A total of 5×105 MGC803 cells/well were seeded in 6-well plates and incubated overnight at 37°C. Following a 48 h reaction with 40 µM IBC, cells from the negative control and treatment groups were collected and centrifuged at 300 × g for 5 min. Cell smears were subsequently prepared and stained with Wright-Giemsa solution (Sigma-Aldrich) for 10 min, washed with running water and dried. Cell morphological changes were observed and images were captured using a CKX31 light microscope (Olympus Corp., Tokyo, Japan).
Ckx31 light microscope
Manufactured by Olympus
Sourced in Japan
The CKX31 is a compact and versatile light microscope designed for routine observation and analysis. It features a sturdy construction, high-quality optics, and a simple operation, making it suitable for a variety of laboratory applications. The CKX31 provides clear and detailed images, allowing users to observe samples with ease.
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Lab products found in correlation
3 protocols using ckx31 light microscope
1
Evaluating IBC-Induced Morphological Changes
2
Histological Analysis of Rat Liver Tissue
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Following sacrifice, the livers of the rats were excised, and the liver wet weights were measured. A section of hepatic tissue (~3×3×10 mm) was removed via a horizontal incision 1 cm away from the edge of the right lobe. The hepatic tissue samples were fixed in 10% formalin, dehydrated with a graded series of ethanol, ethanol uranyl acetate and acetone (all from Sinopharm Co., Ltd.), and stained with hematoxylin and eosin (H&E; n=3 rats/group). Observation and image capture were conducted using a CKX31 light microscope (Olympus Corporation, Tokyo, Japan).
3
Immunohistochemical Detection of HO-1
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Fresh frozen sections were thawed, dried, and then immersed in precooled acetone (−20°C) for 10 min. Endogenous peroxidase activity was blocked by 3% (v/v) H2O2, and the antigen was retrieved by microwave in 0.01 mol/L citrate buffer. Sections were then washed in PBS (0.1 mol/L). Goat polyclonal anti-HO-1 (Santa Cruz Biotechnology, CA, USA) and mouse anti-goat (Santa Cruz Biotechnology, CA, USA) occluding secondary antibodies were applied at 1 : 100 and incubated overnight at 4°C. Sections were washed four times in PBS for 20 min. All slides were analyzed blindly with respect to treatment condition, using an Olympus CKX31 light microscope (Olympus America Inc., Tokyo, Japan).
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