For tamoxifen treatment, mice were injected intraperitoneally with tamoxifen (1 mg per mouse) in corn oil daily for four consecutive days and analyzed 8 days after the last injection. For experiments involving an immune challenge, chimeras were given tamoxifen (1 mg per mouse) in corn oil daily for four consecutive days via oral gavage and challenged with antigens or influenza at day 8 after the last tamoxifen administration. For NP-OVA immunization experiments, antigen for immunization was prepared by mixing NP20-OVA (20 molecules of NP linked to OVA; Biosearch Technologies), 10% KAL(SO4)2 dissolved in PBS at a ratio of 1:1, in the presence of LPS (Escherichia coli strain 055:B5; Sigma) at pH 7. Mice were immunized intraperitoneally (100 μg NP-OVA and 10 μg LPS) for analysis of NP-specific antibody response. Nine days after immunization, sera, spleens and mesenteric lymph nodes were collected from the mice. Thirteen days after infection, spleens, mediastinal lymph nodes and lungs from the mice were harvested for analysis. For T-independent immune response, mice were given 50 μg TNP-LPS (Biosearch Technologies) intraperitoneally. Sera and spleens were collected on day 7 after immunization.
Np20 ova
Manufactured by Biosearch Technologies
The NP20-OVA is a laboratory instrument produced by Biosearch Technologies. It is designed for the detection and analysis of ovarian cancer biomarkers. The core function of the NP20-OVA is to perform quantitative measurements of specific proteins or other biomolecules in biological samples.
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2 protocols using np20 ova
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Tamoxifen-Induced Immune Modulation Experiments
2
Tamoxifen-Induced Immune Modulation Experiments
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For tamoxifen treatment, mice were injected intraperitoneally with tamoxifen (1 mg per mouse) in corn oil daily for four consecutive days and analyzed 8 days after the last injection. For experiments involving an immune challenge, chimeras were given tamoxifen (1 mg per mouse) in corn oil daily for four consecutive days via oral gavage and challenged with antigens or influenza at day 8 after the last tamoxifen administration. For NP-OVA immunization experiments, antigen for immunization was prepared by mixing NP20-OVA (20 molecules of NP linked to OVA; Biosearch Technologies), 10% KAL(SO4)2 dissolved in PBS at a ratio of 1:1, in the presence of LPS (Escherichia coli strain 055:B5; Sigma) at pH 7. Mice were immunized intraperitoneally (100 μg NP-OVA and 10 μg LPS) for analysis of NP-specific antibody response. Nine days after immunization, sera, spleens and mesenteric lymph nodes were collected from the mice. Thirteen days after infection, spleens, mediastinal lymph nodes and lungs from the mice were harvested for analysis. For T-independent immune response, mice were given 50 μg TNP-LPS (Biosearch Technologies) intraperitoneally. Sera and spleens were collected on day 7 after immunization.
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