HepG2 were lysed using RIPA buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% NP40, 0.5% Sodium Deoxycholate, 0.1% SDS, Halt™ Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific)). Protein extracts (30 μg for NDUFS8, 50 μg for TIAM2 and 5 μg for all other proteins) were immunoblotted using the indicated primary antibodies (Supplementary Table S2 ) and anti-mouse, anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
Anti rat igg linked hrp secondary antibodies
Manufactured by GE Healthcare
Anti-rat IgG-linked HRP secondary antibodies are laboratory reagents used to detect and quantify rat immunoglobulin G (IgG) in various immunoassays. The antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric reaction, allowing for the visualization and measurement of target rat IgG.
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Lab products found in correlation
2 protocols using anti rat igg linked hrp secondary antibodies
1
Western Blot Analysis of HepG2 Cells
2
HEK293T Cell Lysis and Immunoblotting
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HEK293T and pOZ-NF90-FH HEK293T were lysed using RIPA buffer (50 mM Tris-HCl pH = 7.5, 150 mM NaCl, 1 % NP40, 0.5 % Sodium Deoxycholate, 0.1 % SDS, Halt™ Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific)), unless otherwise indicated. Protein extracts were immunoblotted using the indicated primary antibodies (Additional file 8 : Table S2) and anti-mouse, anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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