Infigratinib

3T3 cells expressing each FGFR variant were cultured in DMEM-F12 medium with 1.5% FBS for 2 weeks. The remaining 3T3 cells were mixed and treated with the indicated concentrations (0.1 nM–10 µM) of inhibitors for 5 days. The inhibitors were one multikinase FGFR inhibitor (dovitinib), five FGFR1/2/3 inhibitors (AZD4547, infigratinib, E7090, futibatinib, and pemigatinib), one pan-FGFR inhibitor (erdafitinib), and one FGFR4 inhibitor (H3B-6527). The experiment was conducted in triplicate. We calculated the number of each bar code using the MANO method. Considering the different doubling times of the transduced cells, dimethyl sulfoxide (DMSO)-treated cell mixtures were used as the reference control for scaling the bar code count of each clone. The relative growth inhibition of each cell clone was calculated as the ratio of the average read number across triplicates to that of the DMSO control. All inhibitors used in the assay, except E7090 (provided from Eisai Co., Ltd., Tokyo, Japan), were commercially purchased: dovitinib, AZD4547, infigratinib, pemigatinib (all from MedChem Express, Monmouth Junction, NJ, USA), futibatinib (Cayman Chemical, Ann Arbor, MI, USA), and erdafitinib and H3B-6527 (both from Selleckchem, Houston, TX, USA).