T 550

Manufactured by Alomone

Sourced in Israel

The T-550 is a laboratory instrument designed for the measurement and analysis of physical and chemical properties. It is a versatile and reliable tool that can be used in various research and testing applications. The T-550 provides accurate and precise data, making it a valuable asset in a wide range of scientific disciplines.

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Lab products found in correlation

8 protocols using t 550

Neuromodulatory Effects of Atropine, Muscarine, and TTX

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The following drugs were used: atropine (Sigma-Aldrich #A0132), muscarine (Sigma-Aldrich #M6532) and TTX (Alomone Labs #T-550). In all cases stock solutions were prepared and diluted in external solution to the final concentration before experiments. When an immediate response to a drug was examined, a glass pipette filled was placed in close proximity to the AL and was emptied using a pico injector (Harvard Apparatus, PLI-100). When long incubation of 30 minutes was used, the entire bath chamber was filled with external solution containing the drug.

Rozenfeld E., Lerner H, & Parnas M. (2019). Muscarinic Modulation of Antennal Lobe GABAergic Local Neurons Shapes Odor Coding and Behavior. Cell Reports, 29(10), 3253-3265.e4.

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Neuromodulator Application in Olfaction

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The following drugs were used: atropine (Sigma-Aldrich #A0132), nicotine (Sigma-Aldrich #N3876), muscarine (Sigma-Aldrich #M6532), acetylcholine (Sigma-Aldrich #A6625) and TTX (Alomone Labs #T-550). In all cases, stock solutions were prepared were diluted to the final concentration before experiments. Drugs were applied either by bath application or were injected directly to the AL using a pico-injector (Harvard Apparatus, PLI-100).

Rozenfeld E., Tauber M., Ben-Chaim Y, & Parnas M. (2021). GPCR voltage dependence controls neuronal plasticity and behavior. Nature Communications, 12, 7252.

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Dissociated P0 cortical cell culture

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Dissociated P0 cortical cells were cultured on coverslips contained in 35-mm dishes for immunofluorescence or directly in 35-mm dishes for Western blotting. Cultures were exposed to insulin (A11382II, Thermo), D-(+)-glucose (G8270, Sigma), D-mannitol (M4125, Sigma), methylglyoxal (6.49 m stock, M0252, Sigma), or tetrodotoxin (TTX; T-550; Alomone Labs) by dilution of the drug in growth media to 2× final concentration, syringe-filtration through 0.22-μm PES membranes (229746, CELLTREAT Scientific Products), prewarming to 37°C, and then adding 1 ml to 35-mm dishes containing 1 ml of conditioned medium for a 1× final concentration.

Griggs R.B., Nguyen D.V., Yermakov L.M., Jaber J.M., Shelby J.N., Steinbrunner J.K., Miller J.A., Gonzalez-Islas C., Wenner P, & Susuki K. (2021). The Type 2 Diabetes Factor Methylglyoxal Mediates Axon Initial Segment Shortening and Alters Neuronal Function at the Cellular and Network Levels. eNeuro, 8(5), ENEURO.0201-21.2021.

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Optogenetic Activation of Thalamocortical Fibers

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For activation of thalamocortical fibers, an extracellular stimulating electrode (concentric bipolar Pt/Ir electrode, 33 G; FHC) was placed in the external capsule in close proximity to visually identified mCherry-expressing fiber bundles. Fibers were activated by short, bipolar electrical pulses (400 μs, <100 μA). Opsins were activated using a 590 nm light emitting diode (LED; M590L2-C2; Thorlabs) delivered through the microscope illumination path which included a custom dichroic in order to reflect the 590 nm activation wavelength while collecting green fluorescence emission. Light power densities were calculated by measuring the light transmitted through the objective using a power meter (Thorlabs PM100A with S146C sensor) and dividing by the illumination area, calculated from the microscope objective field number and magnification25 (link). TTX (1 μM; T-550, Alomone), D-AP5 (25 μM; ab120003; Abcam) and CNQX (10 μM; C-141, Alomone) were bath applied where indicated.

Mahn M., Prigge M., Ron S., Levy R, & Yizhar O. (2016). Biophysical constraints of optogenetic inhibition at presynaptic terminals. Nature neuroscience, 19(4), 554-556.

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Neuromodulator Delivery to Mushroom Body

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The following drugs were used: Dopamine Hydrochloride (Alfa Aesar #A11136), ACh (Sigma-Aldrich #A6625) and TTX (Alomone Labs #T-550). In all cases stock solutions were prepared and diluted in external solution to the final concentration before experiments. For local application, a glass pipette filled was placed in close proximity to the MB lobe and was emptied using a pico injector (Harvard Apparatus, PLI-100).

Manoim J.E., Davidson A.M., Weiss S., Hige T, & Parnas M. (2022). Lateral Axonal Modulation is Required for Stimulus-Specific Olfactory Conditioning in Drosophila. Current biology : CB, 32(20), 4438-4450.e5.

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Nicotine and TTX Application Protocols

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The following drugs were used: nicotine (Sigma-Aldrich #N3876) and TTX (Alomone Labs #T-550). In all cases, stock solutions were prepared were diluted to the final concentration before experiments. Drugs were applied either by bath application or were injected directly to the AL using a pico-injector (Harvard Apparatus, PLI-100).

Rozenfeld E., Ehmann N., Manoim J.E., Kittel R.J, & Parnas M. (2023). Homeostatic synaptic plasticity rescues neural coding reliability. Nature Communications, 14, 2993.

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Brain Slices Pharmacological Modulation

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Brain slices were incubated with PGE₂ and EP2 agonist for at least 35 min, while EP receptor antagonists and COX inhibitors were applied for at least 50 min prior to recording.
PGE₂ (Cat# 2296), PF 04418948 (EP2 receptor antagonist; Cat# 4818), L-798,106 (EP3R antagonist; Cat# 11129), BGC 20-1531 (EP4R antagonist; Cat# 5327), butaprost (EP2 receptor agonist; Cat# 13740), SC-560 (COX-1 inhibitor; Cat# 1550), and SC-236 (COX-2 inhibitor; Cat# 3919) were from Tocris Bioscience. ONO-8711 (EP1 receptor antagonist; Cat# 14070) was from Cayman Chemicals. Acetaminophen (COX inhibitor; A7085) and H89 (PKA inhibitor; B1427) were from Sigma-Aldrich.
The following drugs were perfused through the recording chamber: Ouabain octahydrate (Na⁺/K⁺-ATPase inhibitor; Cat# 03125) and picrotoxin (Cl⁻ channel blocker; P1675) from Sigma-Aldrich; Tetrodotoxin (Na⁺ channel blocker; T-550) from Alomone Labs (Jerusalem, Israel); D-AP5 (NMDA receptor antagonist; ab120003) from Abcam; and DNQX (non-NMDA receptor antagonist; Cat# 0189) from Tocris Bioscience.

Fang L.Z., Linehan V., Licursi M., Alberto C.O., Power J.L., Parsons M.P, & Hirasawa M. (2023). Prostaglandin E2 activates melanin-concentrating hormone neurons to drive diet-induced obesity. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2302809120.

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Optogenetic Activation of Thalamocortical Fibers

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Mahn M., Prigge M., Ron S., Levy R, & Yizhar O. (2016). Biophysical constraints of optogenetic inhibition at presynaptic terminals. Nature neuroscience, 19(4), 554-556.

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