Solanesol (all-trans-prenol-9) was extracted from tobacco Nicotiana tabacum cv. Samsun leaves by supercritical fluid extraction (SFE) with carbon dioxide [17 (link)]. Pren-9 (di-trans-poly-cis-prenol-9) was extracted from the wood of Betula verrucosa. Polyprenols were purified on silica gel columns with hexane and diethyl ether using a step-wise gradient. Identity of the obtained prenols was verified by the HPLC-UV and ESI-MS (described below) methods by comparison with the solanesol standard purchased from Sigma-Aldrich and Pren-9 standard from the Collection of Polyprenols (Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland). Purity of the obtained polyprenols assessed by HPLC-UV was approximately 91 and 98 % for solanesol and Pren-9, respectively. β-Citronellol, nerol, geraniol, t,t-farnesol, citral (a mixture of neral and geranial), tert-butyl hydroperoxide (tBuOOH), pyridine, BSTFA (N,O-bis(trimethylsilyl) trifluoroacetamide)/TMCS (trimethylchlorosilane) (99:1, by vol) and m-chloroperbenzoic acid (mCPBA) were purchased from Sigma-Aldrich. Hexane, methanol, chloroform, isopropanol and ethanol of HPLC grade were purchased from J.T. Baker. All other chemicals of analytical reagent grade were obtained from POCh. Milli-Q water from the Millipore Academic (USA) water purification system was used.
M chloroperbenzoic acid mcpba
Manufactured by Merck Group
Sourced in United States
M-chloroperbenzoic acid (mCPBA) is a chemical compound commonly used as an oxidizing agent in organic synthesis. It is a white, crystalline solid that is primarily used as a source of peroxycarboxylic acid, which can be employed in various chemical reactions. The core function of mCPBA is to serve as an effective oxidizing agent in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Pyridine, by Merck Group (1 mentions)
Bstfa, by Merck Group (1 mentions)
Tert butyl hydroperoxide, by Merck Group (1 mentions)
β citronellol, by Merck Group (1 mentions)
2 hydroxyacetophenone, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
4 protocols using m chloroperbenzoic acid mcpba
1
Extraction and Purification of Polyprenols
2
Synthesis of Substituted Benzaldehydes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Benzaldehyde (purity >99%) was purchased from Chempur (Piekary Śląskie, Poland). Methoxy-substituted benzaldehydes (purity 97–98%), 2′-hydroxyacetophenones (purity 97–98%), and m-chloroperbenzoic acid (m-CPBA, ≤77%) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and 6-methoxyflavanone 4f (purity 98%) was from Alfa Aesar (Kandel, Germany). Other chemicals purchased from Chempur were of analytical grade. Silica gel used for column chromatography (Kieselgel 60, 230–400 mesh) was purchased from (Merck, Darmstadt, Germany).
Hexane was purified by distillation before use for column chromatography. m-Chloroperbenzoic acid (m-CPBA) was dried immediately before use over anhydrous magnesium sulphate(VI) in the solution of methylene chloride (20 mL), then the dried solution of m-CPBA was filtered and concentrated under nitrogen.
Hexane was purified by distillation before use for column chromatography. m-Chloroperbenzoic acid (m-CPBA) was dried immediately before use over anhydrous magnesium sulphate(VI) in the solution of methylene chloride (20 mL), then the dried solution of m-CPBA was filtered and concentrated under nitrogen.
3
Synthesis of Phthalocyanine Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents were dried and distilled according to published procedures.38 Hydrogen peroxide (35%) was obtained from Sigma-Aldrich. m-Chloroperbenzoic acid (m-CPBA) purchased from Sigma-Aldrich with 85% peroxide content was recrystallized from pentane prior to use. Phthalocyaninatoiron(ii ) (1 ),39 tetra-(adamantylsulfonyl) phthalocyaninatoiron(ii ) (2 ),40 1,2-dicyano-4,5-bis (hexylthio) benzene (4 ),41 homoleptic complex 8 ,14a (FePc)2N,25 and [FePc(SO2tBu)4]2N,16 (link) were prepared according to literature protocols.
4
Quantitative Analysis of Alkanes and Aromatic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
m-Chloroperbenzoic acid (mCPBA), potassium hydroxide, sodium thiosulfate, sodium sulfate, methanol, toluene, n-hexane, and dichloromethane (the last two were distilled before use) were provided by Sigma-Aldrich (St. Louis, MO, USA). Ethanol was purchased from Supelco (Bellefonte, PA, USA) and milli-Q water was obtained with a Millipore system (Bedford, MA, USA).
A standard mixture of n-C10–40 n-alkanes (added with n-C50) in hexane was used to verify GC performance, which contained even-numbered alkanes in the specified range, each at 0.05 mg/mL. An internal standard solution (IS) was used to verify the transfer windows of the fractions from the LC to the GC and to quantify the MOH levels. The IS contained n-C13 at 0.15 mg/mL, 1,3,5-tri-tert-butylbenzene (TBB), n-C11, cyclohexylcyclohexane (CyCy), n-pentylbenzene (5B), 1-methylnaphthalene (1-MN), 2-methylnaphthalene (2-MN) at 0.30 mg/mL, and 5-α-cholestane (Cho) and perylene (Per) at 0.60 mg/mL in toluene. Both standard solutions were obtained from Restek (Bellefonte, PA, USA), and they were stored at −18 °C.
A standard mixture of n-C10–40 n-alkanes (added with n-C50) in hexane was used to verify GC performance, which contained even-numbered alkanes in the specified range, each at 0.05 mg/mL. An internal standard solution (IS) was used to verify the transfer windows of the fractions from the LC to the GC and to quantify the MOH levels. The IS contained n-C13 at 0.15 mg/mL, 1,3,5-tri-tert-butylbenzene (TBB), n-C11, cyclohexylcyclohexane (CyCy), n-pentylbenzene (5B), 1-methylnaphthalene (1-MN), 2-methylnaphthalene (2-MN) at 0.30 mg/mL, and 5-α-cholestane (Cho) and perylene (Per) at 0.60 mg/mL in toluene. Both standard solutions were obtained from Restek (Bellefonte, PA, USA), and they were stored at −18 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!