Actcasp 3

Custom-modified miR-148b (5′–6-Cy5–2′Ome–[UCA GUG CAU CAC AGA ACU UUG U]–3′) and custom-modified noncoding control miRNA oligo mimics (5′–[CGG UAC GAU CGC GGC GGG AUA]–3′) were acquired through Integrated DNA Technologies, Inc. (Coralville, Iowa). Primary antibodies for the active and inactive forms of the caspase-3 protein, actCASP-3, and proCASP-3 were purchased from ABCAM (Cambridge, CB2 0AX, U.K.) and Cell Signaling Technology (Danvers, Massachusetts), respectively. A549 cells (Human Caucasian Male Lung Adenocarcinoma), Proteinase K, and sodium acetate buffer solution (pH 5.2) were purchased from Millipore Sigma (St. Louis, MO). Cell culture reagents including Dulbecco’s modified Eagle’s medium (DMEM) for mammalian cell culture, OptiMEM media, fetal bovine serum (FBS), penicillin–streptomycin (PS), and trypsin ethylenediamine tetraacetic acid (EDTA), as well as the NucBlue staining kit, the Verso cDNA Synthesis kit, and the PowerUp SYBR Green Master Mix, were all purchased from Thermo Fisher Scientific (Waltham, Massachusetts). The LIVE/DEAD Viability/Cytotoxicity kit, the Quant-iT PicoGreen dsDNA fluorescence assay kit, and the Trizol RNA isolation kit were purchased from Invitrogen (Carlsbad, California).