Murine hearts were harvested and protein lysates were prepared as previously described.[14 (link)] Twenty micrograms of whole heart lysate were resolved by 12% SDS-PAGE or 3–8% Tris-Acetate gel and transferred to a nitrocellulose membrane (GE Healthcare; Whatman, NJ). Antibodies used: Cx43 (3512S, Cell Signaling), phospho-Cx43 (Ser368, 3511, Cell Signaling) Cx40 (AB1726, Millipore), ryanodine receptor (RyR) (34C, Developmental Studies Hybridoma Bank), phospholamban (PLB) (8495, Cell Signaling), phospho-PLB (07–052, Millipore), SERCA2a (2862, Abcam), Amphiphysin 2 (Bin1) (H-100, SC-30099, Santa Cruz), DHPR (MA3-920, Thermo), Junctophilin2 (SC-51313, Santa Cruz), HRP-coupled goat anti-rabbit IgG (Jackson ImmunoResearch), and rabbit anti-gamma-tubulin (T6557, Sigma).
Junctophilin2
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Junctophilin2 is a protein that plays a role in the formation and maintenance of junctional membrane complexes, which are specialized structures that connect the endoplasmic reticulum and the plasma membrane. It functions to facilitate communication and signaling between these two cellular compartments.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by GE Healthcare (1 mentions)
Ab1726, by Merck Group (1 mentions)
Hrp coupled goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Serca2a, by Abcam (1 mentions)
Anti mouse igg hrp linked whole antibody, by GE Healthcare (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using junctophilin2
1
Cardiac Protein Expression Analysis
2
Immunoblotting with Junctophilin-2 and GAPDH
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Primary antibodies for immunoblotting were Junctophilin-2 (1:1000) (Santa Cruz Biotechnology Inc., Texas, USA, sc-51313) and GAPDH (1:1000) (Santa Cruz, sc-20357). Secondary antibodies were anti-rabbit or anti-mouse IgG HRP-linked whole antibody (GE Healthcare, Oslo, Norway).
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