T98g human glioblastoma

Lactoferrin and lactoferricin, generation 3-diaminobutyric polypropylenimine dendrimer (DAB) and the other chemicals were purchased from Sigma Aldrich (Poole, UK). The expression plasmids encoding Tumor necrosis factor (TNF) α (pORF9-mTNFα) and β-galactosidase (pCMVsport β-galactosidase) were obtained respectively from InvivoGen (San Diego, CA) and Invitrogen (Paisley, UK) and were purified using an Endotoxin-free Giga Plasmid Kit (Qiagen, Hilden, Germany). Passive lysis buffer was from Promega (Southampton, UK). Quanti-iT™ PicoGreen^® dsDNA reagent and tissue culture media were obtained from Invitrogen (Paisley, UK). Bioware^® B16-F10-luc-G5 mouse melanoma was obtained from Caliper Life Sciences (Hopkinton, MA). A431 human epidermoid carcinoma and T98G human glioblastoma were purchased from the European Collection of Cell Cultures (Salisbury, UK).

Generations 3-, 4- and 5- diaminobutyric polypropylenimine (DAB) dendrimers were purchased from Symo-Chem (Eindhoven, The Netherlands). Methoxy PEG succinimidyl carboxymethyl esters of various molecular weights (2 kDa, 5 kDa and 10 kDa) came from Jenkem Technology (Plano, TX, USA). The expression plasmid encoding β-galactosidase (pCMVsport β-galactosidase) was obtained from Invitrogen (Paisley, UK). It was purified using an Endotoxin-free Giga Plasmid Kit (Qiagen, Hilden, Germany). Quanti-iT PicoGreen dsDNA reagent and tissue culture media were purchased from Life Technologies (Paisley, UK). Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) was obtained from Vector Laboratories (Peterborough, UK). Label IT Fluorescein Nucleic Acid Labelling Kit was from Cambridge Biosciences (Cambridge, UK). Passive lysis buffer was obtained from Promega (Southampton, UK). All other materials were purchased from Sigma Aldrich (Poole, UK). Bioware PC-3M-luc-C6 human prostate adenocarcinoma and Bioware B16-F10-luc-G5 mouse melanoma were obtained from Caliper Life Sciences (Hopkinton, MA, USA), while A431 human epidermoid carcinoma, T98G human glioblastoma and DU145 human prostate carcinoma were purchased from the European Collection of Cell Cultures (Salisbury, UK).