Mouse anti human e cadherin antibody

Mouse anti-human E-Cadherin antibody (for western blot) was purchased from BD Bioscience (San Jose, CA, USA). Rabbit anti-human ZO-1, Mouse anti-human α-SMA, FITC-conjugated anti mouse, and FITC-conjugated anti Rabbit antibodies were obtained from Invitrogen (Carlsbad, CA, USA). Rabbit anti-human ZEB1 antibody, Mouse anti-human fibronectin antibody was from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-human SLUG antibody, Rabbit anti-human E-Cadherin antibody (for immunofluorescence) and rabbit anti-human β-actin antibody was purchased from Abcam (Cambridge, MA, USA). Rabbit anti-human Snail antibody was from Santa-Cruze Biotechnology (Santa Cruz, CA, USA). Mouse anti-human Smad2/3, p-Smad2/3, p38, and p-p38 antibodies were from Cell Signaling Technology (Danvers, MA, USA). TRIzol reagent and DAPI (4’,6-diamidino-2-phenylindole) were bought from Invitrogen (Carlsbad, CA, USA). SuperReal PreMix Plus (SYBR Green) reagent kit was from Takara Clontech (Kyoto, Japan). Most of other reagents such as salt and buffer components were analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA).

L-glutamine, penicillin/streptomycin, donkey anti-mouse IgG-Alexa Fluor 488, Chicken anti-rabbit IgG-Alexa Fluor 594, Laemmli sample buffer and Trizol Reagent were purchased from Invitrogen (Carlsbad, CA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 and bovine serum albumin (BSA) were from Sigma (St Louis, MO), and fetal bovine serum (FBS) from Thermo scientific. Rabbit anti α-SMA, anti-CD16 and anti-CD14 bound micromagnetic beads were purchased from Miltenyi Biotec (Auburn, CA), mouse anti-human E-cadherin antibody from BD Biosciences (Mississauga, ON, Canada), and anti-TGF-β₁ monoclonal antibody (mAb) (1D11) from R&D Systems (Minneapolis, MN). Sepasol-RNA I super G (Nacalai tesque), anti-mouse antibodies against CD11b (integrin α_M), CD49d (integrin α₄), CD29 (integrin β₁), CD18 (integrin β₁), CD54 (ICAM-1), and CD106 (VCAM-1) were from BioLegend.

Protein extraction from AEII at 21 days, and Western blotting was carried out as described previously (Cabrera-Benitez et al. 2012 (link)). The following antibodies were used: mouse anti-human E-cadherin antibody (BD Bioscience, 1:2000, San Jose, CA), goat anti-human pro-SP-B polyclonal antibody (Santa Cruz Biotechnology, 1:500), mouse anti-human vimentin (1:1000, BD), rabbit anti-human GAPDH (1:10000, BD), goat anti-mouse, donkey anti-goat, goat anti-rabbit-conjugated to horseradish peroxidase (1:5000, EarthOx).