Secondary anti mouse igg hrp antibody

The following fluorescence-labelled antibodies were used: anti-human ICAM-1 (CD54) antibody (R&D Systems, Minneapolis, MN, USA); anti-human VCAM-1 (CD106) antibody (R&D System); anti-mouse IgG1 Cy2 secondary antibody (for ICAM-1) (Acris Antibodies, Herford, Germany) and anti-mouse IgG Cy3 secondary antibody (for VCAM-1) (Jackson ImmunoResearch Laboratories, Baltimore Pike, MD, USA). Antibodies used for western blot were: anti-human ICAM-1 (CD54) antibody (Cell Signaling Technologies, Danvers, MA, USA); anti-human VCAM-1 (CD106) antibody (Santa Cruz Biotechnology, Dallas, Texas, USA); secondary anti-mouse IgG-HRP antibody (R&D Systems) and secondary anti-rabbit IgG-HRP antibody (R&D Systems, Minneapolis USA). Vybrant^TM carboxyfluorescein-diacetate (CFDA) (Invitrogen, Darmstadt, Germany) was used for staining of monocytes in flow chamber experiments.

Monoclonal antibody against human Oct4A and the secondary anti-mouse IgG HRP antibody was obtained from R&D Systems (Minneapolis, Minnesota, USA). Monoclonal and polyclonal antibodies against human Sox2 and Lin28 were obtained from Cell Signalling Technology (Danvers, Massachusetts, USA). The polyclonal antibody against GAPDH was obtained from IMGENIX (San Diego, CA, USA). Secondary anti-rabbit IgG antibody was obtained from Millipore (Billerica, MA, USA). DAPI Nucleic acid stain and Alex Fluor® 488 goat anti-mouse IgG were obtained from Life Technologies (Carlsbad, CA, USA).