured using enzyme-linked immunosorbent assays (ferritin: Human ELISA Kit, Thermo Scientific (Waltham, MA, USA); hepcidin: Intrinsic Hepcidin IDx™ ELISA Kit, Intrinsic LifeSciences (La Jolla, CA, USA); CRP: Human C-Reactive Protein/CRP Quantikine ELISA Kit, R&D Systems (Minneapolis, MN, USA); IL6: Human IL-6 Quantikine HS ELISA Kit, R&D Systems). Plate processing and data collection were carried out according to the manufacturer’s instructions. Absorbance was read on a Synergy HT Multi-Detection microplate reader (BioTek, Winooski, VT, USA). Concentrations of ferritin, hepcidin, and CRP are shown as ng/mL, whilst IL6 levels are reported in pg/mL.

Serum specimens were stored at −80 °C until the measurements. We measured hsCRP using a quantitative enzyme-linked immunosorbent assay with a sensitivity of 25 ng/L (Human C-Reactive Protein/CRP Quantikine ELISA Kit, R&D Systems, Inc., Minneapolis, MN, USA). According to the manufacturer’s instructions, the samples were assayed in duplicate and the absorbance was measured at 450 nm. The final concentrations were calculated from the respective standard curves and expressed as mg/L.

The inflammatory biomarkers IL6, IL10, CRP, and TTP were examined to evaluate the effects of the intervention on inflammation in DFUs. Evaluation of IL6 and CRP was undertaken using a human C-Reactive Protein/CRP Quantikine ELISA Kit and a human IL6 Quantikine ELISA Kit from R&D systems (Biotechne, Minneapolis, MN, USA). For CRP, the mean CV% for intra-assay precision was 5.5% and for inter-assay precision was 6.5%. The reported mean CV% for IL6 for intra-assay precision was 2.6% and for inter-assay precision was 4.5%. Assessments of IL10 and TTP were conducted using human ELISA kits from MyBioSource (San Diego, CA, USA). The mean CV% for intra-assay and inter-assay for IL10 were 4.44% and 6.27%, respectively, and for TTP were ≤8% and ≤12%, respectively. We were not able to read the data following standard instructions for IL10 and TTP ELISA tests, since our population had an exceptionally low concentration of these biomarkers. After consulting with the company’s technical support, when evaluating IL10 using ELISA kits the first incubation time was increased to 2 h and the plate was put in a slow shaker during incubation. We also performed the test with standard curve assayed in serum diluent, and samples assayed and incubated at 4 °C overnight (20 h). No other changes were made to other procedures and protocols.