G1322a pump

HPLC analyses were performed on an Agilent 1200 HPLC system equipped with a G1322A pump and in-line diode array UV detector using an Agilent Zorbax 300SB-C18 RP column with CH₃CN (0.1% of (TFA)) and water (0.1% of TFA) as the eluent. HPLC traces of Tyr (0.1 mg mL⁻¹), Tyr (1 mg mL⁻¹, 200 µL) treated with CuS-I solution (1 mg mL⁻¹, 800 µL) and H₂O₂ (100 μM, 1 mL) for 8 h at 37 °C were detected.

CBT was purchased from Shanghai Chemical Pharm-Intermediate Tech. Co.. Electrospray ionization-mass spectrometry (ESI-MS) spectra were obtained on a Q Exactive Plus (ThermoFisher Corporation). ¹H NMR and ¹³C NMR spectra of NIR-CBT were recorded on a Bruker AV 400 MHz spectrometer. High resolution electrospray ionization-mass spectrometry (HR-ESI-MS) spectra of B, C, D, and NIR-CBT were recorded on a Finnigan LCQ Advantage ion trap mass spectrometer (ThermoFisher Corporation) which was equipped with a standard ESI source. Dynamic light scattering (DLS) spectrum of NIR-CBT-NP was obtained on a NanoBrook 90PLUS PALS particle size analyzer. The spectrum of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) was obtained on an Utralextrem III (Bruker Daltonics). An Agilent 1200 HPLC system equipped with a G1322A pump and in-line diode array UV detector was used to conduct high-performance liquid chromatography (HPLC) analyses. An Agilent Zorbax 300SB-C18 RP column, together with CH₃CN and water (both containing 0.1% trifluoroacetic acid (TFA)) as the eluent, was used for HPLC analysis.