Peripheral blood and lymph node samples from leukemia patients (Supplementary table 2 ) were obtained after written informed consent and with the approval from the Institutional Research Ethics Committee of respective institutions and Declaration of Helsinki. The approved institutions are Stanford University School of Medicine, KaviKrishna Telemedicine Care, a branch of KaviKrishna laboratory, Dr. B. Borooah Cancer Institute and Gauhati University. The collection and expansion of T-cell lymphoblastic lymphoma (T-LBL) samples (n=6, Supplementary table 2 ), immunomagnetic sorting of ABCG2+ and in vivo transplantation in non-obese diabetic/severe combined immune deficient (NOD/SCID) mice were performed as described (20 (link), 43 (link), 44 (link)). Mice were prior treated with 22 mg/kg intraperitoneal Busulfan (6mg/ml injection, Taj Pharma, Mumbai) daily over three days (20 (link)). The human cell engraftment was confirmed by flow cytometry staining with a human CD45 antibody (Biolegend, CA).
Human cd45 antibody
Manufactured by BioLegend
The Human CD45 Antibody is a laboratory reagent used for the identification and quantification of CD45-expressing cells. CD45 is a transmembrane protein tyrosine phosphatase that is expressed on the surface of all nucleated hematopoietic cells. This antibody can be used in various applications such as flow cytometry and immunohistochemistry to study the distribution and phenotype of CD45-positive cells.
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Lab products found in correlation
2 protocols using human cd45 antibody
1
Engrafting T-LBL Cells in NOD/SCID Mice
2
Engraft Jurkat Cells for Leukemia Study
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Use of mice was approved by the University of Kentucky’s IACUC, protocol 2017–2754. Eight-week old NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). Eight mice per group were used for experiments based on pilot studies that utilized three mice per group. The mice were randomized by placing into groups such that the difference between average group weight is not greater than 10%. Jurkat cells were infected with Scrambled shRNA or PRL-3 shRNA as described above. Two days after virus infection, Jurkat cells were selected using 5 μg/ml puromycin for two days, stained with trypan blue, and viable cells were FACS isolated. 106 live cells in 100 μL PBS were injected intravenously. Peripheral blood samples (100–150 μL) were collected by submandibular bleeding at 4, 6, and 8 weeks post-transplantation and stained with human CD45 antibody according to Biolegend’s protocol and analyzed by flow cytometry.
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