Pe conjugated anti mouse il 17

BV-510 conjugated anti-mouse CD3 (145-2C11), PerCP-Cy5.5 conjugated anti-mouse CD4 (RM4-5), APC-cy7 conjugated anti-mouse CD8 (53-6.7), FITC conjugated anti-mouse γδTCR (GL3), PE-CD25 conjugated anti-mouse (A7R34), APC-conjugated anti-mouse CD69 (H1.2F3), APC-conjugated anti-mouse CD44 (IM7), PE-cy7 conjugated anti-mouse CD278 (ICOS, 15F9), Percp5.5 conjugated anti-mouse CD16/32 (93), APC conjugated anti-mouse IFN-γ (XMG1.2), PE conjugated anti-mouse IL-4 (11B11), PE conjugated anti-mouse IL-17 (TC11-18H10), PE conjugated anti-mouse IL-10 (JES5-16E3), APC conjugated anti-mouse IL-5(TRFK5), PE conjugated anti-mouse IL-2 (561061), PE conjugated anti-mouse CD154 (MR1), PE conjugated anti-mouse CD183 (CXCR3-173), PE conjugated anti-mouse CD80 (16-10A1), matched control monoclonal antibodies (MG1-45) were purchased from BioLegend (San Diego, CA, USA).

Recombinant mouse IL-6, purified anti-mouse CD28 mAb, anti-mouse CD3 mAb, FITC conjugated anti-mouse CD4, APC-conjugated anti-mouse IFN-γ, and PE-conjugated anti-mouse IL-17, anti-mouse IL-4 mAb, anti-mouse IFN-γ mAb and IL-17 ELISA kits were bought from BioLegend (San Diego, CA, USA), Recombinant human TGFβ1.2 was obtained from eBioscience (San Diego, CA, USA). L-lactate detection kit was obtained from Eton bioscience (San Diego, CA, USA). The LADH inhibitor GNE-140 was purchased from MCE (Minneapolis, MN). Pertussis toxin was obtained from List Biological Lab (Campbell, CA, USA). The glycolysis stress test kit and mitochondria stress test kit were obtained from Agilent Technologies (Palo Alto, Calif.). The myelin oligodendrocyte glycoprotein (MOG) 35-55 was purchased from GL Biochem Corporation (Shanghai, China). Iscove’s Modified Dulbecco Medium (IMDM), Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were ordered from Gibco (South Logan, UT, USA). Non-viable, desiccated Mycobacterium tuberculosis H37 RA and incomplete Freund adjuvant (IFA) was bought from BD Difco (Detroit, MI, USA); L-sodium lactate, Phorbol 12-myristate 13-acetate and Ionomycin were bought from Sigma Aldrich Company (St. Louis, MO).