Super Q (80/100 mesh) was purchased from Alltech (PA, USA). n-Hexane (95%, for pesticide residue analysis) was purchased from Fisher Scientific (Loughborough, Leicestershire, UK). (Z)-3-Hexenyl acetate (98%) were purchased from Alfa Aesar (Heysham, UK). Limonene (97%), cis-jasmone (92%) and linalool (96%) were purchased from TCI America (Portland, USA). α-Pinene (98%), camphene (90%), 6-methyl-5-hepten-2-one (98%), methyl salicylate (99%), ocimene (90%) (mix of (E) and (Z)-isomers), (E)-caryophyllene (80%), benzothiazole (96%), indole (98%), myrcene (97%), octanal (99%), undecane (99%), tridecane (99%), pentadecane (99%), nonanal (95%) and decanal (98%) were purchased from Sigma Aldrich (Steinheim, Germany). Geranylacetone (96%) was purchased from TCI (Tokyo, Japan). (E,E)-α-Farnesene was synthesized in three steps from isoprene and sulfur dioxide (Hassemer et al. 2016 (link)). (E)-4,8-Dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) were synthesized from geraniol and (E,E)-farnesol, respectively (Leopold 1990 ).
E caryophyllene
Manufactured by Merck Group
Sourced in Germany, Italy, United States, France
(E)-caryophyllene is a natural organic compound that can be used as a reference standard for analytical and research purposes. It is a bicyclic sesquiterpene with the chemical formula C₁₅H₂₄. The compound is commonly extracted from essential oils of various plants.
Automatically generated - may contain errors
Lab products found in correlation
α pinene, by Merck Group (9 mentions)
β pinene, by Merck Group (8 mentions)
α humulene, by Merck Group (8 mentions)
Limonene, by Merck Group (7 mentions)
P cymene, by Merck Group (7 mentions)
Camphene, by Merck Group (6 mentions)
γ terpinene, by Merck Group (6 mentions)
Myrcene, by Merck Group (5 mentions)
Linalool, by Merck Group (4 mentions)
α terpineol, by Merck Group (4 mentions)
Caryophyllene oxide, by Merck Group (4 mentions)
1 8 cineole, by Merck Group (4 mentions)
Sabinene, by Merck Group (4 mentions)
Terpinolene, by Merck Group (4 mentions)
α phellandrene, by Merck Group (4 mentions)
Nonanal, by Merck Group (3 mentions)
Decanal, by Merck Group (3 mentions)
Pentadecane, by Merck Group (3 mentions)
δ 3 carene, by Merck Group (3 mentions)
Terpinen 4 ol, by Merck Group (3 mentions)
15 protocols using e caryophyllene
1
Synthesis of Volatile Organic Compounds
2
Identification of Volatile Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the identification of volatiles, the following analytical standards purchased from Sigma Aldrich (Milan, Italy) were used: α-pinene, β-pinene, sabinene, 1,8-cineole, camphene, myrcene, α-phellandrene, δ-3-carene, p-cymene, limonene, γ-terpinene, terpinolene, linalool, trans-pinocarveol, terpinen-4-ol, α-terpineol, myrtenal, citronellol, isobornyl acetate, (E)-caryophyllene, α-humulene, (E)-β-ionone, and caryophyllene oxide. The reference drug suramin was purchased from Sigma Aldrich.
3
Volatile Identification via GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For identification of volatiles the following analytical standards were purchased from Sigma-Aldrich (Milan, Italy): α-pinene, β-pinene, p-cymene, limonene, γ-terpinene, linalool, trans-pinocarveol, terpinen-4-ol, α-terpineol, myrtenol, nerol, carvone, geraniol, geranial, eugenol, (E)-caryophyllene, α-humulene, (E)-β-farnesene, (E)-β-ionone, (E)-nerolidol, caryophyllene oxide, n-pentacosane, n-heptacosane; (E)-Phytol was previously isolated from Onosma echioides [43 (link)]. For retention-index (RI) determination, a mixture of hydrocarbons, ranging from octane (C8) to triacontane (C30) (Supelco, Bellefonte, PA, USA) was used and run under the experimental conditions reported below. All compounds were of analytical standard grade. Analytical-grade hexane solvent was purchased from Carlo Erba (Milan, Italy); it was successively distilled through a Vigreux column before use.
4
Essential Oil Extraction and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
Casearia sylvestris essential oil was obtained by
hydrodistillation of its leaves (150 g) for 4 h in a Clevenger-type apparatus
according to the procedure described in the European Pharmacopoeia21 . M. divaricatum essential oil was obtained by Moreira
et al.16 (link). The EOs were stored in amber bottles and kept in the refrigerator at 4
°C until further analysis. The yields (w/w) were calculated from the weight of
the fresh aerial parts and leaves of M. divaricatum (0.4%) and
leaves of C. sylvestris (0.3%).
E-Caryophyllene was purchased from Sigma-Aldrich Co. (St.
Louis, MO, USA).
+ Expand
Casearia sylvestris essential oil was obtained by
hydrodistillation of its leaves (150 g) for 4 h in a Clevenger-type apparatus
according to the procedure described in the European Pharmacopoeia21 . M. divaricatum essential oil was obtained by Moreira
et al.16 (link). The EOs were stored in amber bottles and kept in the refrigerator at 4
°C until further analysis. The yields (w/w) were calculated from the weight of
the fresh aerial parts and leaves of M. divaricatum (0.4%) and
leaves of C. sylvestris (0.3%).
E-Caryophyllene was purchased from Sigma-Aldrich Co. (St.
Louis, MO, USA).
5
Extraction and Characterization of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals used for this study, dimethylsulfoxide (DMSO), β-pinene, limonene, (E)-caryophyllene, α-humulene, caryophyllene oxide, dichloromethane, and permethrin, were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.
6
Characterization of Perfume Raw Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Camphor (96%), carvacrol (99%), (L)-carvone (99%), E-caryophyllene (≥98%), citronellol (≥95%), eucalyptol (99%), eugenol (≥98%), geraniol (≥97%), R-(+)-limonene (≥98%), (AE)-linalool (>97%), (AE)-menthol (≥98%), (-)-menthone (96%), α-pinene (98%), tonalide (≥98%), vanillin (99%) and Tween ® 20 were all obtained from Sigma-Aldrich. Ethanol (P.A. 99.8%), 1-propanol (67-63-0 ≥ 99.5%), mEthanol (67-56-1 P.A. 99.9%), phosphate buffer solution (pH 7.4) and glacial acetic acid (64-19-7) were obtained from Merck. The perfume raw materials are listed in Table 1 with their chemical structure presented in Fig. 1. All the PRMs are miscible with Ethanol (Hazardous Substances Data Bank (HSDB) and Joint FAO/WHO Expert Committee on Food Additives (JECFA)), with exception of vanillin and Camphor solubilities, 50 mg mL -1 (Sigma ® product datasheet) and 1 g mL -1 (HSDB).
7
Qualitative Analysis of Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The qualitative analysis was carried out on the basis of MS spectra which were compared with the spectra library by means of the NIST MS Search Program (NIST 08, Software Version 2.0f) [7 ], and with the data available in literature [8 , 9 ]. Identity of the compounds was confirmed by their retention indices [10 (link)], taken from literature [8 , 9 ], and our own data for standards (1,8-cineole, chamazulene, eugenol, limonene, menthone, thymol, carvone, carvacrol, menthol, caryophyllene oxide, E-caryophyllene from Fluka, Sigma-Aldrich Chemie GmbH, Germany).
8
Odorant Compounds Sourcing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diethyl ether (99.5%) was purchased from Fisher Scientific, UK. Allyl isothiocyanate (95%), (Z)-3-hexen-1-ol (98%), 1-octen-3-one (96%), nonanal (95%), (E)-anethol (99%), (S)-bornyl acetate (95%), (E)-caryophyllene (98%), pentadecane (>99%) were from Sigma-Aldrich, UK.
9
Clary Sage Essential Oil Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clary sage (Salvia sclarea) essential oil was purchased from the Slovak company Hanus s.r.o. (Nitra, Slovakia). The essential oil was obtained by steam distillation of young branches and leaves of Salvia sclarea by the producer. The standard compound, (E)-caryophyllene, ≥98.5% was purchased from Sigma-Aldrich (Taufkirchen, Germany).
10
GC-MS Analysis of Flavor Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemicals used in GC-MS analysis including hexanal, heptanal, benzaldehyde, octanal, nonanal, decanal, 6-methyl-5-hepten-2-one, acetophenone, α-phellandrene, α-pinene, p-cymene, β-citronellene, camphene, sabinene, β-pinene, limonene, 1,8-cineole, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, δ-2- carene, (Z)-linalool oxide (furanoid), linalool, α-copaene, α-cedrene, (E)-caryophyllene, α-humulene, (Z)- caryophyllene, skatole, heptanol, octanol, 1-octen-3-ol, nonanol, p-xylene, o-xylene, phenol, benzyl alcohol, m-cresol, p-cresol, indole, and standard n-alkanes solution were purchased from Sigma Aldrich (purity >97%).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!