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2 protocols using tno155

1

Murine Tumor Model Drug Preparation

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TNO155 (#HY-136173) and SHP099 (#HY-100388A) were purchased from MedChemExpress, and ceritinib (#S7083), lorlatinib (#S7536), and crizotinib (#S1068) were purchased from Selleck Chemicals. Inhibitors were resuspended in DMSO. For murine experiments, lorlatinib (#HY-12215) and TNO155 (#HY-136173) were purchased from MedChemExpress or TNO155 was provided by Novartis AG. Drugs were resuspended in final concentrations of 0.5% methylcellulose + 0.5% Tween 80 in water.
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2

NSCLC Cell Line Authentication and Compound Screening

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The NSCLC NCI-H23 and NCI-H2122 cell line were purchased from the ATCC. The authenticity of the cells was confirmed by short tandem repeat profiling on September 9, 2020. All cell lines were authenticated and verified as mycoplasma free every month. The cells were maintained in a humidified atmosphere of 5% CO 2 at 37 C in RPMI1640 medium (Sigma-Aldrich Corp.) supplemented with 10% FBS and 1% penicillin-streptomycin. Sotorasib, adagrasib, BI-3406, and TNO155 were obtained from MedChemExpress (Monmouth Junction); crizotinib was purchased from Chem Scene (Monmouth Junction); capmatinib was purchased from Selleck Chemicals; and selumetinib was bought from ShangHai Biochempartner. Compounds were dissolved in dimethyl sulfoxide to a final concentration of 10 mmol/L and stored at À20 C until use.
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