Xanthohumol (Xn), purity >98%, was provided by the Chengdu Herbpurify CO., LTD (Chengdu, China). LPS (Escherichia coli 055:B5) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C and Brusatol (specific inhibitors of the AMPK, and Nrf2, respectively), and tert-butyl hydroperoxide (t-BHP) were offered by Sigma-Aldrich (St. Louis, MO). Penicillin and streptomycin, Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were acquired from Invitrogen-Gibco (Grand Island, NY). Antibodies against Nrf2, Keap1, GCLC, HO-1, NQO1, GCLM, Trx-1, Txnip, NLRP3, casapase-1, ASC, IL-1β, Lamin B and β-actin were supplied by Cell Signaling (Boston, MA, USA) or Abcam (Cambridge, MA, USA). The Annexin V fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) apoptosis kit were offered from Beyotime Institute of Biotechnology (Jinlin, China). In addition, GSH, MDA, SOD and MPO test kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals were offered by Sigma-Aldrich (St. Louis, MO, USA), if not otherwise indicated.
Casapase 1
Manufactured by Cell Signaling Technology
Sourced in United States
Caspase-1 is an enzyme that plays a central role in the inflammatory response. It is responsible for the proteolytic activation of the proinflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18). Caspase-1 is a key mediator of the inflammatory response and is involved in the regulation of cell death and immune function.
Automatically generated - may contain errors
Lab products found in correlation
Lps escherichia coli 055 b5, by Merck Group (2 mentions)
Il 1β, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Glutathione (gsh), by Nanjing Jiancheng (2 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Lamin b, by Cell Signaling Technology (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc propidium iodide apoptosis kit, by Beyotime (1 mentions)
Mpo test kit, by Nanjing Jiancheng (1 mentions)
Txnip, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tert butyl hydroperoxide tbhp, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Antibodies against, by Abcam (1 mentions)
Atg12, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Atg16, by Cell Signaling Technology (1 mentions)
Sod test kit, by Nanjing Jiancheng (1 mentions)
P iκbα iκbα, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
2 protocols using casapase 1
1
Xanthohumol Protects Against Oxidative Stress
2
Licochalcone A Modulates Autophagy and Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Licochalcone A (Lico A), purity up to 98%, was provided by the Chengdu Herbpurify CO., LTD (Chengdu, China). Dimethyl sulfoxide (DMSO), d-Galactosamine, LPS (Escherichia coli 055:B5), and inhibitors of autophagy (3-methyladenine, 3-MA) were offered by Sigma-Aldrich (St. Louis, MO, USA). Antibodies against P-AMPK/AMPK, Nrf2, HO-1, and Lamin B were offered by Abcam (Cambridge, MA, USA); and Atg7, Atg16, Beclin1, Atg12, Atg5, Atg3, LC3, NLRP3, ASC, Casapase-1, IL-1β, P-ERK/ERK, P-P38/P38, P-P65/P65, P-IκBα/IκBα, P-JNK/JNK, TFEB, P-P62, and β-actin were obtained from Cell Signaling. Additionally, ALT, AST, MDA, ROS, GSH, and SOD test kits were obtain Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), if not otherwise indicated.
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