Dach1

Manufactured by ABclonal

DACH1 is a laboratory equipment product designed for scientific research applications. It serves as a tool for sample preparation, separation, and analysis. The core function of DACH1 is to facilitate the handling and processing of various biological or chemical samples, but a detailed description of its intended use is not available.

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Lab products found in correlation

N cadherin, by Proteintech (2 mentions) E cadherin, by Immunoway (2 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions) Ripa buffer, by Solarbio (1 mentions) P gsk 3β, by ABclonal (1 mentions) Gsk3β, by ABclonal (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)

2 protocols using dach1

Western Blot Analysis of Cell Signaling

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Cell lysates were prepared in RIPA buffer (#R0020; Solarbio) with protease inhibitors (1 mM PMSF, #P0100; Solarbio), separated with SDS‐PAGE and transferred onto PVDF membranes, which were blocked in 5% milk, and then incubated with the primary antibody overnight at 4°C followed with secondary antibody (mouse IgG, #5220‐0341; Seracare; rabbit IgG, #5220‐0336; Seracare) for 2 h. The primary antibodies were used as follows: β‐Catenin (#8480s; CST), E‐cadherin (#YM0207; IMMUNOWAY), N‐cadherin (#22018; PROTEINTECH), DACH1 (#A3823; ABclonal), CEBPA (#8178S; CST), p65 (#8242; CST), p‐p65 (#3033; CST), AKT (#4691; CST), p‐AKT (#4060; CST), LATS2 (#5888; CST), ERK1/2 (#4695; CST), p‐ERK1/2 (#4370; CST), p38 (#8690; CST), p‐p38 (#4511; CST), BRAF (#14814; CST), c‐Jun (#9165; CST), JunB (#3753; CST), c‐FOS (#2250; CST), TPO (#ab203057; Abcam), NIS (#ab242007; Abcam), PAX8 (#ab97477; Abcam), GSK3β (#A2081; ABclonal), p‐GSK3β (#AP0039; ABclonal).

Zhang P., Guan L., Sun W., Zhang Y., Du Y., Yuan S., Cao X., Yu Z., Jia Q., Zheng X., Meng Z., Li X, & Zhao L. (2024). Targeting miR‐31 represses tumourigenesis and dedifferentiation of BRAFV600E‐associated thyroid carcinoma. Clinical and Translational Medicine, 14(5), e1694.

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Comprehensive Immunohistochemical and Immunofluorescence Analysis of Tumor Tissues

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Tumour tissues were fixed in 4% PFA, embedded in paraffin and sectioned, then 5‐µm sections were stained with haematoxylin and oeosin (H&E) for morphology analysis. Immunohistochemistry (IHC) and immunofluorescence (IF) staining were performed following a standard procedure.
²⁸ The primary antibodies include: Ki‐67 (#ab16667; Abcam), β‐Catenin (#8480s; CST), E‐cadherin (#YM0207; IMMUNOWAY), N‐cadherin (#22018; PROTEIN TECH), DACH1 (#A3823; ABclonal), CEBPA (#8178S; CST), PAX8 (#ab97477; Abcam), cyclin D1 (#PTM‐6029; PTM BIO). To evaluate the staining intensity in IHC, the immunoreactive score (IRS Score) was calculated as previous study.
²⁷ For IF staining, tissues were pretreated by 0.5% Triton X‐100, blocked with 5 mg/mL BSA and then incubated with primary antibody anti‐Thyroglobulin (#ab156008; Abcam) at 4°C overnight, followed with Goat anti‐Rabbit IgG (H+L) Cross‐Adsorbed Secondary Antibody, Alexa Fluor 594 (# A‐11012; Invitrogen) at room temperature for 2 h.

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