Volatile extracts were analyzed on a GC (Agilent Technologies, 6890 N, Stockport, UK), equipped with an FID and a HP-1 capillary column (50 m × 0.32 mm i.d., 0.52 μm film thickness). The oven temperature was maintained at 30 °C for 1 min and programmed at 5 °C/min to 150 °C, where it was held for 0.1 min, then at 10 °C /min to 230 °C and held for 27 min. The carrier gas was hydrogen. One μL of sample was injected into the injection port of the equipment manually. GC-MS analysis of eluted volatiles was performed using a Waters Autospec Ultima mass spectrometer couple to an Agilent 6890 GC fitted with a HP-1 capillary column (50 m × 0.32 mm id, 0.52 μm film thickness). Ionization was by electron impact (70 eV, source temperature 220 °C). Helium was the carrier gas. The oven temperature was maintained at 30 °C for 5 min, and then programmed at 5 °C/min to 250 °C. Tentative identifications were made by comparison of mass spectra with NIST 2005 mass spectral database. Confirmation of peak identity was made by comparison of their Kováts index (KI) values and GC peak enhancement with authentic compounds. Compounds were quantified using the single point external method with an n-alkane (C7-C22) mixture. Statistical analysis of cowpea volatile data (ng/h) was done using two-sample t-test on log-transformed values (GenStat version 16).
Hp 1 capillary column
Manufactured by Agilent Technologies
Sourced in United States
The HP-1 capillary column is a versatile gas chromatography (GC) column designed for a wide range of applications. It features a non-polar stationary phase that enables the separation of a variety of analytes, including hydrocarbons, alcohols, and other organic compounds. The column is characterized by its high thermal stability and inertness, ensuring reliable and consistent performance in GC analysis.
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Lab products found in correlation
5975c vl msd, by Agilent Technologies (2 mentions)
Gc 14c, by Shimadzu (1 mentions)
Autosystem xl, by PerkinElmer (1 mentions)
Spectrum one, by PerkinElmer (1 mentions)
Mass selective detector msd 5973, by Agilent Technologies (1 mentions)
Agilent 5975n, by Agilent Technologies (1 mentions)
Agilent 6890n, by Agilent Technologies (1 mentions)
Autospec ultima mass spectrometer, by Agilent Technologies (1 mentions)
6850 gas chromatograph, by Agilent Technologies (1 mentions)
Agilent 6890 series gc system, by Agilent Technologies (1 mentions)
12 protocols using hp 1 capillary column
1
Volatile Profiling of Cowpea using GC-FID and GC-MS
2
Quantification of MTBE and TBA
3
GC Analysis of Volatile Organic Compounds
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Entrained VOCs were analyzed using a Hewlett-Packard 7890 GC machine (Agilent Technologies) equipped with a cool-on column injector, a non-polar HP-1 capillary column (50 m, 0.32 mm internal diam, 0.52 μm film thickness) and a flame ionization detector (FID). Four μl of headspace sample were injected into the injector port of the GC instrument. The oven temperature was maintained at 30 °C for 2 min and then programmed at 5 °C min-1 to 250 °C. The carrier gas was hydrogen. Data were analyzed using HP Chemstation software. Quantification was done by dividing peak areas by known amounts of external standards. The emission rate in terms of ng plant−1 h−1 was obtained by multiplying inverse of the proportion of the total headspace used and dividing by the number of hours in the sampling period. Four replicate headspace samples were analyzed for each treatment.
4
Quantitative Gas Chromatography of Hydrocarbons
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The standard C24D50 was added to 10 mg of the saturated hydrocarbon samples to conduct the gas chromatography measurements with Shimadzu GC-14C (Shimadzu, Japan). The analytical conditions were as follows: Agilent HP-1 capillary column (30 m × 0.32 mm × 0.25 μm) with carrier gas (N2) flow rate of 2 mL/min, air flow rate of 300 mL/min, H2 flow rate of 30 mL/min, makeup gas flow rate of 28 mL/min, and injection volume of 1 μL. The temperature program was 1-min isothermal heating at 70 °C, and the oven temperature was increased to 100 °C at a rate of 8 °C/min (held for 2 min) and then increased to 300 °C at a rate of 4 °C/min and held for 20 min. The inlet temperature and the detector temperature were both 300 °C.
5
Carbohydrate structural analysis protocol
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Native and permethylated polysaccharides were hydrolyzed with 2 M trifluoroacetic acid (TFA) at 125 °C for 1 h. Alditol acetates were prepared as previously described [15 (link)]. Permethylation of the Epol H111-INS was achieved following the protocol by Harris [16 (link)].
Analytical GLC was performed on a Perkin-Elmer Autosystem XL gas chromatograph equipped with a flame ionization detector and using He as carrier gas. An HP-1 capillary column (Agilent Technologies, 30 m) was used to separate alditol acetates (temperature program: 3 min at 150 °C, 150–270 °C at 3 °C/min, 2 min at 270 °C), PMAA (temperature program: 1 min at 125 °C, 125–240 °C at 4 °C/min, 2 min at 240 °C), and trimethylsilyl (TMS) (+)-2-butyl glycosides, for the determination of the absolute configuration of the sugar residues [17 (link)], (temperature program: 1 min at 50 °C, 50–130 °C at 45 °C/min, 1 min at 130 °C, 130–200 °C at 1 °C/min, 10 min at 200 °C). GLC–MS analyses were carried out on an Agilent Technologies 7890A gas chromatograph coupled to an Agilent Technologies 5975C VL MSD, using the same temperature programs reported above.
Analytical GLC was performed on a Perkin-Elmer Autosystem XL gas chromatograph equipped with a flame ionization detector and using He as carrier gas. An HP-1 capillary column (Agilent Technologies, 30 m) was used to separate alditol acetates (temperature program: 3 min at 150 °C, 150–270 °C at 3 °C/min, 2 min at 270 °C), PMAA (temperature program: 1 min at 125 °C, 125–240 °C at 4 °C/min, 2 min at 240 °C), and trimethylsilyl (TMS) (+)-2-butyl glycosides, for the determination of the absolute configuration of the sugar residues [17 (link)], (temperature program: 1 min at 50 °C, 50–130 °C at 45 °C/min, 1 min at 130 °C, 130–200 °C at 1 °C/min, 10 min at 200 °C). GLC–MS analyses were carried out on an Agilent Technologies 7890A gas chromatograph coupled to an Agilent Technologies 5975C VL MSD, using the same temperature programs reported above.
6
Comprehensive Characterization of Collagen
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GLC analyzes were performed using an Agilent Technologies 6850 gas chromatograph equipped with an FID detector and Agilent HP-1 capillary column (30 m × 0.32 mm × 0.25 µm). Analysis conditions: T 50 °C for 1 min., rate: 20 °C/min., T 230 °C for 15 min. 1H-NMR spectra were recorded on a Bruker Avance AC 300 spectrometer (Billerica, MA, USA) operating at 300.21 and 75.44 MHz, respectively. The chemical shift values of the spectra are reported in δ units with reference to the residual solvent signal. IR spectra were collected in the range 4000–400 cm−1 using a PerkinElmer Spectrum One spectrophotometer. Differential scanning calorimetry (DSC) of collagen samples was performed on a Netzsch STA 409 cell (Selb, Germany) fitted with an air-cooling compressor at ambient temperature and a controller Netzsch TASC 414/3. The instrument temperature was calibrated using indium as the standard. Collagen samples (about 7.0 mg) were weighed into an aluminum oxidized melting pot and sealed. Samples were heated from 30 °C to 120 °C at a scanning rate of 10 °C/min. A sealed melting pot filled with Al2O3 (about 7.0 mg) was used as the reference.
7
GC-MS Analysis of Organic Compounds
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GC-MS analysis was performed on an Agilent 19091S apparatus, fitted with a fused silica HP-1 capillary column (30×0.25 cm id; 0.50 µm film thickness), and coupled to an Agilent Mass Selective Detector MSD 5973. The temperature program was increased to 100°C for 0.5 min, then increased to 280°C at a rate of 4°C/min, and maintained for 20 min. The other parameters were as follows: injection temperature, 280°C; EI, 70Ev; Carrier gas, He at 1 ml/min; injection volumn, 1 µl; split ratio, 1∶60; and mass range, m/z 40−600.
8
GC-MS Analysis of A. scoparia Essential Oil
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The constituents of the A. scoparia essential oil was confirmed by gas chromatography using a GC system (Agilent 6890N, Agilent Technologies Incorporated, California, United States), which was equipped with an HP-1 capillary column (30 m × 0.25 mm × 0.25 μm film thickness). The oven temperature was programmed at 60 °C for 3 min, with an increase of 10 °C/min, until 280 °C for 5 min. The carrier gas was helium at a flow rate of 1.0 mL/min, the split ratio was 50:1, and the injection volume was 1.0 μL.
The mass spectrometer (Agilent 5975N, Agilent Technologies Incorporated, Palo Alto, CA, USA) used an electron ionization source with 70 eV ionization energy. The ion source temperature was 230 °C, with a scanning range between 20 and 650 m/z. The temperature of the quadrupole was 150 °C, and the mass spectrum acquisition delay time was 2 min. The constituents were identified based on their retention index and the use of the mass spectral libraries (National Institute of Standards and Technology, NIST databases). The area normalization method was used to calculate the relative content of each constituent.
The mass spectrometer (Agilent 5975N, Agilent Technologies Incorporated, Palo Alto, CA, USA) used an electron ionization source with 70 eV ionization energy. The ion source temperature was 230 °C, with a scanning range between 20 and 650 m/z. The temperature of the quadrupole was 150 °C, and the mass spectrum acquisition delay time was 2 min. The constituents were identified based on their retention index and the use of the mass spectral libraries (National Institute of Standards and Technology, NIST databases). The area normalization method was used to calculate the relative content of each constituent.
9
GC-MS Analysis of Volatile Compounds
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It was carried out on Agilent technologies 7890A gas chromatograph coupled to an Agilent Technologies 5975C VL MSD equipped with an HP-1 capillary column (Agilent Technologies, 30 m). The temperature program used was as follows: at 70 °C for 2 min, 70–230 °C at 20 °C/ min. The MS data (total ion chromatogram TIC) were acquired in the full scan mode (m/z of 50–500) at a scan rate of 1000 amu using the electron impact ionization (e.i.) with an electron energy of 70 eV. The acquired spectrum was searched against the standard NIST-05 library/ WILEY 7 library.6 [56 (link), 57 (link)].
10
Coupled GC-MS Analysis of Volatile Compounds
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Coupled GC-mass spectrometry (GC-MS) analysis of eluted VOCs was performed using an Agilent GC-Mass Selective Detector System (5977B inert plus, source temperature 220 °C) coupled with an Agilent GC (8890 GC) fitted with a HP-1 capillary column (50 m × 0.32 mm inner diameter, 0.52 μm film thickness). Injection of eluted VOC samples was via a cool-on-column injector, with helium as the carrier gas. The oven temperature was maintained at 30 °C for 1 min and increased at 5 °C/min to 150 °C, where it was held for 0.1 min, then at 10 °C/min to 230 °C and held for 26 min. Tentative identifications were made by comparison of mass spectra with NIST11 mass spectral database and by comparison of GC retention indices (Kováts Index, KI). Where commercial standards were available, tentative identifications by GC-MS was confirmed by peak enhancement on a HP-1 column by co-injection with authentic compounds, using an Agilent 7890 A GC equipped with a cool on-column injector, FID and a 50 m × 0.32 mm i.d. HP-1 column (0.52 μm film thickness).
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